ABSTRACT
OBJECTIVE: To establish analysis methods for fingerprint of Kanggongyan series by HPLC. METHODS: A Shiseido CAP-CELL PAK C18(250 mm x 4. 6 mm, 3 µm) column was used with acetonitrile-0. 5% phosphoric acid as the mobile phase by gradient elution. The flow rate was 0. 8 mL/min, the column temperature was 30 °C, and the detection wavelength was set at 280 nm during 0 ~ 44 min and at 332 nm during 44 ~ 115 min. RESULTS: Ten common peaks were selected as characteristic peaks in the chromatogram of Kanggongyan particles, eleven common peaks were selected as characteristic peaks in the chromatogram of Kanggongyan tablets and capsules ,the similarities were greater than 0. 9 among all batches. CONCLUSION: The method is simple, steady and repeatable. It provides a basis for the quality control of Kanggongyan series.
