Loaches of Vanmanenia (Cypriniformes: Gastromyzontidae) from Yunnan, China with Description of a New Species.

After comparing specimens of Vanmanenia collected from the Lancang-jiang (the upper Mekong River), Yuan-jiang (the upper Red River), Lixian-jiang (first branch of the Red River), and Jinsha-jiang (the upper Yangtze River) drainages in Yunnan, China, we considered the specimens of Vanmanenia from Shunbi Township, Yangbi County, Yunnan (a branch of the Lancang-jiang drainage basin, the upper Mekong River) a new species, herein named V. microcephala sp. nov. Our investigation also suggested that V. striata should be restored as a valid species. On the basis of the systematic comparison of morphological characteristics of the genus Vanmanenia and based on the combination of the characteristics of the rostral fold, rostral barbels, the bars/marks on the flank, the rows of spots on the paired fins, and the markings on the caudal-fin base, the loaches of Vanmanenia should be divided into three groups: 1) the barred group, characterized by bars on the flank, 2), the cusped rostral fold group, characterized by the rostral fold lobes forming a cusp process, and 3) the barbeled rostral fold group, characterized by the rostral fold lobes specialized as secondary barbels. The barred group differs from congeners in Vanmanenia by the following combination of characters: three rostral lobes with a smooth and arcuate outer edge, a pattern of bars on the flank, and spotless paired fins. This group includes six species: V. crassicauda, V. microcephala sp. nov., V. serrilineata, V. striata, V. tetraloba, and Vanmanenia pseudostriata. The new species, V. microcephala, differs from the other species in the barred group by the following combination of characters: the lateral side of the body with 14-22 vermiculations with widths smaller than the diameter of the eye; the dorsal side of the head covered with a large black blotch; the gill opening smaller and its upper angle aligned with the lower edge of the eye; and the head smaller, head depth 45.2-47.1% head length. The characteristic evolutionary trends, origin, and taxonomic status of the barred group in the genus Vanmanenia are also discussed.

Loaches of Homatula (Teleostei: Nemacheilidae) from the upper Salween River in Yunnan, China with description of three new species.

Seven loaches of Homatula are distributed in a narrow geographical area between the upper Black River (or Song Da in Vietnam, that is a major tributary of the Red River, including the tributaries Lixian-jiang and Tengtiao-jiang in Yunnan, China) and the upper Salween River drainage (including tributaries of the Nu-jiang and Nanding-he, the latter is a major tributary of the Salween River in Yunnan, China). These seven species are distinguished from other Homatula by the combination of having the body densely-scaled, the lateral line complete, and a short adipose crest along the dorsal midline of the caudal peduncle that does not reach the posterior end of anal-fin base or does not extend beyond the middle of the anal-fin base. Based on a comparison with the described species of the densely-scaled group of Homatula, we can confirm that the specimens collected from the Nu-jiang drainage in western Yunnan are different from known species and are described herein as Homatula anteridorsalis sp. nov., Homatula cryptoclathrata sp. nov., and Homatula nigra sp. nov.

[Establishment of response system to emergency parasitic disease affairs in China].

China's prevention and control of parasitic diseases has made remarkable achievements. However, the prevalence and transmission of parasitic diseases is impacted by the complicated natural and social factors of environment, natural disasters, population movements, and so on. Therefore, there are still the risks of the outbreak of emergency parasitic diseases affairs, which may affect the control effectiveness of parasitic diseases and endanger the social stability seriously. In this article, we aim at the analysis of typical cases of emergency parasitic disease affairs and their impacts on public health security in China in recently years, and we also elaborate the disposal characteristics of emergency parasitic disease affairs, and propose the establishment of response system to emergency parasitic disease affairs in China, including the organizational structure and response flow path, and in addition, point out that, in the future, we should strengthen the system construction and measures of the response system to emergency parasitic disease affairs, so as to control the risk and harm of parasitic disease spread as much as possible and to realize the early intervention and proper disposal of emergency parasitic disease affairs.

