ABSTRACT
OBJECTIVE: To investigate the intervention effect and mechanism of Aconite and Angelica sinensis on myocardial ischemia rats with Yang deficiency and blood stasis. METHODS: SPF-class SD rats were randomly divided into low-dose and high-dose groups. Each group was divided into control group, model group, and drug-administered group (FZ, DG, FG; 1 : 0.5, 1 : 1, 1 : 2). A rat model was prepared by intraperitoneal injection of hydrocortisone and isoproterenol plus cold stimulation. Each group was given corresponding decoction or distilled water for 14 days. The behavioral changes of rats in each group were observed. The morphological changes of rats cardiomyocytes were observed by HE staining. The average optical density (MOD value) and percentage of positive cells of Bcl-2, Bax, and Akt were determined by immunohistochemical staining method, and PEIs were calculated. Western blot and RT-PCR were used to determine the expression of PI3K, Caspase-3, Akt protein, and gene expression. RESULTS: The compatibility of Aconite and Angelica sinensis improved the morphology of rat cardiomyocytes, increased the PEI values of Akt and Bcl-2 protein, and decreased the PEI values of Bax protein (P < 0.01). The compatibility reduced the expression of Caspase-3 protein of rat myocardium and increased the protein expression of p-Akt, PI3K, and p-PI3K (P < 0.01). The compatibility also significantly reduced the expression of Caspase-3 mRNA and increased the expression of PI3K mRNA and Akt mRNA (P < 0.05 or P < 0.01), and the effect of high-dose FG (1 : 2) group is the best. CONCLUSIONS: The method of preparing a rat model of myocardial ischemia with Yang deficiency and blood stasis was feasible. The compatibility of Aconite and Angelica sinensis reduced myocardial fibrosis and inflammatory reaction, protected ischemic cardiomyocytes, and reduced myocardial injury, whose mechanism may be related to the regulation of PI3K/Akt pathway. The compatible group had better intervention effects than Aconite or Angelica sinensis alone. The best one was high-dose FG (1 : 2).
ABSTRACT
Understanding the impacts of climate change on runoff is of great importance for water resource assessments and adaptation strategy developments especially for the areas where scare and unevenly distributed water are available. Compared to the hydrological modelling method, the climate elasticity method is more flexible with the advantage of using few data in addressing the issue of investigating the effects of climate change on runoff. This study employed Budyko-based climate elasticity method, combined with temperature-based Blaney-Criddle equation, to obtain the elasticities of runoff to two major climate variables, and then applied this methodology to the upper reach of Heihe River basin, China. The runoff elasticity to precipitation in the study area was estimated to be 0.56-0.57, and the elasticity to temperature was -0.017 to -0.018. Precipitation increases showed a positive effect to runoff increases, and temperature increases showed a negative effect. Performances of 18 General Circulation Models (GCMs) of the Coupled Model Intercomparison Project Phase 5 (CMIP5) were assessed and the best GCMs were selected based on the entropy weighted TOPSIS approach. CSIRO-Mk3.6.0, CCSM4, and CanESM2 were ranked the first three with the best performances in simulating the observed precipitation and temperature over the study area. Climate projections from the above three GCMs showed that precipitation increased by 10% and 12% on average during the two periods of 2021-2050 and 2051-2080, producing 5.6% and 6.7% decreases in the projected long-term runoff compared to those in baseline period (1961-1990). Temperatures were projected to be increased by 2.0 °C and 2.9 °C for the two periods, resulting in the future long-term runoff decreased by nearly 2.0% and 2.9%, respectively.
