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Mol Biol (Mosk) ; 51(4): 629-636, 2017.
Article in Russian | MEDLINE | ID: mdl-28900081

ABSTRACT

MicroRNA-218 (miR-218) is a short, noncoding RNA, with multiple biological functions. In this study, we aimed to investigate the potential effects of miR-218 on the apoptosis of human ovarian carcinoma cells and the underlying mechanisms by which miR-218 exerted its actions. After over-expressing miR-218 in human ovarian carcinoma (OVCAR3) cells, cell viability was determined by MTT method, cell apoptosis was observed by flow cytometry (FCM), mRNA expression of miR-218, Bcl2, Bax was measured by RT-PCR and protein expression levels of Wnt, tankyrase and ß-catenin were quantified by Western blots. Over-expression of miR-218 potently suppressed cell viability and promoted the apoptosis of human ovarian carcinoma cells in a time-dependent manner. In addition, the down-regulation of tankyrase expression level was detected in miR-218-over-expressed cells. Following the block of the Wnt/ß-catenin signaling pathway using the inhibitor XAV-939, the effects of miR-218 on the proliferation and apoptosis of human ovarian carcinoma cells were significantly suppressed. Augmenting expression of miR-218 and/or miRNA-218 mimicking therapeutics may provide viable avenue for the treatment of ovarian cancer.


Subject(s)
Epithelial Cells/metabolism , Gene Expression Regulation, Neoplastic , MicroRNAs/genetics , Wnt Proteins/genetics , beta Catenin/genetics , Apoptosis/genetics , Cell Line, Tumor , Cell Survival , Epithelial Cells/drug effects , Epithelial Cells/pathology , Female , Heterocyclic Compounds, 3-Ring/pharmacology , Humans , MicroRNAs/metabolism , Ovary/drug effects , Ovary/metabolism , Ovary/pathology , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , Tankyrases/genetics , Tankyrases/metabolism , Wnt Proteins/antagonists & inhibitors , Wnt Proteins/metabolism , Wnt Signaling Pathway , bcl-2-Associated X Protein/genetics , bcl-2-Associated X Protein/metabolism , beta Catenin/antagonists & inhibitors , beta Catenin/metabolism
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