ABSTRACT
OBJECTIVE: This study is aimed at studying the effect of quercetin on the Alzheimer disease cell model induced by Aß 25-35 in PC12 cells and its mechanism of action. METHODS: The AD cell model was established by Aß 25-35. Quercetin was used at different concentrations (0, 10, 20, 40, and 80 µmol/L). The morphology of cells was observed, and the effect on cell survival rate was detected by the MTT method. Cell proliferation was detected by the SRB method. The contents of LDH, SOD, MDA, GSH-Px, AChE, CAT, and T-AOC were detected by kits. The expression of sirtuin1/Nrf2/HO-1 was detected by RT-qPCR and Western blot. RESULTS: PC12 cells in the control group grew quickly and adhered well to the wall, most of which had extended long axons and easily grew into clusters. In the model group, cells were significantly damaged and the number of cells was significantly reduced. It was found that PC12 cells were swollen, rounded, protruding, and retracting, with reduced adherent function and floating phenomenon. Quercetin could increase the survival rate and proliferation rate of PC12 cells; reduce the levels of LDH, AChE, MDA, and HO-1 protein; and increase the levels of SOD, GSH-Px, CAT, T-AOC, sirtuin1, and Nrf2 protein. CONCLUSION: Quercetin can increase the survival rate of PC12 injured by Aß 25-35, promote cell proliferation, and antagonize the toxicity of Aß; it also has certain neuroprotective effects. Therefore, quercetin is expected to become a drug for the treatment of AD.
Subject(s)
Alzheimer Disease/metabolism , Amyloid beta-Peptides/metabolism , Heme Oxygenase (Decyclizing)/metabolism , Models, Neurological , NF-E2-Related Factor 2/metabolism , Peptide Fragments/metabolism , Quercetin/pharmacology , Signal Transduction/drug effects , Sirtuin 1/metabolism , Alzheimer Disease/genetics , Alzheimer Disease/pathology , Amyloid beta-Peptides/genetics , Animals , Cell Survival/drug effects , Cell Survival/genetics , Heme Oxygenase (Decyclizing)/genetics , NF-E2-Related Factor 2/genetics , PC12 Cells , Peptide Fragments/genetics , Rats , Signal Transduction/geneticsABSTRACT
The selection of scaffold materials and the optimization of scaffold morphological and mechanical properties are critical for successful bone tissue engineering. We fabricated porous scaffolds of nano-sized zirconia using a replication technique. The study aimed to explore the relationship between porosity, pore size, mechanical strength, cell adhesion, and cell proliferation in the zirconia scaffolds. Macro- and micro-structures and compressive strength were comparatively tested. Beagle bone marrow stromal cells were seeded onto the scaffolds to evaluate cell seeding efficiency and cell proliferation profile over 14 d of incubation. The zirconia scaffolds presented a complex porous structure with good interconnectivity of pores. By increasing the sinter cycles, the porosity and pore size of the scaffolds decreased, with mean values ranging from 92.7-68.0% and 830-577 µm, respectively, accompanied by increased compressive strengths of 0.6-4.4 MPa. Cell seeding efficiency and cell proliferation over the first 7 d of incubation increased when the porosity decreased, with cell viability highest in the scaffold with a porosity of 75.2%. After 7 d of incubation, the cell proliferation increased when the porosity increased, highest in the scaffolds with a porosity of 92.7%. These results showed that the zirconia scaffold with a porosity of 75.2% possesses favorable mechanical and biological properties for future applications in bone tissue engineering.
