ABSTRACT
A new ursane-type triterpenoid saponin, 2α,3α,24-trihydroxyurs-12,20(30)-dien-28-oic acid β-D-glucopyranosyl ester (1), together with six known triterpenoid saponins, was isolated and characterized from the aerial parts of Clematoclethra scandens subsp. actinidioides.
Subject(s)
Actinidiaceae , Chemistry , Molecular Structure , Plant Components, Aerial , Chemistry , Plant Extracts , Chemistry , Plants, Medicinal , Chemistry , Saponins , TriterpenesABSTRACT
A rapid and reliable method was established for simultaneous determination of main triterpenoids in Ganoderma lucidum spores using ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-TQ-MS). The established method was validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to determine the contents of 10 main triterpenoids in different batches of G. lucidum spores. The analysis results showed that moderate levels of triterpenoids were found in G. lucidum spores. In addition, a MS full scan with a daughter ion scan experiment was performed to identify the potential derivatives of triterpenoids present in G. lucidum spores. As a result, a total of 22 triterpenoids from different G. lucidum spores were unequivocally or tentatively identified via comparisons with authentic standards and literatures. This method provides both qualitative and quantitative results without the need for repetitive UPLC-MS analyses, thereby increasing efficiency and productivity, making it suitable for high-throughput applications.
Subject(s)
Chromatography, High Pressure Liquid/methods , Reishi/chemistry , Spores, Fungal/chemistry , Triterpenes/analysis , Chromatography, High Pressure Liquid/economics , Limit of Detection , Mass Spectrometry/economics , Mass Spectrometry/methods , Time FactorsABSTRACT
To study the chemical constituents of Clematoclethra scandens subsp. actinidioides, chromatographic methods such as silica gel and MCI column chromatographic technology, and preparative HPLC were used and sixteen compounds were isolated from the aerial parts of this plant. By using spectroscopic techniques including 1H, 13C-NMR, HMBC and ESI-MS, these compounds were identified as betulinic acid (1), ursolic acid (2), oleanic acid (3), corosolic acid (4), 3beta-(trans-p-coumaroyloxy)-2alpha, 23-dihydroxyurs-12-en-28-oic acid (5), 3beta-(trans-p-coumaroyloxy)-2alpha, 23-dihydroxyurs-12, 20 (30)-dien-28-oic acid (6), 2alpha, 3alpha, 23-trihydroxyurs-12, 20 (30)-dien-28-oic acid (7), 2alpha, 3alpha, 23-trihydroxyurs-12-en-28-oic acid (8), asiatic acid (9), 2alpha, 3alpha, 24-tri-hydroxyurs-12-en-28-oic acid (10), 2alpha, 3beta, 23-trihydroxyurs-12, 20 (30)-dien-28-oic acid (11), 2alpha, 3beta, 19alpha, 24-tetrahydroxyurs-12-en-28-oic acid (12), 2alpha, 3alpha, 19alpha, 24-tetrahydroxyurs-12-en-28-oic acid (13), 2alpha, 3beta, 23, 24-tetrahydroxyurs-12-en-28-oic acid (14), 2alpha, 3alpha, 19alpha, 23, 24-pentahydroxyurs-12-en-28-oic acid (15) and daucosterol (16). Among them, compounds 3-6, 11-12, 14 and 15 were isolated from this endemic plant for the first time.
