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1.
Int Immunopharmacol ; 136: 112338, 2024 Jul 30.
Article in English | MEDLINE | ID: mdl-38850787

ABSTRACT

Cardiac fibrosis is a typical feature of cardiac pathological remodeling, which is associated with adverse clinical outcomes and has no effective therapy. Nicotine is an important risk factor for cardiac fibrosis, yet its underlying molecular mechanism remains poorly understood. This study aimed to identify its potential molecular mechanism in nicotine-induced cardiac fibrosis. Our results showed nicotine exposure led to the proliferation and transformation of cardiac fibroblasts (CFs) into myofibroblasts (MFs) by impairing autophagy flux. Through the use of drug affinity responsive target stability (DARTS) assay, cellular thermal shift assay (CETSA), and surface plasmon resonance (SPR) technology, it was discovered that nicotine directly increased the stability and protein levels of lactate dehydrogenase A (LDHA) by binding to it. Nicotine treatment impaired autophagy flux by regulating the AMPK/mTOR signaling pathway, impeding the nuclear translocation of transcription factor EB (TFEB), and reducing the activity of cathepsin B (CTSB). In vivo, nicotine treatment exacerbated cardiac fibrosis induced in spontaneously hypertensive rats (SHR) and worsened cardiac function. Interestingly, the absence of LDHA reversed these effects both in vitro and in vivo. Our study identified LDHA as a novel nicotine-binding protein that plays a crucial role in mediating cardiac fibrosis by blocking autophagy flux. The findings suggest that LDHA could potentially serve as a promising target for the treatment of cardiac fibrosis.


Subject(s)
Autophagy , Fibrosis , Nicotine , Animals , Autophagy/drug effects , Rats , Male , Rats, Inbred SHR , Signal Transduction/drug effects , Myocardium/pathology , Myocardium/metabolism , Lactate Dehydrogenase 5/metabolism , Cells, Cultured , Humans , Fibroblasts/drug effects , Fibroblasts/metabolism , TOR Serine-Threonine Kinases/metabolism , Myofibroblasts/drug effects , Myofibroblasts/metabolism , Rats, Sprague-Dawley
2.
Shanghai Kou Qiang Yi Xue ; 32(2): 220-224, 2023 Apr.
Article in Chinese | MEDLINE | ID: mdl-37154008

ABSTRACT

PURPOSE: To integrate digital dynamic smile aesthetic simulation (DSAS) cognitive education in orthodontic practicum and evaluate the teaching effects. METHODS: A total of 32 dental students during orthodontic practicum were randomly divided into two groups. One group received traditional teaching method to draft treatment plan, and another group was implemented with DSAS teaching method. Then two groups exchanged. Students were asked to grade both teaching methods and statistical analysis was performed on the scoring results with SPSS 24.0 software package. RESULTS: The scores of the DSAS teaching method was much higher than traditional method, and the difference was statistically significant(P=0.012). Students considered that DSAS teaching method was more "novel and fascinating", and also "convenient for comprehending of orthodontic treatment". Students hoped to popularize the DSAS teaching method in future orthodontic practicum. CONCLUSIONS: As a novel teaching method, DSAS is more intuitive and vivid to stimulate students' interest in learning, and it is helpful to improve the effect of orthodontic practical teaching.


Subject(s)
Esthetics, Dental , Learning , Humans , Clinical Competence , Dental Care , Students , Teaching
3.
Biochem Biophys Res Commun ; 524(1): 77-82, 2020 03 26.
Article in English | MEDLINE | ID: mdl-31980179

