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Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
; 31(3): 254-60, 2005 Jun.
Article
in Chinese
| MEDLINE
| ID: mdl-15961899
ABSTRACT
Protoplasts of Swertia mussotii irradiated by ultra-violet light (UV) 260 microW/cm(2) were fused with protoplasts of Daucus carota var. sativa using the PEG method. Analysis of 5S rDNA spacer sequences and random amplified polymorphic DNA (RAPD) showed the presence and combination of nuclear DNA from both parents. All hybrids mainly contained genomes of D. carota (receptor) RAPD bands, and a few donor RAPD bands of S. mussotii. There is no obvious relationship between the nuclear DNA composition was not affected by UV-dosage. SSR analysis of chloroplast DNA of the hybrid clones further revealed that cpDNAs of the parents were integrated and recombined in the hybrids randomly.