Speckle-type POZ (pox virus and zinc finger protein) protein gene deletion in ovarian cancer: Fluorescence in situ hybridization analysis of a tissue microarray.

The aim of the present study was to investigate the status of speckle-type POZ (pox virus and zinc finger protein) protein (SPOP) gene located on chromosome 17q21 in ovarian cancer (OC). The present study evaluated a tissue microarray, which contained 90 samples of ovarian cancer and 10 samples of normal ovarian tissue, using fluorescence in situ hybridization (FISH). FISH is a method where a SPOP-specific DNA red fluorescence probe was used for the experimental group and a centromere-specific DNA green fluorescence probe for chromosome 17 was used for the control group. The present study demonstrated that a deletion of the SPOP gene was observed in 52.27% (46/88) of the ovarian cancer tissues, but was not identified in normal ovarian tissues. Simultaneously, monosomy 17 was frequently identified in the ovarian cancer tissues, but not in the normal ovarian tissues. Furthermore, the present data revealed that the ovarian cancer histological subtype and grade were significantly associated with a deletion of the SPOP gene, which was assessed by the appearance of monosomy 17 in the ovarian cancer samples; the deletion of the SPOP gene was observed in a large proportion of serous epithelial ovarian cancer (41/61; 67.21%), particularly in grade 3 (31/37; 83.78%). In conclusion, deletion of the SPOP gene on chromosome 17 in ovarian cancer samples, which results from monosomy 17, indicates that the SPOP gene may serve as a tumor suppressor gene in ovarian cancer.

Curcumin suppresses migration and invasion of human endometrial carcinoma cells.

Curcumin, a widely used Chinese herbal medicine, has historically been used in anti-cancer therapies. However, the anti-metastatic effect and molecular mechanism of curcumin in endometrial carcinoma (EC) are still poorly understood. The purpose of this study was to detect the anti-metastatic effects of curcumin and the associated mechanism(s) in EC. Based on assays carried out in EC cell lines, it was observed that curcumin inhibited EC cell migration and invasion in vitro. Furthermore, following treatment with curcumin for 24 h, there was a decrease in the expression levels of matrix metalloproteinase (MMP)-2 and -9 as well as proteinase activity in EC cells. Moreover, curcumin treatment significantly decreased the levels of the phosphorylated form of extracellular signal-regulated kinase (ERK) 1/2. MEK1 overexpression partially blocked the anti-metastatic effects of curcumin. Combined treatment with ERK inhibitor U0126 and curcumin resulted in a synergistic reduction in MMP-2/-9 expression; the invasive capabilities of HEC-1B cells were also inhibited. In conclusion, curcumin inhibits tumor cell migration and invasion by reducing the expression and activity of MMP-2/9 via the suppression of the ERK signaling pathway, suggesting that curcumin is a potential therapeutic agent for EC.

Effects and mechanisms of blocking the hedgehog signaling pathway in human gastric cancer cells.

Excessive activation of the hedgehog (Hh) signaling pathway is important in a variety of human cancer cell types, including gastric cancer. However, the underlying mechanisms of the Hh signaling pathway in inducing gastric tumorigenesis and its downstream target genes are largely unknown. In the present study, the inhibitory effect of cyclopamine on the Hh signaling pathway was investigated in the human gastric cancer AGS cell line. It was identified that cyclopamine treatment inhibited the proliferation, migration and invasion of the AGS cells in a dose- and time-dependent manner, and resulted in the downregulation of a number of key Hh signaling pathway-associated factors [glioma-associated oncogene homolog 1, C-X-C chemokine receptor type 4 and transforming growth factor (TGF)-ß1] at the RNA and protein levels. Furthermore, the secretion of TGF-ß1 was significantly reduced following the administration of cyclopamine to the AGS cells. The results of the present study provided insight into the mechanisms by which the Hh signaling pathway regulates gastric cancer formation and identified the Hh signaling pathway as a potential novel therapeutic target in human gastric cancer.