A novel laser-EMAT ultrasonic longitudinal wave resonance method for wall thickness measurement at high temperatures.

In this paper we propose a novel ultrasonic longitudinal wave resonance method for measuring the thickness of metal walls using a laser-electromagnetic acoustic transducer (Laser-EMAT). The method is based on the surface constraint mechanism (SCM) of the material and is expected to be capable of accurately detecting local thinning of metal walls in a non-contact manner and at high temperatures. Based on finite element analysis of laser-EMAT ultrasonic resonance measurement of aluminum alloy thickness, we investigated the effects of such key factors as SCM, irradiation parameters of laser source, and the size of EMAT receiving coil on the accuracy of thickness measurement (resonance frequency position) and on the amplitude of the resonance wave. Both numerical simulations and experiments are conducted to demonstrate that the measurement accuracy of the proposed method is not affected by SCM, irradiation laser source parameters, and EMAT receiving coil size, and that accurate detection of stepped aluminum plates with thickness thinning from 3.0 mm to 0.5 mm is achieved. Furthermore, we were able to perform rapid detection of aluminum thin plate thickness at 500 °C temperature with an EMAT lift-off of 5.0 mm and achieved a relative experimental error as small as 3.40 %. The results obtained in this study showed that the proposed method performed well in non-contact measurement of metal thinning in harsh environment of high temperature.

Ultrasonic backscattering model for Rayleigh waves in polycrystals with Born and independent scattering approximations.

This paper presents theoretical and numerical models for the backscattering of 2D Rayleigh waves in single-phase, untextured polycrystalline materials with statistically equiaxed grains. The theoretical model, based on our prior inclusion-induced Rayleigh wave scattering model and the independent scattering approximation, considers single scattering of Rayleigh-to-Rayleigh (R-R) waves. The numerical finite element model is established to accurately simulate the scattering problem and evaluate the theoretical model. Good quantitative agreement is observed between the theoretical model and the finite element results, especially for weakly scattering materials. The agreement decreases with the increase of the anisotropy index, owing to the reduced applicability of the Born approximation. However, the agreement remains generally good when weak multiple scattering is involved. In addition, the R-R backscattering behaviour of 2D Rayleigh waves is similar to the longitudinal-to-longitudinal and transverse-to-transverse backscattering of bulk waves, with the former exhibiting stronger scattering. These findings establish a foundation for using Rayleigh waves in the quantitative characterisation of polycrystalline materials.

Therapeutic effect of PD-1 monoclonal antibody combined with cisplatin or gemcitabine chemotherapy in a mouse xenograft model with KRAS mutant non-small cell lung cancer A549 cells / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：探讨程序性死亡受体-1（PD-1）单抗联合顺铂或吉西他滨在KRAS基因突变非小细胞肺癌（NSCLC）A549细胞移植瘤小鼠模型治疗中的作用。方法：构建免疫系统-肿瘤双人源化A549细胞小鼠移植瘤模型，将60只小鼠按随机数字表法分成6组（10只/组），分别为对照组（200 μL/kg PBS）、PD-1单抗组（20 mg/kg PD-1单抗）、顺铂组（3 mg/kg顺铂）、PD-1单抗+顺铂组（20 mg/kg PD-1单抗+3 mg/kg顺铂）、吉西他滨组（30 mg/kg吉西他滨）和PD-1单抗+吉西他滨组（20 mg/kg PD-1单抗+30 mg/kg吉西他滨）。TUNEL和DAPI双染色法检测移植瘤组织中细胞凋亡水平，测量移植瘤体积和质量并计算肿瘤生长抑制率，免疫组化法检测移植瘤微血管密度（MVD）。结果：成功构建免疫系统-肿瘤双人源化NSCLC A549细胞小鼠移植瘤模型，PD-1单抗+顺铂组移植瘤的细胞凋亡率、肿瘤生长抑制率均最高，移植瘤体积、质量和MVD均最小，与其他5组小鼠比较差异均有统计学意义（均P<0.05）。结论：顺铂与PD-1单抗具有协同活性，而吉西他滨拮抗PD-1单抗的治疗作用。提示PD-1单抗联合顺铂对KRAS突变NSCLC A549细胞移植瘤小鼠的疗效更好。

Interaction of elastic waves in solids with quadratic and cubic nonlinearity.

