ABSTRACT
OBJECTIVE: To examine the pathological changes in the liver of an AIDS patient with complicated infection of Pneumocystis carinii(PC). METHODS: A liver biopsy was made. The tissue was stained with HE, PAS, Giemsa, GMS, and acid-fast staining, and examined under light microscope and transmission electron microscope. RESULTS: Granulomas (acid-fast negative) in the tissue and numerous pathogens (PAS positive) in hepatic sinusoids were detected. Giemsa and GMS staining and electron microscopy all confirmed that the pathogen was Pneumocystis carinii. CONCLUSION: The pathological findings revealed a diffuse extrapulmonary infection of Pneumocystis carinii in the patient of AIDS.
Subject(s)
Acquired Immunodeficiency Syndrome/complications , Liver Diseases/pathology , Liver/pathology , Pneumocystis Infections/pathology , Acquired Immunodeficiency Syndrome/pathology , Adult , Biopsy , Humans , Liver Diseases/complications , Liver Diseases/microbiology , Male , Microscopy, Electron , Pneumocystis Infections/complications , Staining and LabelingABSTRACT
This report describes isolation of virus particles from plants of rice, maize, wheat and sorghum with symptoms of dwarfing collected from two provinces of China, purification of double-stranded RNA (dsRNA) from the virus particles, and synthesis of full-length cDNAs of genome segments 9 (S9) and 10 (S10) by reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis showed that the S9 sequences of the Chinese isolates and a Japanese rice black-streaked disease virus (RBSDV) isolate were very similar (89.1-89.6% homology at nucleotide level and 92.3-92.9% and 95.8-98.6% homology at amino acid level for ORF1 and ORF2, respectively). Analogical similarity was found also for the S10 sequences of the isolates under comparison: 93.0-95.4% homology at nucleotide level and 96.2-97.0% homology at amino acid level. However, there was a relatively lower similarity for S9 and S10 segments ofthe Chinese isolates and an Italian maize rough dwarf virus (MRDV) isolate. The phylogenetic analysis indicated that the Chinese isolates that infect rice, maize, wheat and sorghum and cause similar symptoms could represent the same virus species, RBSDV.
Subject(s)
Edible Grain/virology , Plant Diseases/virology , RNA, Double-Stranded/analysis , Reoviridae/isolation & purification , Amino Acid Sequence , China , Edible Grain/classification , Edible Grain/growth & development , Molecular Sequence Data , Open Reading Frames , Phylogeny , RNA, Double-Stranded/genetics , RNA, Viral/analysis , RNA, Viral/genetics , Reoviridae/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNAABSTRACT
An immunosuppressed rat model was established by injecting cortisone acetate 25 mg/rat twice a week for 4 weeks and 12.5 mg/rat for another 2 weeks subcutaneously. A development of Pneumocystis carinii pneumonia (PCP) was found at the end of the 6th week in all rats. These rats were injected intramuscularly with artemether at 100 mg/kg once a day for 5 consecutive days. All rats were necropsied at the end of the 8th week. The lung impression smears were stained with Gomori's stain, and Pneumocystis carinii cysts were counted. The ultrastructural changes of trophozoite, precyst and cyst were investigated by transmission electron microscopy on the 7th day after treatment.