ABSTRACT

OBJECTIVES: Protein arginine methyltransferase 2 (PRMT2) is closely related to the occurrence and development of atherosclerosis. However, its underlying mechanisms remain to be elucidated. The purpose of this study is to observe the effect of overexpression of PRMT2 on the formation of foam cells and to explore its possible mechanism in RAW 264.7 macrophage. METHODS: Lentivirus vector of overexpression PRMT2 (LV-PRMT2) was constructed. LV-PRMT2 and lentivirus vector GV492 were transfected into RAW 264.7 macrophages, positive clone cells were screened by treatment with 4.0 µg/mL puromycin for 4 weeks. The macrophages were treated with ox-LDL (50 µg/mL) for 48 h to induce foaming. The lipid accumulation of macrophages was observed by oil red O staining. The levels of cellular total cholesterol (TC), free cholesterol (FC) and cholesteryl ester (CE) were measured by high performance liquid chromatography (HPLC) assays. The cholesterol efflux of macrophages was tested by the [3H] labeled cholesterol. The expressions of ATP binding cassette transporter A1 (ABCA1), ATP binding cassette transporter G1 (ABCG1), CD36 and scavenger receptor A1 (SR-A1) in macrophages were measured by Western Blot. RESULTS: The results showed that LV-PRMT2 and lentivirus vector has been successfully transfected into RAW 264.7 macrophage. Compared with the Vector group, the mRNA and protein expressions of PRMT2 were significantly up-regulated (P < 0.05). Compared with Control group, the expression of PRMT2 was significantly down-regulated in ox-LDL group (P < 0.05). A large number of red lipid droplets appeared in the cells in Vector group. Compared with Vector group, lipid droplets, the levels of TC, FC and CE and CE/TC, cholesterol efflux rate and expression of ABCA1 in RAW 264.7 macrophage was significantly decreased in LV-PRMT2 group (all P < 0.05). There was no significant difference about the expressions of ABCG1, CD36 and SR-A1 between LV-PRMT2 group and Vector group (all P > 0.05). CONCLUSIONS: Overexpression of PRMT2 inhibits the formation of foam cell induced by ox-LDL in RAW 264.7 macrophage, and the mechanism may be related to the increase of ABCA1 expression and ABCA1 mediated cholesterol efflux.


Subject(s)
ATP Binding Cassette Transporter 1/metabolism , Foam Cells/metabolism , Lipoproteins, LDL/metabolism , Protein-Arginine N-Methyltransferases/metabolism , ATP Binding Cassette Transporter 1/genetics , ATP Binding Cassette Transporter, Subfamily G, Member 1/metabolism , Animals , Arginine/metabolism , Atherosclerosis/metabolism , Biological Transport , CD36 Antigens/metabolism , Gene Expression Regulation , Lentivirus/genetics , Methylation , Mice , RAW 264.7 Cells , RNA, Messenger/metabolism , Receptors, Scavenger/metabolism , Transfection
4.
Mol Med Rep ; 11(3): 2141-6, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25411768

ABSTRACT

Renal cell carcinoma is the most frequent kidney malignancy and patients with metastatic disease have a poor prognosis. Suppressed apoptosis and marked invasiveness are distinctive features of renal cell carcinoma. In the present study, a dual­regulated oncolytic adenovirus expressing the interluekin (IL)­24 gene (Ki67­ZD55­IL­24) was constructed utilizing the Ki67 promoter to replace the native viral promoter of the E1A gene. Whether the combination of Ki67­ZD55­IL­24­mediated gene virotherapy and radiotherapy produced increased cytotoxicity in renal cell carcinoma cells via mitochondrial apoptotic cell death was investigated. The data indicated that this novel strategy has the potential to be further developed into an effective approach to treat renal cell carcinoma. The results showed that the combination of Ki67­ZD55­IL­24 and radiotherapy significantly enhanced anti­tumour activity via increasing the induction of apoptosis in melanoma cells compared with the other agents.


Subject(s)
Adenoviridae/genetics , Apoptosis/genetics , Genetic Vectors/genetics , Mitochondria/genetics , Oncolytic Viruses/genetics , Radiation Tolerance/genetics , Carcinoma, Renal Cell/genetics , Carcinoma, Renal Cell/radiotherapy , Cell Line, Tumor , Cell Survival/genetics , Cell Survival/radiation effects , Humans , Interleukins/genetics , Ki-67 Antigen/genetics , Promoter Regions, Genetic
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