This article investigates the interactions of two-plane waves in weakly nonlinear elastic solids containing quadratic and cubic nonlinearity. The analytical solutions for generated combined harmonic waves are derived using the Green's function approach applied to a generated system of quasi-linear equations of motion. Wave mixing solutions are obtained and include shape functions that permit closed-form solutions for a variety of interaction geometries. An explicit example is highlighted for a spherical interaction volume assuming isotropic elastic constants. Several parameters of the generated field after mixing are analyzed including resonant and nonresonant mixing, the role of interaction angle, and the frequencies of the two incident waves. Wave mixing offers the potential for sensing localized elastic nonlinearity and the present model can be used to help design experimental configurations.

Highly Sensitive Detection of Microstructure Variation Using a Thickness Resonant Transducer and Pulse-Echo Third Harmonic Generation.

In nonlinear ultrasound testing, the relative nonlinear parameter is conveniently measured as a sensitive means of detecting and imaging overall variation of microstructures and damages. Compared to the quadratic nonlinear parameter (ß'), the cubic nonlinear parameter (γ'), calculated as the third harmonic amplitude divided by the cube of the fundamental amplitude, has generally a higher value, providing better sensitivity in nonlinear parameter mapping. Since the third harmonic amplitude is about two orders of magnitude lower than the fundamental amplitude, efficient excitation and highly sensitive reception of third harmonic is very important. In this paper, we explore an odd harmonic thickness resonant transducer that meets the requirements for pulse-echo third harmonic generation (THG) measurements. We also address the problem of source nonlinearity that may be present in the measured amplitude of the third harmonic and propose a method to properly correct it. First, we measure γ' for a series of aluminum specimens using the through-transmission method to observe the behavior of γ' as a function of specimen thickness and input voltage, and examine the effects of various corrections such as attenuation, diffraction and source nonlinearity. Next, we apply the odd harmonic resonant transducer to pulse-echo THG measurements of precipitation heat-treated specimens. It is shown that such transducer is very effective in generation and detection of fundamental and third harmonics under finite amplitude toneburst excitation. The highly sensitive detectability of γ' are presented as a function of aging time, and the sensitivity of γ' is compared with that of ß' and ß'2.

Measurement and In-Depth Analysis of Higher Harmonic Generation in Aluminum Alloys with Consideration of Source Nonlinearity.

Harmonic generation measurement is recognized as a promising tool for inspecting material state or micro-damage and is an ongoing research topic. Second harmonic generation is most frequently employed and provides the quadratic nonlinearity parameter (ß) that is calculated by the measurement of fundamental and second harmonic amplitudes. The cubic nonlinearity parameter (ß2), which dominates the third harmonic amplitude and is obtained by third harmonic generation, is often used as a more sensitive parameter in many applications. This paper presents a detailed procedure for determining the correct ß2 of ductile polycrystalline metal samples such as aluminum alloys when there exists source nonlinearity. The procedure includes receiver calibration, diffraction, and attenuation correction and, more importantly, source nonlinearity correction for third harmonic amplitudes. The effect of these corrections on the measurement of ß2 is presented for aluminum specimens of various thicknesses at various input power levels. By correcting the source nonlinearity of the third harmonic and further verifying the approximate relationship between the cubic nonlinearity parameter and the square of the quadratic nonlinearity parameter (ß∗ß), ß2≈ß∗ß, the cubic nonlinearity parameters could be accurately determined even with thinner samples and lower input voltages.

Ordinary state-based peri-ultrasound modeling to study the effects of multiple cracks on the nonlinear response of plate structures.

