ABSTRACT
A new method for the determination of arecoline in Semen Arecae decoction pieces by microchip capillary electrophoresis with contactless conductivity detection (MCE-CCD) was proposed. The effects of various electrophoretic operating parameters on the analysis of arecoline were studied. Under the optimal conditions, arecoline was rapidly separated and detected in 1 min with good linearity over the concentration range of 20-1500 µM (r2=0.9991) and the detection limit of 5 µM (S/N=3). The method was used for the analysis of arecoline satisfactorily with a recovery of 96.8-104%.
ABSTRACT
OBJECTIVE: To establish a new method for the determination of matrine in matrine injection by microfluidic chip with contactless conductivity detection. METHODS: The electrophoretic parameters, such as the variety and concentration of buffer solution and additive, separation voltage and injection time, etc. were researched. RESULTS: In the optimal conditions, using 2 mmol/L HAc + 2 mmol/L NaAc (pH 4. 5) as buffer solution, with 0.20 mmol/L SDS as addition at the separation voltage of 2.00 kV and 10s injection, matrine was separated and detected within 2 min. The linear dependence of the concentration of matrine ranged from 10 to 300 microg/mL (r2 = 0.9992), with the detection limit of 1.0 microg/mL (S/N = 3), the RSD of 1.9% and the recoveries from 98.7% to 101%. CONCLUSION: The method is simple, rapid and well reproducible, and it can be used in the quality control of the product.
