Effects of short-term exposure to volatile pesticide dichlorvos on the olfactory systems in Spodoptera litura: Calcium homeostasis, synaptic plasticity and apoptosis.

Volatile pesticides are a growing environmental and public health concern. However, little attention has been paid to its olfactory neurotoxic effect on pests and non-target organisms. Dichlorvos is a widely used organophosphorus fumigant that is ubiquitous in the environment. This study aims to explore the mode of action of the volatile dichlorvos-mediated olfactory impairment using a lepidopteran insect Spodoptera litura as a model. It was indicated that electroantennogram amplitudes of the male moths' response to sex pheromones and phenylacetaldehyde were reduced by approximately 20 % after 12-h fumigation exposure. RNA-Sequencing analysis revealed that down-regulation of trypsin and CLIC2 might be responsible for inhibition of odor recognition in the antenna, the peripheral olfactory tissue. In the head, 822 (84.05 %) of the 978 differentially expressed genes (DEGs) were up-regulated, of which seven DEGs encoding transcription factors may mainly modulate the stress-regulatory networks. Combining transcriptome with brain calcium imaging and Annexin V-mCherry staining experiments showed that volatile dichlorvos mainly disrupts Ca2+ homeostasis and synaptic plasticity, induces apoptosis in the central nervous system, and further leads to olfactory dysfunction. Overall, this study highlighted a comprehensive work model for dichlorvos-induced olfactory impairment in S. litura and may provide insights into toxic effects of airborne organophosphates on non-target organisms.

Olfactory dysfunction and potential mechanisms caused by volatile organophosphate dichlorvos in the silkworm as a model animal.

Volatile pesticides impair olfactory function in workers/farmers and insects, but data on molecular responses and mechanisms are poorly understood. This study aims to reveal the mechanisms of olfactory dysfunction in the silkworm after exposure to volatile dichlorvos. Our results demonstrated that acute exposure for 12 h significantly reduced electroantennogram responses, and over 62.50% of the treated male moths cannot locate the pheromone source. Transcriptional and proteomic responses of the antennae and heads were investigated. A total of 101 differentially expressed genes (DEGs) in the antennae, 138 DEGs in the heads, and 43 differentially expressed proteins (DEPs) in the heads including antennae were revealed. We discovered that upregulations of Arrestin1 and nitric oxide synthase1 (NOS1) may inhibit cyclic nucleotide-gated channels and hinder calcium influx in the antennae. In the central nervous systems (CNS), downregulations of tyrosine hydroxylase (TH) and tyrosine decarboxylase (TDC) may inhibit olfactory signal transduction by reducing the second messenger biosynthesis. Meanwhile, an abnormal increase of brain cell apoptosis was revealed by Annexin V-mCherry staining, often leading to persistent neurologic impairment. Taken together, this study highlighted olfactory dysfunction caused by dichlorvos, which may provide a novel perspective for understanding the toxicity mechanism of volatile pesticides in other organisms.

A Comparison of Co-expression Networks in Silk Gland Reveals the Causes of Silk Yield Increase During Silkworm Domestication.

Long-term domestication and selective breeding have increased the silk yield of the domestic silkworm (Bombyx mori) by several times the amount of the silk yield of its wild ancestor (Bombyx mandarina). However, little is known about the molecular mechanisms behind the increase in silk yield during domestication. Based on dynamic patterns of functional divergence in the silk gland between domestic and wild silkworms, we found that at early and intermediate stages of silk gland development, the up-regulated genes of the domestic silkworm were mainly involved in DNA integration, nucleic acid binding, and transporter activity, which are related to the division and growth of cells. This has led to the posterior silk gland (PSG) of the domestic silkworm having significantly more cells ("factories" of fibroin protein synthesis) than that of the wild silkworm. At the late stage of silk gland development, the up-regulated genes in the domestic silkworm was enriched in protein processing and ribosome pathways, suggesting protein synthesis efficiency is greatly improved during silkworm domestication. While there was an increase in fibroin protein synthesis, the production of sericin protein was simultaneously reduced in the silk gland of the domestic silkworm. This reflects that domestic and wild silkworms have been under different selection pressures. Importantly, we found that the network co-expressed with the silk-coding genes of the domestic silkworm was larger than that of the wild silkworm. Furthermore, many more genes co-expressed with silk-coding genes in the domestic silkworm were subjected to artificial selection than those in the wild silkworm. Our results revealed that the increase of silk yield during silkworm domestication is involved in improvement of a biological system which includes not only expansion of "factories" (cells of PSG) of protein synthesis, but also a high expression of silk-coding genes and silk production-related genes such as biological energy, transport, and ribosome pathway genes.