[Study on preparaion of nongsuo danggui dispersible tablets and determination of its dissolution].

OBJECTIVE: To prepare nongsuo danggui dispersible tablets and determine its dissolution. METHODS: The dissolution of the tablets was determined by HPLC. RESULTS: The linear range of ferulic acid was 0.0557 - 0.557 microg (r = 0.9998). The average recoveries met the requirement. Phosphate solution of pH 6. 8 was the dissolution medium. CONCLUSION: The method is simple, accurate and has stronge specificity and can be used for the quality control of nongsuo danggui dissolveing tablets.

[Analysis of volatile oil from Gerbera piloselloides by GC-MS].

OBJECTIVE: To identify the chemical components of volatile oil from Gerbera piloselloides. METHODS: Volatile oil was extracted from Gerbera piloselloides by steam distillation, and its chemical components were identified by GC-MS. RESULTS: 22 peaks were detected, among which 17 compounds were identified, accounting for 86.18% of total peak area. CONCLUSION: In the total volatile oil from Gerbera piloselloides, Neryl(s)-2-methylbutanoate (35.99%), 4-hydroxy-3-methylacetophenone (8.74%) and n-Hexadecanoic acid (7.48%) are the main components.

[Determination of oleanolic acid in fufang zhenqin feining capsules by RP-HPLC].

OBJECTIVE: To investigate the optimal technology of extraction of fufang zhenqin feining capsules and establish a method for determination its oleanolic acid. METHODS: Orthogonal test was employed for selecting the optimum extraction technology by the index of the content of oleanolic acid in extraction. HPLC was performed on a C18 (250 mm x 4.6 mm, 5 microm) column using methanol-0.3% triethylamine solution (80: 20) as the mobile phase at a flow rate of 1 ml/min. The detection wavelength was set at 210 nm. The column temperature was 30 degrees C and the injection size was 25 microL. RESULTS: The optimum extraction technology was as follows: the concertration of alcohol was 70%, the ratio of solid/material was 8: 1, extracting 3 times with half an hour for each time. The linear range of oleanolic acid was 59.68 - 596.8 microg/ml (r = 0.9997), the average recovery was 99.79% (RSD = 0.5%). CONCLUSION: The considerable extraction rate of active components in the drugs is achieved by applying the selected technology, and the simple method is fit for production. The established HPLC method is simple, accurate, special and can be used for the quality control of fufang zhenqin feining capsules.

[HPLC fingerprint study on Chaenomelis fructus and comparative study on different processed products].

OBJECTIVE: To establish HPLC fingerprint of Chaenomelis Fructus and compare HPLC fingerprints of different processed products. METHODS: The mobile phase consisted of acetonitrile-methanol (7:3) (A) and 0.03% phosphoric acid(B) in a gradient mode. Flow rate was 0.8 mL/min. The wavelength was set at 300 nm and column temperature was 30 degrees C. The HPLC chromatograms were analyzed by the "traditional Chinese medicine fingerprint similarity calculation software". RESULTS: The results of methodological study met technical requirements of HPLC fingerprint. Ten batches of samples were divided into three groups by hierarchical cluster analysis and the similarities exceeded 0.90. The HPLC fingerprints of different processed products had obvious differences in area of common peaks but less differences in number of common peaks. CONCLUSION: The HPLC fingerprint method is stable, accurate and reliable and can be used for scientific quality control of Chaenomelis Fructus. The processing temperature and processing materials have effect on the dissolution of chemical compositions in Chaenomelis Fructus.

[Determination of vitexin in plasma by HPLC-MS/MS method and its pharmacokinetics in rats].