Since it is almost impossible to carry out a comprehensive parametric investigation experimentally for internal cracks with different geometry and orientation, a good numerical modeling and simulation technique is necessary to have a clear understanding of the physics of wave propagation and its interaction with cracks. Such investigation is helpful for structural health monitoring (SHM) with ultrasonic techniques. This work presents a nonlocal peri-ultrasound theory based on ordinary state-based (OSB) peridynamics for modeling elastic wave propagation in three-dimensional (3-D) plate structures containing multiple cracks. A relatively new and promising nonlinear ultrasonic technique called Sideband Peak Count - Index (or SPC-I) is adopted to extract the nonlinearity generated from the interactions between elastic waves and multiple cracks. Effects of three main parameters - the distance between the acoustic source and the crack, the crack spacing and the number of cracks are investigated using the proposed OSB peri-ultrasound theory together with the SPC-I technique. For each of these three parameters investigation, different crack thicknesses were considered - 0 mm (crack-free), 1 mm (thin crack), 2 mm (intermediate thickness) and 4 mm (thick crack); thin and thick cracks are defined after comparing the crack thickness value with the horizon size mentioned in the peri-ultrasound theory. It is found that for obtaining consistent results the acoustic source should be placed at least one wavelength away from the crack and crack spacings also play an important role on the nonlinear response. It is concluded that the nonlinear response diminishes when the cracks become too thick, and thin cracks show higher nonlinearity than that of thick cracks and no cracks. Finally, the proposed method which is combining the peri-ultrasound theory and SPC-I technique is used for monitoring cracks' evolution process. The numerical modeling results are compared with the experimental findings reported in the literature. Consistent qualitative trends in SPC-I variations predicted numerically and obtained experimentally are observed, thus it gives confidence in the proposed method.

Theoretical and numerical modeling of Rayleigh wave scattering by an elastic inclusion.

This work presents theoretical and numerical models for the backscattering of two-dimensional Rayleigh waves by an elastic inclusion, with the host material being isotropic and the inclusion having an arbitrary shape and crystallographic symmetry. The theoretical model is developed based on the reciprocity theorem using the far-field Green's function and the Born approximation, assuming a small acoustic impedance difference between the host and inclusion materials. The numerical finite element (FE) model is established to deliver a relatively accurate simulation of the scattering problem and to evaluate the approximations of the theoretical model. Quantitative agreement is observed between the theoretical model and the FE results for arbitrarily shaped surface/subsurface inclusions with isotropic/anisotropic properties. The agreement is excellent when the wavelength of the Rayleigh wave is larger than, or comparable to, the size of the inclusion, but it deteriorates as the wavelength gets smaller. Also, the agreement decreases with the anisotropy index for inclusions of anisotropic symmetry. The results lay the foundation for using Rayleigh waves for quantitative characterization of surface/subsurface inclusions, while also demonstrating its limitations.

Modeling ultrasonic wave fields scattered by flaws using a quasi-Monte Carlo method: Theoretical method and experimental verification.

The modeling and visualization of wave fields scattered by flaws can be helpful in terms of guiding the testing and evaluation of flaws using an ultrasonic nondestructive method. In this work, the ultrasonic scattering of wave fields from flaws with different shapes is modeled using a quasi-Monte Carlo (QMC) method and measured through experiments for verification. The incident wave fields generated by a transducer can be modeled using the Rayleigh integral expression and calculated using the QMC method. When the size of the flaw is much larger than the wavelength, the incident wave over the lit portion of flaw can be treated as the source for the scattering of wave fields, and these wave fields can also be modeled using the proposed QMC method. In this paper, water is treated as the material and an embedded solid component is considered as the flaw. Numerical examples and results are presented for flaws with different shapes and sizes, and the properties of these scattering wave fields are analyzed and discussed. Experiments are performed to measure the scattering wave fields using a needle transducer, and it is shown that the results agree with the simulations, thus verifying the proposed modeling method. The work presented here can assist in understanding the wave-flaw interaction and can help in optimizing ultrasonic nondestructive testing.

Thermo-acoustoelastic effect of Rayleigh wave: Theory and experimental verification.

