ABSTRACT
Previously, we determined that the CARD11 rs4722404 single nucleotide polymorphism (SNP) increases risk of early-onset psoriasis vulgaris (PsV). Moreover, the CARD14 gene polymorphism c.C2458T (p.Arg820Trp) is associated with clinical features of this disease. CARMA1/CARD11, CARMA2/CARD14, and CARMA3/CARD10 are conserved across many species and constitute a family of proteins, all of the members of which contain various functional domains characteristic of this group. The NF-κB signaling pathway, regulated by the CARMA family of scaffold proteins and its eponymous component, is a crucial mediator in the pathogenesis of psoriasis. However, little is known about the association between CARMA3/CARD10 and PsV. The aim of this study was to evaluate the relationship between the gene encoding this protein and risk of PsV in the southern Han Chinese population. Genomic DNA from 568 individuals of southern Chinese origin, including 355 patients with PsV and 213 control subjects, was analyzed. We selected seven tag SNPs in the CARMA3/CARD10 gene and genotyped them by the SNaPshot assay. Our results identified no significant association between these SNPs and PsV in the Chinese population examined. Future studies should focus on the potential function of the CARMA3/CARD10 gene in the pathogenesis of PsV.
Subject(s)
CARD Signaling Adaptor Proteins/genetics , Adult , Asian People/genetics , Case-Control Studies , China , Female , Gene Frequency , Genetic Association Studies , Genetic Predisposition to Disease , Humans , Male , Middle Aged , Polymorphism, Single Nucleotide , Psoriasis , Risk , Sequence Analysis, DNAABSTRACT
Para o conhecimento do estado de poluicao bacteriologica dos rios Anil e Bacanga, Estado do Maranhao (Brasil), coletaram-se amostras de agua em 5 pontos, no Rio Anil, e 3 pontos, no Rio Bacanga, mensalmente, durante 12 meses, nas fases enchente e vazante da mare. Foram determinados numeros de bacterias totais/ml; indice de coliformes/100ml e indice coliformes fecais/100ml em agar simples, caldo lactosado, verde brilhante - bile e E. C. Os resultados mostraram que os indices bacterianos de poluicao dos rios sofrem variacoes sazonais, diretamente relacionadas as fases de mare. As localidades que apresentaram maiores concentracoes bacterianas foram as nascentes. As aguas destes rios, de acordo com o padrao ditado pelo Ministerio do Interior, demonstraram alto grau de poluicao microbiana de origem fecal