Subject(s)
Actinidiaceae , Chemistry , Chromatography, Gel , Chromatography, High Pressure Liquid , Magnetic Resonance Spectroscopy , Plant Components, Aerial , Chemistry , Plant Extracts , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of Saussurea laniceps.</p><p><b>METHOD</b>The ethanol extract of S. laniceps was separated by means of silica gel chromatography. The compounds isolated from the plant were identified by their spectral evidence.</p><p><b>RESULT</b>Fifteen compounds were isolated and identified as beta-stiosterol (1), umbelliferone (2), 4-hydroxyacetophenone (3), scopoletin (4), isoscopoletin (5), xuelianlactone (6), methyl 3-(2', 4'-dihydroxyphenyl) propanoate (7), apigenin (8), neoechinulin A (9), daucosterol (10), scopolin (11), xuelianlactone 8-O-beta-D-glcuoside (12), apigenin 7-glcuoside (13), apigenin 7-lutinoside (14) and syringin (15).</p><p><b>CONCLUSION</b>Compounds 5-15 were isolated from S. laniceps, and among them, 7 and 9 were isolated from genus Saussurea for the first time.</p>
Subject(s)
Apigenin , Chemistry , Coumarins , Chemistry , Glucosides , Chemistry , Magnetic Resonance Spectroscopy , Phenylpropionates , Chemistry , Phorbols , Chemistry , Saussurea , Chemistry , Scopoletin , Chemistry , Sesquiterpenes , Chemistry , Umbelliferones , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of the whote plant Biebersteinia heterostemon (Geraniaceae).</p><p><b>METHOD</b>The ethanol extract of the whole plants was separated by various chromatographic methods and the compounds from the extract were identified by spectroscopic evidence including MS, IR, NMR and X-ray crystallographic analysis.</p><p><b>RESULT</b>Three isoprenyl guanidine derivatives were isolated from the whole plant of Biebersteinia heterostemon and identified as galegine (1) , cis-4-hydroxygalegine (2) and trans-4-hydroxygalegine (3).</p><p><b>CONCLUSION</b>The three compounds were isolated from this plant for the first time.</p>
Subject(s)
Geraniaceae , Chemistry , Guanidines , Chemistry , Molecular Conformation , Molecular Structure , Plant Bark , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Paederia scandense.</p><p><b>METHOD</b>The constituents were isolated and purified by silica gel and Sephadex LH - 20 column chromatography. Their structures were elucidated by physicochemical properties and spectral analysis.</p><p><b>RESULT</b>20 compounds were obtained and identified as rubiadin-1-methylether (1), diadzein (2), cleomiscosin B (3), cleomiscosin D (4), isolariciresinol (5), linarin (6), isoscopoletin (7), caffic acid (8), coumarinic acid (9), p-hydroxyl-benzoic acid (10), oleanolic acid (11), ursolic acid (12), beta-sitosterol (13), daucosterol (14), paederoside (15), paederosidic acid (16), paederosidic acid methyl ester (17), saprosmoside E (18), paederoscandoside (19), caffeic acid 4-O-beta-D-glucopyranoside (20).</p><p><b>CONCLUSION</b>Compounds 1-10, and 20 were isolated from this plant for the first time.</p>
Subject(s)
Coumarins , Chemistry , Isoflavones , Chemistry , Lignin , Chemistry , Naphthols , Chemistry , Plants, Medicinal , Chemistry , Rubiaceae , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents from the aerial parts of Hyperricum monogynum.</p><p><b>METHOD</b>Compounds were isolated by various column chromatography and identified by spectral analysis.</p><p><b>RESULT</b>Ten compounds were isolated and identified as quercetin, quercitrin, hyperoside, rutin, (-)-epicatechin, 3,5-dihydroxy-1-methoxy-xanthone, 3,4-O-isopropylidenyl shikimic acid, shikimic acid, daucosterol, and oleanoic acid.</p><p><b>CONCLUSION</b>All compounds were isolated from this plant for the first time.</p>
Subject(s)
Hypericum , Chemistry , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Quercetin , ChemistryABSTRACT
<p><b>AIM</b>To investigate the chemical constituents from the whole plant of the endemic plant Notoseris rhombiformis Shih.</p><p><b>METHODS</b>Column chromatography was used for separation and purification, while spectral analysis was used for structure elucidation.</p><p><b>RESULTS</b>Eight compounds were isolated from the whole plant of this Chinese endemic plant and their structures were elucidated as notoserolide A (1), notoserolide D (2), austricin (3), jacquilenin (4), 3 beta, 14-dihydroxy-11 beta, 13-dihydrocostunolide (5), p-hydroxyphenylacetic acid (6), luteolin-7-O-beta-D-glucopyranoside (7) and daucosterol (8), respectively.</p><p><b>CONCLUSION</b>All compounds were isolated from this plant for the first time. Among them, 2 is a new compound.</p>