Previous studies showed that the thermally-induced ultrasonic bulk wave velocity change could be used to measure acoustoelastic coefficients and third-order elastic constants of elastic materials. This method is naturally immune from the ambient temperature effect and has improved sensitivity and a simpler test setup than the conventional acoustoelastic test. However, Rayleigh wave is preferred for thick components or structures with only one accessible surface. In this work, the thermo-hyperelastic constitutive equation, along with acoustoelastic theory, is used to derive the expression of the thermo-acoustoelastic coefficient (TAEC) of Rayleigh wave. The numerical relationship between the TAEC of Rayleigh wave and Murnaghan constants (l, m and n) are given for common metals. The TAEC expressions for Rayleigh wave and shear wave are similar, and both are dominated by the constant m. The TAEC of Rayleigh wave was measured on an aluminum 6061 specimen using the thermal modulation experiment in a temperature range of 22 ∼35 °C. The measured TAEC shows good agreement with the theoretical calculation. Then the third-order elastic constants were calculated based on TAECs of bulk waves and Rayleigh wave.

Finite Element Simulation of Ultrasonic Scattering by Rough Flaws with Multi-Scale Distortions.

The roughness of a flaw's surface significantly affects the scattering behavior of ultrasonic waves. It is vital to understand the impact of roughness on flaw echoes, especially when performing ultrasonic nondestructive inspection on safety-critical components. However, the current approach for creating rough flaw models fails to reconstruct complicated cracks with secondary cracks. Here, a multi-scale distortion method is developed to generate a rough flaw by using an optical microscope image of a real flaw. The finite element (FE) is then implemented to simulate the near-surface rough flaws in nickel-based bars, which are detected by an offsetting immersion transducer with mode-converted transverse waves. Numerical results show that the randomness and complexity of flaw echoes from rough flaws are exceptionally high. The gap between the maximum and minimum normalized amplitude values of flaw echoes from a rough crack with secondary cracks can reach 7.125 dB. Meanwhile, the maximum time of flight (TOF) is almost twice as large as the minimum TOF. Therefore, the present method can generate effective rough flaw models in terms of macroscopic rough geometry and microscopic rough surface. Moreover, the impact of the rough flaw surface on the flaw echoes goes beyond amplitude changes and may make flaw location challenging.

Attenuation and dispersion of leaky Rayleigh wave in polycrystals.

In this work, we use the characteristic equation of leaky Rayleigh waves (LRWs) and a unified approach of bulk waves proposed by Stanke and Kino [J. Acoust. Soc. Am. 75, 665-681 (1984)] to calculate the attenuation and velocity dispersion of LRWs in polycrystals. Numerical results demonstrate that the total attenuation including the leakage attenuation and scattering attenuation is proportional to frequency and independent of grain size in the Rayleigh scattering regime. Meanwhile, the variation of phase velocity in all scattering regimes remains at ∼0.7% according to the theoretical expectation; this means that the velocity dispersion of the LRWs can be ignored, consistent with the conventional viewpoint. Measurements are conducted on stainless steel at different ultrasonic frequencies (all in the Rayleigh scattering regime). The non-paraxial sound field model is used here to eliminate the diffraction loss and to obtain the total attenuation. Experimental results verify that LRWs have very little velocity dispersion. Meanwhile, experimental fitting data reveal that the modified theoretical model can be used to evaluate the total attenuation (only ∼2% discrepancies) of LRWs under the consideration of the diffraction effect. The relative errors between experimental scattering attenuation and theoretical value ranged from 11% to 18%, mainly owing to the effect of surface roughness and measurement inaccuracy.

Sideband peak count-index technique for monitoring multiple cracks in plate structures using ordinary state-based peri-ultrasound theory.

This work presents a peri-ultrasound theory based on ordinary state-based peridynamics for modeling elastic waves propagating in three-dimensional (3-D) plate structures and interacting with multiple cracks. A recently developed nonlinear ultrasonic technique called sideband peak count-index (or SPC-I) is adopted for monitoring one or more cracks with thickness values equal to 0 mm (crack-free), 1, 2, and 4 mm. Three separate scenarios-one crack, two cracks, and four cracks in 3-D plate structures-are investigated. These cracks can be classified as thin and thick cracks depending on the horizon size, which is mentioned in peri-ultrasound theory. Computed results for all three cases show larger SPC-I values for thin cracks than for thick cracks and the case of no cracks. This observation is in line with the previously reported results in the literature and proves that the state-based peri-ultrasound theory can capture the expected nonlinear response of elastic waves interacting with multiple cracks without changing the cracks' surface locations artificially, and this is always needed in most of the other numerical methods. The proposed state-based peri-ultrasound theory is more flexible and reliable for solving 3-D problems, and the out-of-plane wave field can be obtained for engineering analysis.