OBJECTIVE: To establish a HPLC-MS/MS method for the determination of vitexin in rat plasma and its pharmacokinetics. METHODS: The HPLC-MS/MS method used Capcell Pak C18 column (50 mm x 2.0 mm I. D., 5 microm). The mobile phase was methanol and water (95:5, V/V, containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Electrospray ionization (ESI) in negative ion mode and multiple reaction monitoring (MRM) was used for the quantification of vitexin with a monitored transitions m/z 431-->311 for vitexin and m/z 269-->225 for internal standard (I. S., emodin). RESULTS: Linear calibration curves were obtained over the concentration range of 0.5-2000 ng/mL (r = 0.9960) with the lowest limit of quantification (LLOQ) of 0.5 ng/mL. The recovery was in the range of 76.1%-89.0%. The relative standard deviations for the intra-day and inter-day validation were less than 11%. CONCLUSION: The method is simple, accurate, fast, sensitive and suitable for the pharmacokinetic study of vitexin in rats.

[Study on the extraction process of total flavonoids from Psoralea corylifolia by enzyme-assisted technique].

OBJECTIVE: To study the optimum extraction process of total flavonoids from Psoralea coryl folia by cellulose-assisted technique. METHODS: Based on single-factor experiments, the effects of pH value, temperature of enzymatic hydrolysis, time of enzymatic hydrolysis and enzyme amount on the extraction yields of total flavonoids from Psoralea corylifolia were studied by response surface methodology. RESULTS: The optimum enzyme-assisted extraction process was: pH value 4.9, temperature 46 degrees C, time 150 min and enzyme amount 7.2 mg/g,under this condition,the relative error of the observed and predicted values was 1.16%. CONCLUSION: The optimum enzyme-assisted extraction process is simple and feasible, the extraction rate of total flavonoids increases by 28% compared with ultrasonic extraction, so it can be used to extract total flavonoids from Psoralea corylifolia.

[Preparation and examination of the vitro releasing of pH-dependent kuijiekang tablet for colon-specific delivery].

OBJECTIVE: To prepare the pH-dependent kuijiekang tablet for colon-specific delivery used in treating the ulcerative colitis and to develop the in vitro release method of kuijiekang tablets. METHODS: The coating prescription was screened by the in vitro delivery of Puerarin and Berberine hydrochloride. The in-vitro releasing property of the preparation was examined by the method of in-vitro delivery. RESULTS: The preparation methods of the pH-dependent kuijiekang tablet for colon-specific delivery was obtained from the in-vitro delivery, Puerarin and Berberine hydrochloride were not detected in the simulated gastric fluid after 2 h and the quantitied of Puerarin and Berberine hydrochlo4de were less 10% in the simulated intestinal fluid after 4 h. The quantities of Puerarin and Berberine hydrochloride were 90.07% and 89.87%. CONCLUSION: It can be prepared and the preparation is a promising delivery system for drugs to be delivery to the colon.

[Determination of tanshinone II A and deoxyschizandrin in danshenwuweizi tablet by RP-HPLC].

OBJECTIVE: to set up a determination of tanshinone II A and deoxyschizaadrin in danshenwuweizi tablet by RP-HPLC. METHODS: two components were separated through Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with methanol-water (85:15) by volume as a mobile phase. The flow rate was 1 ml x min(-1) and the detection wavelength were 270 nm (tanshinone II A) and 254 nm (deoxyschizandrin). RESULTS: The linear range of tanshinone II A and deoxyschizandrin were 0.3232 microg-1.616 microg and 0.525 microg-2.625 microg, respectively. The average recovery was 99.83% with RSD 0.36% for tanshinone II A and 100.2% with RSD 0.72% for deoxyschizandrin respectively. CONCLUSION: The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of danshenwuweizi tablet.

[Studies on the chemical constituents of essential oil of Hunan Artemisia annua].

The chemical constituents of the essential oil of Hunan wild Artemisia annua L. in Xuefeng mountain region have been studied by means of the GC-MS. The essential oil was extracted from Artemisia annua L. by steam distillation. The chemical constituents were separated and identified by GC-MS, and the relative contents of them in the oil were determined with area normalization method. Forty-five components were separated and identified which accounted for 90% of total essential oil. These components identified from the essential oil indicate that Hunan wild Artemisia annua L. in Xuefeng mountain region is a good medical plant, and worth to exploiting.