ABSTRACT
Covariant density functional theory and the tilted axis cranking method are used to investigate antimagnetic rotation (AMR) in nuclei for the first time in a fully self-consistent and microscopic way. The experimental spectrum as well as the B(E2) values of the recently observed AMR band in (105)Cd are reproduced very well. This gives a further strong hint that AMR is realized in specific bands in nuclei.
ABSTRACT
We conducted comparative proteomic analysis of osteosarcoma, with hopes of identifying the specific protein markers of osteosarcoma and improve the understanding of tumorigenesis and progression of osteosarcoma. Proteins extracted from osteosarcoma tissue and benign bone tumors, including osteoblastoma, chondroblastoma, and giant cell tumor of bone, were examined using two-dimensional gel electrophoresis followed by mass spectrometry analysis and database searches. We also validated the expression levels of interesting proteins by Western blotting assay and immunohistochemical staining. Intensity alterations of 30 spots were detected in osteosarcoma, and 18 of these spots were finally identified, including 12 up-regulated proteins and 6 down-regulated ones. The up-regulated proteins include VIM, TUBA1C, ZNF133, EZR, ACTG1, TF, and so on. The six down-regulated proteins include ADCY1, ATP5B, TUBB, RCN3, ACTB, and YWHAZ. Subsequent immunohistochemical staining and Western blotting assay for TUBA1C and ZNF133 in osteosarcoma samples confirmed the observation obtained by proteomic analysis. Our results suggest that these identified proteins may be potential biomarkers for osteosarcoma tumorigenesis and therapeutics. Aberrant expression of cytoskeletal- and microtubule-associated proteins in osteosarcoma may provide an advantage for tumor invasion and metastasis by affecting the stability of microtubule, which consequently influences the prognosis of patients.
Subject(s)
Bone Neoplasms/metabolism , Neoplasm Proteins/analysis , Osteoblastoma/metabolism , Osteosarcoma/metabolism , Proteomics/methods , Adolescent , Adult , Aged , Biomarkers, Tumor/analysis , Biomarkers, Tumor/metabolism , Bone Neoplasms/pathology , Bone and Bones/metabolism , Bone and Bones/pathology , Child , Chondroblastoma/metabolism , Chondroblastoma/pathology , Female , Giant Cell Tumor of Bone/metabolism , Giant Cell Tumor of Bone/pathology , Humans , Male , Middle Aged , Neoplasm Proteins/metabolism , Osteoblastoma/pathology , Osteosarcoma/pathology , Proteome/analysis , Proteome/metabolism , Young AdultABSTRACT
The list of authors in the paper by Zhi, Long, Chen & Ren [Acta Cryst. (2009), E65, o2008] is corrected and the acknowledgements are updated.[This corrects the article DOI: 10.1107/S1600536809028955.].
