ABSTRACT
Telocytes had been identified as a peculiar stromal cell type implicated in tissue homeostasis and the development and pathophysiology of diseases. Telocyte existed in most organs and tissues in humans and animals. However, few studies have examined telocytes in ApoE gene deficient mice. In our studies, we verified the existence, the morphology and immunohistochemical characteristics of telocytes in critical organs of the ApoE-/- mice. Male adult ApoE-/- mice were selected as an experimental model. Immunohistochemical bio-markers, such as CD34, CD117, CD28, Vimentin and PDGFR-α were utilized to determine the distribution and morphology of telocytes in the heart, liver and kidney. Telocyte expressed positively for CD34 and CD117, and partial telocyte and telopode expressed positively for PDGFR-α in heart and liver, but negatively in kidney. Double immunofluorescence assays for CD28/Vimentin, CD34/CD117 and CD34/PDGFR-α were used to demonstrate the biochemistry speciality of telocytes, respectively. The evidence of telocytes in the ApoE-/- mice is the first step of our sturdy, which aims to demonstrate changes in telocytes in atherosclerosis in this animal model.
Subject(s)
Biomarkers/metabolism , Kidney/cytology , Liver/cytology , Myocardium/cytology , Telocytes/cytology , Animals , Antigens, CD34/metabolism , CD28 Antigens/metabolism , Kidney/metabolism , Liver/metabolism , Male , Mice, Knockout, ApoE , Myocardium/metabolism , Proto-Oncogene Proteins c-kit/metabolism , Telocytes/metabolism , Vimentin/metabolismABSTRACT
Castleman disease (CD) is a rare disorder of lymph nodes and related tissues. CD generally occurs in the mediastinum, as well as in cervical, retroperitoneal and axillary regions. The disease is classified into two major types: unicentric CD (UCD) and multicentric CD. The occurrence of UCD in the retroperitoneal peripancreatic region is quite rare. We encountered two cases of retroperitoneal peripancreatic UCD in our hospital during the past three years. Following a series of medical examinations, including magnetic resonance imaging, computed tomography, ultrasonography and postoperative histopathological examination, these two patients were diagnosed with UCD, which presented as a retroperitoneal peripancreatic mass. The mass in each patient was completely excised, and no postoperative radiochemotherapy was administered. Both patients recovered well without recurrence during a follow-up period of 30 mo and 8 mo.