Modeling ultrasonic wave fields using a Quasi-Monte Carlo method: Wave transmission through complicated interfaces.

The sound fields generated by ultrasonic transducers can be modeled using the Quasi-Monte Carlo (QMC) method with a high level of accuracy and efficiency from Zhang [J. Acoust. Soc. Am. 149(1), 7-15 (2021)]. In this work, this method is extended to simulate transmitted wave fields through complicated interfaces. When a wave propagates in two-layer media, the vibrating waves over the interface radiated by the transducer can be treated as the source for generating waves in the second medium, thus, a nested-form Rayleigh integral expression can be used as a model equation for the transmitted wave calculation. When the QMC method is used to solve the nested integral, pseudo-random samples for constructing the transducer and the interface are sampled separately and the transmitted wave fields are obtained using the final sample mean. Numerical examples and results are presented when the wave transmits normally or obliquely through planar or curved interfaces. The results indicate that the high level of accuracy and efficiency remains when the QMC method is used to model the transmitted wave fields. One important advantage is that wave fields can be well simulated using the QMC method when the wave transmits through a complicated interface as long as the interface can be constructed using pseudo-random samples.

Fast Fourier transform method for determining velocities of ultrasonic Rayleigh waves using a comb transducer.

A convenient, accurate and precise method is proposed to determine velocities of ultrasonic Rayleigh waves in different materials by extracting central frequencies of signals, which are measured by a comb transducer and converted to the frequency domain using the fast Fourier transformation (FFT). The velocities can be calculated as cr = fl, where f is the central frequency of the wave signal and l is the teeth spacing or period of the comb transducer. The experimental measurements are easy to do, as long as the Rayleigh wave reflected from the standard reflectors are measured using one comb transducer, without knowing the wave propagation distances and times. Results show that the proposed technique has a high level of precision, as the central frequencies are very stable. The same comb transducer is used to measure the Rayleigh wave velocities in different materials where the velocities vary from 2100 m/s to 3400 m/s. Comparison of the experimental results with those measured using the time-of-flight method showed a high level of accuracy - all relative errors were found to be less than 1%.

Evaluating elongated grains with diffuse ultrasonic double scattering and rectangular transducers.

Diffuse scattering of ultrasound by the microstructure of polycrystal specimens can be used to evaluate grain size and grain elongation. The existing diffuse scattering models mostly dealt with circular transducers whose symmetrical sound field is insensitive to the asymmetric elongated grain. The sound field of a rectangular transducer provides a new perspective for acquiring additional information. First, the existing single scattering response (SSR) and double scattering response (DSR) models are modified for a rectangular transducer, where the sound field of a rectangular transducer is equivalent to that of an elliptical transducer in the far-field. Therefore, an equivalent single Gaussian beam model is derived using amplitude-equivalent and beamwidth-equivalent coefficients. Then, the spatial correlation function of elongated grains is transformed into the wavenumber domain, giving rise to the SSR and DSR of a rectangular transducer that reveals the interaction effect of an asymmetric sound field and elongated grains on ultrasonic backscattering. The experimental results show that the sizes of elongated grains in a cold-rolled aluminum are evaluated as 1086 ± 8, 90 ± 4, and 10 ± 1 µm in the x, y, and z directions, where the exact values are 1184.2 ± 11.9, 80.7 ± 5.2, and 8.3 ± 0.5 µm according to metallographic measurements.

Subwavelength ultrasonic imaging using a deep convolutional neural network trained on structural noise.

