ABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Fluorodeoxyglucose F18/administration & dosage , Lung Neoplasms/diagnostic imaging , Carcinoma/diagnostic imaging , Myoepithelioma/diagnostic imaging , Positron Emission Tomography Computed Tomography , Solitary Pulmonary Nodule/diagnostic imaging , Lymph Node Excision/methods , Lung Neoplasms/complications , Carcinoma/complications , Myoepithelioma/complications , Radiography, Thoracic , ImmunohistochemistryABSTRACT
OBJECTIVES: Form discordance of cavity walls (FDCW) and form concordance of cavity walls (FCCW) in multislice spiral CT (MSCT) were investigated to determine their value in differentiating between peripheral lung cancer cavities and single pulmonary tuberculous thick-walled cavities. An assessment of the role of multiplanar reconstruction (MPR) in detecting FDCW and FCCW was also performed. METHODS: MSCT cross-sectional images of 116 consecutive cases (including 60 cases with available MPR images) with peripheral lung cancer cavities and 118 consecutive cases (including 62 cases with available MPR images) with single pulmonary tuberculous thick-walled cavities (wall thickness >3 mm) were retrospectively analysed. According to the characteristics of cavitary internal and external walls on MSCT, these cavities were divided into two types (FDCW and FCCW). FDCW was further divided into three subtypes (FDCW-I, FDCW-II and FDCW-III); FCCW was further divided into two subtypes (FCCW-I and FCCW-II). RESULTS: On the cross-sectional and MPR images, the total detection rate of FDCW-I and FDCW-III in peripheral lung cancer cavities was 76.7% (89/116) and 93.3% (56/60), respectively, whereas the total detection rate of FCCW-I and FCCW-II in single pulmonary tuberculous thick-walled cavities was 75.4% (89/118) and 91.9% (57/62), respectively. CONCLUSIONS: FDCW-I, FDCW-III, FCCW-I and FCCW-II were valuable in differentiating between peripheral lung cancer cavities and single pulmonary tuberculous thick-walled cavities. MPR could improve the detection of FDCW-I and FDCW-III in peripheral lung cancer cavities and FCCW-I and FCCW-II in single pulmonary tuberculous thick-walled cavities.
Subject(s)
Carcinoma/diagnostic imaging , Image Processing, Computer-Assisted/methods , Lung Neoplasms/diagnostic imaging , Tuberculosis, Pulmonary/diagnostic imaging , Adult , Aged , Female , Humans , Male , Middle Aged , Multidetector Computed Tomography , Retrospective Studies , Tomography, Spiral Computed , Young AdultABSTRACT
BACKGROUND: C3H10T1/2 cells, from a mouse embryonic fibroblast cell line, were used to investigate the improvement of alginate-based microencapsulated cells for cellular therapy. METHODS: Purified sodium alginate (PSA) and non-purified sodium alginate (SA) were used to prepare alginate-based microcapsules, and their biocompatibility and membrane strength were then compared for the purposes of analyzing the advantages of purifying SA. In addition, poly-l-lysine (PLL) was replaced by chitosan for alginate-chitosan microcapsule preparation. The process of optimization and chemical modification of alginate-chitosan microcapsules using polyethylene glycol was also reviewed. RESULTS: The results showed improved biocompatibility and membrane strength of PSA-based microcapsules. Under optimal conditions, mesenchymal stromal cell (MSC)-loaded alginate-chitosan microcapsules with good morphology could be obtained using PSA and chitosans of medium molecular weight (1.0-2.5 x 10(5)). A chitosan solution of 0.1% (w/v) and a reaction time of 7 min between alginate and chitosan were determined as optimal preparation parameters. DISCUSSION: It could be concluded that the chemical modification of alginate-based microcapsules can improve their biocompatibility.
Subject(s)
Alginates/chemistry , Biocompatible Materials/chemistry , Capsules/chemistry , Stem Cell Transplantation/methods , Animals , Biocompatible Materials/pharmacology , Cell Culture Techniques , Cell Line , Cell Proliferation/drug effects , Cell Survival/drug effects , Chitosan/chemistry , Embryonic Stem Cells/chemistry , Embryonic Stem Cells/cytology , Fibroblasts/cytology , Glucuronic Acid/chemistry , Hexuronic Acids/chemistry , Membranes, Artificial , Mice , Polyethylene Glycols/chemistryABSTRACT
The EtOAc extract of Rabdosia coetsa showed angiotensin-converting enzyme (ACE) inhibitory activity. Bioassay-guided isolation of this extract yielded ethyl caffeate (1), rosmarinic acid (2) and methyl rosmarinate (3), which inhibited ACE activity by 32.42%, 55.19% and 39.50% respectively, at the concentration of 10 microg/ml.
Subject(s)
Angiotensin-Converting Enzyme Inhibitors/chemistry , Angiotensin-Converting Enzyme Inhibitors/pharmacology , Isodon/chemistry , Plant Extracts/pharmacology , Animals , Caffeic Acids/chemistry , Caffeic Acids/pharmacology , Cinnamates/chemistry , Cinnamates/pharmacology , Depsides/chemistry , Depsides/pharmacology , Lung/enzymology , Peptidyl-Dipeptidase A/drug effects , Peptidyl-Dipeptidase A/isolation & purification , Peptidyl-Dipeptidase A/metabolism , Plant Extracts/chemistry , Rabbits , Rosmarinic AcidABSTRACT
A novel alkaloid, gaultherialine A (1), along with twenty-seven known compounds were isolated from the whole plants of Gaultheria nummularioides D. Don. The structure of the new alkaloid was elucidated as 7,8-dimethoxy-1-(4-methoxyphenyl)-4,5-dihydro-2H-benzo[e]indole-2-one N-oxide (1) on the basis of spectral evidence.
Subject(s)
Alkaloids/isolation & purification , Gaultheria/chemistry , Alkaloids/chemistry , Molecular StructureABSTRACT
A new meroditerpenoid, igeumone (1), together with 18 known compounds (2-19), were isolated from ethanolic extract of the bark of Mayodendron igeum. Their structures were determined by analysis of spectral data or comparison with authentic samples. X-ray crystallographic analysis was employed to unambiguously determine the structure of 1.
Subject(s)
Bignoniaceae/chemistry , Diterpenes/isolation & purification , Trees/chemistry , China , Crystallography, X-Ray , Diterpenes/chemistry , Nuclear Magnetic Resonance, Biomolecular , Plant Bark/chemistry , Spectrometry, Mass, Electrospray Ionization , Spectrophotometry, Infrared , Spectroscopy, Fourier Transform InfraredABSTRACT
A novel tetrastilbenoid, flexuosol A (1), was isolated from the stems of Vitis flexuosa together with the known gnetin A, (+)-epsilon-viniferin, vitisin A, and hopeaphenol. The structure of 1 was elucidated using spectral methods.