Prednisone combined with Dihydroartemisinin attenuates systemic lupus erythematosus by regulating M1/M2 balance through the MAPK signaling pathway.

OBJECTIVE: Dihydroartemisinin (DHA) plays a very important role in various diseases. However, the precise involvement of DHA in systemic lupus erythematosus (SLE), relation to the equilibrium between M1 and M2 cells, remains uncertain. Therefore, we aimed to investigate the role of DHA in SLE and its effect on the M1/M2 cells balance. METHODS: SLE mice model was established by pristane induction. Flow cytometry was employed to measure the abundance of M1 and M2 cells within the peripheral blood of individuals diagnosed with SLE. The concentrations of various cytokines, namely TNF-α, IL-1ß, IL-4, IL-6, and IL-10, within the serum of SLE patients or SLE mice were assessed via ELISA. Immunofluorescence staining was utilized to detect the deposition of IgG and complement C3 in renal tissues of the mice. We conducted immunohistochemistry analysis to assess the expression levels of Collagen-I, a collagen protein, and α-SMA, a fibrosis marker protein, in the renal tissues of mice. Hematoxylin-eosin staining, Masson's trichrome staining, and Periodic acid Schiff staining were used to examine histological alterations. In this study, we employed qPCR and western blot techniques to assess the expression levels of key molecular markers, namely CD80 and CD86 for M1 cells, as well as CD206 and Arg-1 for M2 cells, within kidney tissue. Additionally, we investigated the involvement of the MAPK signaling pathway. The Venny 2.1 online software tool was employed to identify shared drug-disease targets, and subsequently, the Cytoscape 3.9.2 software was utilized to construct the "disease-target-ingredient" network diagram. Protein-protein interactions of the target proteins were analyzed using the String database, and the network proteins underwent enrichment analysis for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. RESULTS: The results showed that an increase in M1 cells and a decrease in M2 cells within the peripheral blood of individuals diagnosed with SLE. Further analysis revealed that prednisone (PDN) combined with DHA can alleviate kidney damage and regulate the balance of M1 and M2 cells in both glomerular mesangial cells (GMC) and kidney. The MAPK signaling pathway was found to be involved in SLE kidney damage and M1/M2 balance in the kidney. Furthermore, PDN and/or DHA were found to inhibit the MAPK signaling pathway in GMC and kidney. CONCLUSION: We demonstrated that PDN combined with DHA attenuates SLE by regulating M1/M2 balance through MAPK signaling pathway. These findings propose that the combination of PDN and DHA could serve as a promising therapeutic strategy for SLE, as it has the potential to mitigate kidney damage and reinstate the equilibrium of M1 and M2 cells.

Increased ACL direct insertion coverage provided more positive biomechanical effects on graft and bone tunnel during knee flexion: a simulation study.

PURPOSE: Flattened femoral tunnels were recently applied in anatomical single-bundle anterior cruciate ligament (ACL) reconstruction. Little is known about the biomechanical effect of such changes during knee flexion. The aim of the present simulation study was to assess the effect of altered ACL direct insertion coverage on the biomechanics of the graft and bone tunnel. METHODS: Five finite element (FE) models, including a round femoral tunnel and four progressively flattened rounded rectangular femoral tunnels, were established to represent the ACL reconstructions. In vivo knee kinematics data obtained from the validated dual fluoroscopic imaging techniques controlled the FE models to simulate lunge motions. The maximal principal stress of the graft and the volume of equivalent strain within 1000-3000 microstrain (V1000-3000) of the cancellous bone were subsequently calculated at 0°, 30°, 60° and 90° of knee flexion. RESULTS: A lower stress state on the graft and a more beneficial strain state on the cancellous bone were observed when the femoral tunnel better covered the ACL direct insertion. The average maximal principal stress of each model were 3.93 ± 0.60 MPa, 3.82 ± 0.54 MPa, 3.43 ± 0.44 MPa, 3.45 ± 0.44 MPa and 3.05 ± 0.43 MPa, respectively. The average V1000-3000 of the cancellous bone of each model were 179.06 ± 89.62 mm3, 221.40 ± 129.83 mm3, 247.57 ± 157.78 mm3, 282.74 ± 178.51 mm3 and 295.71 ± 162.59 mm3, respectively. Both the stress and strain values exhibited two peaks during the flexion simulation. The highest value occurred at 30° of flexion, and the second highest value occurred at 90° of flexion. CONCLUSIONS: Increased ACL direct insertion coverage provided more positive biomechanical effects after anatomical single-bundle ACL reconstruction during knee flexion.

