ABSTRACT
Osteoarthritis (OA) is the most prevalent degenerative joint disease worldwide. Effective management for early-stage OA is crucial. Denosumab (DS) has been widely used to treat osteoporosis (OP) and rheumatoid arthritis, but its potential for managing OA remains clear. We assessed the effects of DS on osteoclast activity and chondrocyte apoptosis using tartrate-resistant acid phosphatase (TRAP) assay, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry, and TUNEL staining. To assess the impact of DS on the NF-κB pathway, we performed Western blot and immunofluorescence staining. Additionally, we used an OA model to explore the influence of DS on subchondral bone remodeling and cartilage degeneration in vivo. We found that DS hindered receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclastogenesis by inhibiting the activity of the NF-κB pathway. Besides, DS alleviated reactive oxygen species (ROS)-induced apoptosis in chondrocytes by regulating the expression of genes related to apoptosis. Moreover, we observed an attenuation of OA-related subchondral bone remodeling and cartilage degeneration in vivo. Our findings indicate that DS could effectively suppress osteoclast activity and chondrocyte apoptosis, thereby mitigating OA-related subchondral bone remodeling and cartilage degeneration. These results provide a mechanistic basis for using DS to treat OA.
ABSTRACT
Osteoarthritis (OA) mainly occurs in the elderly population and seriously affects their quality of life (QOL). Strontium (Sr) ions have shown positive effects on bone tissue and are promising for OA treatment. However, the adequate treatment dosage and underlying mechanisms are unclear. This study investigated the effects and underlying mechanisms of different concentrations of Sr ions in a mouse model of OA induced by destabilization of the medial meniscus (DMM) surgery. DMM-induced OA mice received intra-articular injections of different concentrations of Sr ions, and a suitable concentration of Sr ions was found to improve OA. Furthermore, we investigated the mechanism by which Sr ions mediate senescence and autophagy in fibroblast-like synoviocytes (FLSs) in the synovial tissues of DMM-induced OA mice. OA mice treated with 10 µl of 5 mmol/L SrCl2 showed the greatest improvement in pain-related behavior and cartilage damage. In addition, in vivo and in vitro experiments revealed that Sr ions inhibit senescence and improve the autophagic function of FLSs. We also found that enhancement of the autophagic function of FLSs could effectively slow down senescence. Therefore, we show that Sr ions through the AMPK/mTOR/LC3B-II signal axis improve FLSs autophagy function and delay FLSs senescence, and furthermore, improve OA. These results suggest that senescence and autophagy function of FLSs may serve as promising targets for OA treatment, and that Sr ions may inhibit OA progression through these two targets.
Subject(s)
Osteoarthritis , Synoviocytes , Aged , Humans , Mice , Animals , Quality of Life , Osteoarthritis/drug therapy , Autophagy , FibroblastsABSTRACT
More than 100 surgical procedures have been reported to address recurrent patellar instability. Trochlear dysplasia is the most common finding among the anatomic risk factors for recurrent patellar instability. Various studies have shown that trochleoplasty combined with medial patellofemoral ligament reconstruction is an effective technique to treat recurrent patellar instability. Nevertheless, trochleoplasty is still a daunting procedure for surgeons because of its multiple and gloomy complications. This article introduces a technique to treat recurrent patellar instability with severe trochlear dysplasia: precise arthroscopic mini-trochleoplasty (PAM trochleoplasty) combined with medial patellofemoral ligament reconstruction. This technique precisely removes the supratrochlear spur and trochlear bump, accurately reshapes the trochlear sulcus with minimal invasion and less osteotomy volume, and keeps the sulcus cartilage intact. The purpose of this technique is to develop trochleoplasty into a common and safe technique, which has good outcomes and low complications.
ABSTRACT
Steam on the camera may happen in any arthroscopic procedure, especially in shoulder arthroscopy. Although multiple techniques and devices have been applied to avoid steam, it still cannot be prevented completely. We designed a simple device to terminate this problem by protecting against the ingress of water.
