ABSTRACT
OBJECTIVE: To preliminarily understand the genotyping characteristics regarding the variable number tandem repeats (VNTR) of Mycobacterium tuberculosis clinical isolates so as to provide evidence for the development of tuberculosis control and prevention programs in Fujian province. METHODS: Fifteen VNTR locus sets were used to detect the clinical isolates from the fifth surveillance project on tuberculosis resistance, in Fujian province. BioNumerics version 4.5 were used to analyze the cluster from the results generated by genotyping. RESULTS: 313 Mycobacterium tuberculosis isolates were divided into 9 clusters, including I, II, III, IV, V, VI, VII, VIII and IX, with the number of 220, 9, 48, 2, 1, 3, 10, 10, 10 isolates, respectively. Cluster I was the major lineage, accounting for 70.3% (220/313) of the total. Resistance rates of cluster I isolates to isoniazid, streptomycin, ethambutol and multi-drug-resistant were not statistically different from other clusters (P > 0.05). However, resistance rate to rifampicin (RFP) was significantly higher than that of other isolates of the clusters, 33.2% (73/220) vs. 20.4% (19/93) (P < 0.05). CONCLUSION: The strains isolated from Fujian province showed significant polymorphism on genotyping. Cluster I seemed to be the dominant, calling for the close monitoring program on cluster I strains. RESULTS: from our initial studies demonstrated the existence of significant correlation between cluster I strains and drug resistance to RFP.
Subject(s)
Minisatellite Repeats , Mycobacterium tuberculosis/genetics , Adolescent , Adult , Aged , Aged, 80 and over , China , Female , Genotype , Humans , Male , Middle Aged , Mycobacterium tuberculosis/isolation & purification , Young AdultSubject(s)
Tuberculosis/epidemiology , Adolescent , Child , Child, Preschool , China/epidemiology , Humans , Incidence , Infant , Mycobacterium bovis , Tuberculosis/prevention & control , VaccinationABSTRACT
<p><b>AIM</b>To assess the effect of gender on genetic polymorphism of cytochrome CYP2C19 in Chinese population.</p><p><b>METHODS</b>The genetic polymorphism of 140 healthy Chinese were analysed by PCR-RFLP (restriction fragment length polymorphism).</p><p><b>RESULTS</b>Of 52 genotyped male subjects, 23 (44.23%) were homozygous for wildtype (wt/wt), 6 (11.54%) were homozygous for CYP2C19 m1 (m1/m1), and 23 (44.23%) were heterozygous for CYP2C19 m1 or CYP2C19 m2 (wt/m1 or wt/m2); and among the 88 genotyped female subjects, 31 (35.23%) were homozygous for wildtype (wt/wt), 13 (14.82%) were homozygous for CYP2C19 m1 (m1/m1), and 44 (50.0%) were heterozygous for CYP2C19 m1 or CYP2C19 m2 (wt/m1 or wt/m2); no homozygous genotype for CYP2C19 m2 (m2/m2) was found in the study.</p><p><b>CONCLUSION</b>There is no statistical difference in ocurance of wt/wt and m1/m1 between in male and in female, so gender have no significant effect on genetic polymorphism of cytochrome CYP2C19.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Aryl Hydrocarbon Hydroxylases , Genetics , Asian People , China , Cytochrome P-450 CYP2C19 , Gene Frequency , Genotype , Mixed Function Oxygenases , Genetics , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Sex FactorsABSTRACT
AIM: To set up a method which can determining the blood concentration of omeprazole and its metabolite 5′-omeprazole, omerpazole sulphone in order to study its cinical pharmacokinetics. METHODS: The blood concentration of omerpazole was determinated by HPLC. RESULTS: Calibrated standard curve of omeprazole in blood is Y = -0.004 499 + 0.001 909X (r = 0.9990), the recoveries of three concentrations 50, 500, 2 000 mg·L-1 are 90.36, 109.62, 108.91%, respectively; and the precisions are 9.86, 7.86, 15.52%, respectively. Calibrated standard curve of its metabolite 5′-OH omeprazole in plasma is Y = - 0. 003 659 + 0. 001 328X (r = 0.9970), the recoveries of three concentrations 20, 200, 1 000 mg·L-1 are 79.42%, 90.49 %, 93.04%, respectively; and the precisions are 8.95%, 4.52%, 9.73%, respectively. Calibrated standard curve of its another metabolite omeprazole sulphone in plasma is Y = 0.009 248 + 0.001 765X (r = 0. 999 2), the recoveries of three concentrations 20, 200, 1 000 mg·L-1 are 94.44%, 105.59%, 104.26%, respectively; and the precisions are 8.72, 8.58, 9.60%, respectively. After 20 mg omeprazole were administered by a voluteer via oral, Cmax of 5′-OH omeprazole, omeprazole, and omerpazole sulphone were 14.622 7, 408.433 2, 454.363 7 mg·L-1. CONCLUSION: The method is good enough to study pharmacokinetics of omeprazole.