Interaction between an Impurity and Nonlinear Excitations in a Polariton Condensate.

Exploring the dynamics of a mobile impurity immersed in field excitations is challenging, as it requires to account for the entanglement between the impurity and the surrounding excitations. To this end, the impurity's effective mass has to be considered as finite, rather than infinite. Here, we theoretically investigate the interaction between a finite-mass impurity and a dissipative soliton representing nonlinear excitations in the polariton Bose-Einstein condensate (BEC). Using the Lagrange variational method and the open-dissipative Gross-Pitaevskii equation, we analytically derive the interaction phase diagram between the impurity and a dissipative bright soliton in the polariton BEC. Depending on the impurity mass, we find the dissipative soliton colliding with the impurity can transmit through, get trapped, or be reflected. This work opens a new perspective in understanding the impurity dynamics when immersed in field excitations, as well as potential applications in information processing with polariton solitons.

The non-Hermitian geometrical property of 1D Lieb lattice under Majorana's stellar representation.

The topological properties of non-Hermitian Hamiltonian is a hot topic, and the theoretical studies along this research line are usually based on the two-level non-Hermitian Hamiltonian (or, equivalently, a spin-1/2 non-Hermitian Hamiltonian). We are motivated to study the geometrical phases of a three-level Lieb lattice model (or, equivalently, a spin-1 non-Hermitian Hamiltonian) with the flat band in the context of a polariton condensate. The topological invariants are calculated by both winding numbers in the Brillouin zone and the geometrical phase of Majorana stars on the Bloch sphere. Besides, we provide an intuitive way to study the topological phase transformation with the higher spin, and the flat band offers a platform to define the topological phase transition on the Bloch sphere. According to the trajectories of the Majorana stars, we calculate the geometrical phases of the Majorana stars. We study the Lieb lattice with a complex hopping and find their phases have a jump when the parameters change from the trivial phase to the topological phase. The correlation phase of Majorana stars will rise along with the increase of the imaginary parts of the hopping energy. Besides, we also study the Lieb lattice with different intracell hopping and calculate the geometrical phases of the model using non-Bloch factor under the Majorana's stellar representation. In this case, the correlation phases will always be zero because of the normalized coefficient is always a purely real number and the phase transition is vividly shown with the geometrical phases of the Majorana stars calculated by the mean values of the total phases of both right and the joint left eigenstates.

Beliaev Damping of a Spin-Orbit-Coupled Bose-Einstein Condensate.

Beliaev damping provides a fundamental mechanism for dissipation of quasiparticles. Previous research has shown that the two-component internal degrees of freedom has no nontrivial effect on Beliaev damping. Here we provide the first example where the spinor nature of Bose gases can manifest itself in the Beliaev damping by way of spin-obit coupling. We identify novel features of the Beliaev decay rate due to spin-orbit coupling; in particular, it shows an explicit dependence on the spin-density interaction and diverges at the interaction-modified phase boundary between the zero-momentum and plane wave phases. This represents a manifestation of the effect of spin-orbit coupling in the beyond-mean-field regime, which by breaking Galilean invariance couples excitations in the density and spin channels. We further show that the measurement of the Beliaev damping rate is experimentally feasible through the measurement of spin polarizability susceptibility, which has been already achieved in spin-orbit-coupled Bose gases.

Visualization of dimensional effects in collective excitations of optically trapped quasi-two-dimensional Bose gases.

In quasi-two dimensions (quasi-2D), where excitations are frozen in one direction, the scattering amplitudes exhibit 2D features of the particle motion and a 3D to 2D dimensional crossover emerges in the behavior of scattering. We explore its physical consequences, capitalizing on a hidden connection between the Pitaevskii-Rosch dynamical symmetry and breathing modes. We find broken Pitaevskii-Rosch symmetry by arbitrarily small 2D effects, inducing a frequency shift in breathing modes. The predicted shift rises significantly from the order of 0.5% to more than 5% in transiting from the 3D-scattering to the 2D-scattering regime. Comparisons with other relevant effects suggest our results are observable within current experimental capabilities.