Research on the site selection of emergency medical facilities from the perspective of country parks.

Post-epidemic era, human society entered the stage of epidemic prevention and control normalized, emergency medical facilities are an important means of epidemic prevention and control, attention-needed can provide construction sites for emergency medical facilities. Disaster prevention and green space can provide construction sites for emergency medical facilities. In comparison, it was found that the site selection conditions of country parks and emergency medical facilities were more compatible. Based on the requirement of the latter's location, using the Delphi method and analytic hierarchy process, through to the country park type, effective safety area, space fragmentation, distance away from the water, in the wind, away from the city center distance, hydrogeology, eight factors such as traffic is quantitative, construct the evaluation system of emergency medical facility location. Taking Nanjing as an example, the Nanjing city region within the scope of the 43 country parks comprehensive comparison. Results show that green hill forest park, the highest scores in addition to the traffic time factor, the remaining seven factor score the highest, the most balanced distribution, security, scalability, rehabilitation, convenience, pollution prevention, and evacuation. It can be preferred as the site selection and construction target of emergency medical facilities. Besides, the Youzishan Forest Park and Dongkeng Country Park comprehensive scores and each factor score is higher, can be used as emergency preplan alternative goals. The score results indicate that the evaluation method for severity exhibits higher levels of differentiation, significant validity, and highly consistent assignment of impact factor weights. In view of the different regional land, impact factor weights assignment can be adjusted adjust measures to local conditions, to effectively make use of the existing conditions, avoid adverse factors.

A novel method to fabricate thermoresponsive microstructures with improved cell attachment/detachment properties.

A novel, simple, and rapid method to fabricate thermoresponsive micropatterned substrate for cell adhesion, growth, and thermally induced detachment was developed. Thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAAm), was grafted onto the surface of polystyrene (PS) film with microstructure by plasma-induced graft polymerization technique. The thermoresponsive micropatterned films were characterized by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, hydrogen nuclear magnetic resonance ((1) H NMR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). These results indicated that the grafting ratio of PNIPAAm increased with increasing roughness of PS film. However, the microstructures on the substrate were not affected by grafted PNIPAAm. The optimal grafting conditions, such as plasma treatment time, monomer concentration, graft polymerization time, and graft medium were investigated. The thermoresponsive micropatterned films were investigated with the fibroblast cell (L929) adhesion, proliferation, and thermally induced detachment assay. The microstructure on the thermoresponsive micropatterned substrate facilitated cell adhesion above the lower critical solution temperature (LCST) of PNIPAAm and cell detachment below the LCST. Moreover, it can be used to regulate cell organization and tissue growth.