Subwavelength ultrasonic imaging (SUI) can detect subwavelength flaws beyond the diffraction limit, however, SUI sometimes fails to clearly reveal flaws in C-scans when the signal-to-noise ratio (SNR) is low. In this work, a convolutional neural network (CNN) that takes structural noise into account is developed for SUI to distinguish flaw echoes from structural noise. The network contains a regression CNN for learning features from the structural noise and a learnable soft thresholding layer for classification. Experiments show that the proposed method performs well for imaging subwavelength flaws at different depths and of different sizes. It achieved an F1 score of 97.69 ± 1.56% in detecting flaws as compared to the enhanced ultrasonic flaw detection method with time-dependent threshold. As an example of general application of the method, we also performed SUI on natural flaws in a spheroidal graphite cast iron specimen. The results show that the method can achieve SUI without a theoretical backscattering model and is not limited by noise distribution, multiple scattering, or complex microstructures. Furthermore, the network does not need to prepare flaw echoes for training.

Determining the Responsivity of Air-Coupled Piezoelectric Transducers Using a Comparative Method: Theory and Experiments.

The responsivity of an ultrasonic transducer is an important parameter in evaluating its effective frequency band, the electroacoustic conversion efficiency, and the measurement capability of the system. The determination of the responsivity of a traditional immersion or contact piezoelectric transducer has been well investigated. However, due to the high attenuation of waves propagating in air and the large acoustic impedance mismatch between the active piezoceramic material and the load medium, there are few reports of the calibration of an air-coupled piezoelectric transducer. In this work, we present a comparative method of measuring the responsivity of an air-coupled transducer: the air-coupled transducer is used to receive a broadband pulse signal to evaluate its frequency spectrum, and a toneburst signal with known vibration displacement is measured by the air-coupled transducer in order to calibrate the amplitude of the responsivity. The effects of transmitter responsivity, input pulse characteristics, attenuation, and diffraction are taken into account to improve the accuracy of the responsivity determination. In addition, the measurement of the amplitude of the responsivity by comparing the measured displacements avoids the complicated task of characterizing the effects of electrical equipment. The determined responsivity is checked by comparing the measured displacements using different methods at different frequencies in order to evaluate its frequency spectrum and by measuring the nonlinearity parameters of the material to evaluate its amplitude. The agreement between results obtained using different methods demonstrates that the calibrated responsivity of the air-coupled transducer is valid, and the proposed method is effective.

Absolute Measurement of Material Nonlinear Parameters Using Noncontact Air-Coupled Reception.

Nonlinear ultrasound is often employed to assess microdamage or nonlinear elastic properties of a material, and the nonlinear parameter is commonly used to quantify damage sate and material properties. Among the various factors that influence the measurement of nonlinear parameters, maintaining a constant contact pressure between the receiver and specimen is important for repeatability of the measurement. The use of an air-coupled transducer may be considered to replace the contact receiver. In this paper, a method of measuring the relative and absolute nonlinear parameters of materials is described using an air-coupled transducer as a receiver. The diffraction and attenuation corrections are newly derived from an acoustic model for a two-layer medium and the nonlinear parameter formula with all corrections is defined. Then, we show that the ratio of the relative nonlinear parameter of the target sample to the reference sample is equal to that of the absolute nonlinear parameter, and this equivalence is confirmed by measurements on three systems of aluminum samples. The proposed method allows the absolute measurement of the nonlinear parameter ratio or the nonlinear parameter without calibration of the air-coupled receiver and removes restrictions on the selection of reference samples.

Modeling of wave fields generated by ultrasonic transducers using a quasi-Monte Carlo method.

The sound fields generated by ultrasonic transducers are modeled using the quasi-Monte Carlo (QMC) method, which is found to overcome the conflict between accuracy and efficiency that occurs in existing wave field calculation methods. The RI equation, which is frequently used as a model equation in ultrasonic field calculation, is used here as an exact method and for comparison purposes. In the QMC method, the judgment sampling method and Halton sequence are used for pseudo-random sampling from the sound source, and then the sound field distributions are found by solving the integral solution using the sample mean. Numerical examples and results are presented when modeling unfocused, focused, and steered and focused beam fields. The accuracy and efficiency of the QMC method are discussed by comparing the results obtained using different modeling methods. The results show that the proposed method has a high level of efficiency due to the nature of the QMC algorithm and a high level of accuracy because no approximation is required. In addition, wave fields can be modeled with the QMC method as long as sound sources can be effectively pseudo-randomly sampled, allowing the proposed method to be applied to various types of transducers.