Comparative evaluation of in vivo relative bioavailability and in vitro bioaccessibility of arsenic in leafy vegetables and its implication in human exposure assessment.

Arsenic (As) contamination in vegetables is a severe threat to human health. However, the evaluation of As relative bioavailability (As-RBA) or bioaccessibility in vegetables is still unexplored. The study sought to evaluate the As-RBA in commonly consumed ten leaf vegetables collected from As-polluted farmlands. Additionally, the As-RBA was determined using rat bioassay and compared with As bioaccessibility through five commonly used in vitro methods, including UBM (Unified BARGE Method), SBRC (Solubility Bioavailability Research Consortium), DIN (Deutsches Institut für Normung e.V.), IVG (In Vitro Gastrointestinal), and PBET (Physiologically Based Extraction Test). Results showed that the As-RBA values were 14.3-54.0% among different vegetables. Notably, significant in vivo-in vitro correlations (IVIVC) were observed between the As-RBA and the As bioaccessibility determined by the PBET assay (r2 = 0.763-0.847). However, the other assays (r2 = 0.417-0.788) showed a comparatively weaker relationship. The estimation of As-RBA using derived IVIVC to assess As exposure risk via vegetable consumption confirmed that As exposure risk based on As-RBA was lower than that the total As concentrations. Therefore, it was concluded that PBET could better predict the As-RBA in vegetables than other in vitro assays. Furthermore, As-RBA values should be considered for accurate health risk assessment of As in vegetables.

In Vivo-In Vitro Correlations for the Assessment of Cadmium Bioavailability in Vegetables.

The correlation of in vitro and in vivo assays for determining bioavailable Cd amounts in vegetables is limited. Herein, the correlations between Cd relative bioavailability (Cd-RBA) in rat models and Cd bioaccessibility in four in vitro assays were examined in vegetables. Results showed that the combined liver plus kidney data provided the appropriate endpoint and was used as a biomarker to estimate Cd-RBA. The Cd-RBA was negatively correlated with the mole ratio of Ca/Cd and Fe/Cd in vegetables. Strong in vivo-in vitro correlations were found from physiologically based extraction test (PBET) and in vitro gastrointestinal (IVG) (R2 = 0.66-0.69). We concluded that PBET and IVG were optimal models for Cd-RBA determination in vegetables. The nutritional elements in the vegetables could affect Cd absorption. Furthermore, the Cd bioavailability in vegetables should be considered because risk estimates solely based on the total Cd concentration in vegetables would overestimate Cd intake.