Effects of Replacing Fishmeal with Defatted Black Soldier Fly (Hermetia illucens Linnaeus) Larvae Meal in Japanese Eel (Anguilla japonica) Diet on Growth Performance, Fillet Texture, Serum Biochemical Parameters, and Intestinal Histomorphology.

An 8-week feeding trial was conducted to investigate the effects of replacing fishmeal with defatted black soldier fly larvae meal (DBSFLM) in the diets of Japanese eel on their growth performance, fillet texture, serum biochemical parameters, and intestinal histomorphology. Six isoproteic (520 g kg-1), isolipidic (80 g kg-1), and isoenergetic (15 MJ kg-1) diets were formulated with fishmeal replacement levels of 0% (R0), 15% (R15), 30% (R30), 45% (R45), 60% (R60), and 75% (R75). The growth performance, feed utilization efficiency, survival rate, serum liver function enzymes, antioxidant ability, and lysozyme activity of fish were not affected (P > 0.05) by DBSFLM. However, the crude protein and cohesiveness of the fillet in groups R60 and R75 significantly decreased, and the fillet hardness significantly increased (P < 0.05). Additionally, the intestinal villus length significantly decreased in the R75 group, and the goblet cell densities were significantly lower in the R45, R60, and R75 groups (P < 0.05). Overall, high levels of DBSFLM did not affect growth performance and serum biochemical parameters but significantly altered fillet proximate composition and texture and intestinal histomorphology (P < 0.05). The optimal fishmeal replacement level is 30% with 184 g kg-1 DBSFLM.

Using switchable solvent as a solvent and catalyst for in situ transesterification of spent coffee grounds for biodiesel synthesis.

This study developed a novel in situ transesterification process by combining the solvent and catalyst functions of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) for biodiesel synthesis from spent coffee grounds (SCG). The influence of reaction parameters on the reaction was investigated. The maximum biodiesel yield of 96.13% was observed at DBU-to-SCG and methanol-to-SCG ratios of 20 and 10 mL/g, respectively; a reaction temperature of 130 °C; and a reaction time of 60 min. Notably, the polarity of DBU could be reversibly regulated, thus facilitating excellent product separation. Moreover, DBU could be effectively reused for 10 cycles to yield high biodiesel conversion. DBU-catalyzed in situ transesterification of SCG is a promising, ecofriendly, and economically viable biodiesel production process.