Influence of Chrysanthemum morifolium-maize intercropping pattern on yield, quality, soil condition, and rhizosphere soil microbial communities of C. morifolium.

Introduction: Chrysanthemum morifolium Ramat. is a perennial herb in the Compositae family, often employed in traditional Chinese medicine due to its medicinal value. The planting of C. morifolium faces the challenges of continuous cropping, and intercropping is able to somewhat overcome the obstacles of continuous cropping. Methods: In our study, we designed two different C. morifolium-maize intercropping patterns, including C. morifolium-maize narrow-wide row planting (IS) and C. morifolium-maize middle row planting (IM). Compared with monoculture, the agronomic traits, yield, active ingredients, soil physicochemical properties, soil enzyme activities, and rhizosphere soil microbial communities of C. morifolium and maize were measured under the two C. morifolium-maize intercropping patterns. Results: The findings indicated that (1) Intercropping elevated the agronomic traits, yield, and active ingredients of C. morifolium, especially in C. morifolium-maize narrow-wide row planting pattern, which indicating that interspecific distance played an important role in intercropping system; (2) Intercropping enhanced soil physicochemical properties and enzyme activities of C. morifolium and maize; (3) Intercropping altered rhizosphere soil microbial communities of C. morifolium and maize, making microbial interrelationships more complex. (4) Intercropping could recruit a large number of beneficial microorganisms enrich in the soil, including Bacillus, Sphingomonas, Burkholderia-Caballeronia-Paraburkholderia, Chaetomium, and Ceratorhiza, which may increase the content of AN, NN, AvK, ExCa, AvCu, AvZn and other nutrients in soil and promoted the growth and quality of C. morifolium. Discussion: In summary, intercropping with maize could promote the accumulation of beneficial microorganisms in the soil, thus improving the overall growing environment, and finally realizing the growth and improvement of C. morifolium.

Optical imaging system for an all-time star sensor based on field of view gated technology.

The wide field of view (FOV) of traditional star sensor optical systems restricts the ability to suppress atmospheric background. An optical imaging system for an all-time star sensor based on FOV gated technology is proposed. In this system, a wide FOV telescope is used to observe a large sky area containing multiple stars. A microlens and microshutter array is employed to subdivide the wide FOV and gate a narrow FOV to suppress atmospheric background radiation. Assisted by a common imaging lens, each set of microlens and microshutter elements corresponds to a FOV gated imaging channel. With the rapid switching of gated FOV, multiple stellar images are obtained on a common detection during daytime. As an example, a FOV gated optical imaging system with 0.4° gated FOV and 61 imaging channels is designed. In addition, a simplified prototype is developed, and a preliminary experiment of FOV gated imaging is performed near the ground. The results verify the capability of multiple stellar detections during daytime. The proposed optical imaging system has a strong capability of suppressing atmospheric background radiation and provides sufficient FOV gated imaging channels to enhance the probability of detecting multiple stars. It provides an effective technical way to develop all-time star sensors based on star pattern recognition and enables a completely autonomous attitude determination possible for platforms inside the atmosphere during daytime.

Paclitaxel Suppresses Hepatocellular Carcinoma Tumorigenesis Through Regulating Circ-BIRC6/miR-877-5p/YWHAZ Axis.

BACKGROUND: Paclitaxel is an effective chemotherapeutic agent for the treatment of cancer patients. Accumulating evidence suggests that circular RNAs (circRNAs) play critical roles in the occurrence and development of human cancers. However, there are few studies on interactions between paclitaxel and circRNAs in hepatocellular carcinoma (HCC). MATERIALS AND METHODS: Cell counting kit-8 (CCK-8) assay and colony formation assay were conducted to determine cell proliferation. Cell apoptosis was assessed by flow cytometry. The expression levels of circRNA baculoviral IAP repeat-containing 6 (circ-BIRC6), microRNA-877-5p (miR-877-5p), and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (YWHAZ) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The mice xenograft model was established to investigate the roles of circ-BIRC6 and paclitaxel in vivo. The interaction between miR-877-5p and circ-BIRC6 or YWHAZ was predicted by bioinformatics analysis and verified by dual-luciferase reporter assay. Western blot assay was applied for measuring the protein expression of YWHAZ. RESULTS: Paclitaxel suppressed HCC tumorigenesis through decreasing cell proliferation and accelerating apoptosis. Circ-BIRC6 and YWHAZ were upregulated, and miR-877-5p was downregulated in HCC tissues and cells. Paclitaxel treatment inhibited the expression of circ-BIRC6 and YWHAZ while promoted the expression of miR-877-5p. Circ-BIRC6 overexpression or miR-877-5p interference reversed the inhibitory effect of paclitaxel on HCC tumorigenesis. Moreover, miR-877-5p could specially bind to YWHAZ, and its knockdown abated the suppressive effect of circ-BIRC6 depletion on HCC tumorigenesis. Additionally, YWHAZ was identified as a direct target of miR-877-5p. Besides, circ-BIRC6 functioned as a molecular sponge of miR-877-5p to regulate YWHAZ expression. CONCLUSION: Paclitaxel limited HCC tumorigenesis via modulating circ-BIRC6/miR-877-5p/YWHAZ axis, providing a novel therapeutic approach for the treatment of HCC.

Transpiration cooling with bio-inspired structured surfaces.

Transpiration cooling is considered to be one of the most effective cooling methods for protecting components from ablation in extremely high temperature environments, so improving transpiration cooling efficiency is quite useful in practical applications. Living creatures always have the optimal properties for cooling after long-term evolution. This study proposes a novel transpiration cooling concept using a biomimetic non-smooth surface inspired by the earthworm's rough skin. The transpiration cooling efficiencies of porous plates with three different bio-inspired non-smooth surfaces - isosceles-trapezoid, right-angled-trapezoid and parallelogram grooves -are numerically investigated. The numerical model is validated by experimental data. The structure of the non-smooth surface dramatically affects the film thickness and surface heat convection intensity of transpiration cooling. The cooling efficiency is significantly improved by the parallelogram style non-smooth surface. The bio-inspired non-smooth surface successfully thickens the protective film and achieves a significantly better cooling performance. The protective film of transpiration cooling is thickened 22.7% while the transpiration cooling efficiency is significantly increased by 12% with the assistance of the bio-inspired non-smooth surface.

Experimental investigation of biomimetic self-pumping and self-adaptive transpiration cooling.

Transpiration cooling is an effective way to protect high heat flux walls. However, the pumps for the transpiration cooling system make the system more complex and increase the load, which is a huge challenge for practical applications. A biomimetic self-pumping transpiration cooling system was developed inspired by the process of trees transpiration that has no pumps. An experimental investigation showed that the water coolant automatically flowed from the water tank to the hot surface with a height difference of 80 mm without any pumps. A self-adaptive transpiration cooling system was then developed based on this mechanism. The system effectively cooled the hot surface with the surface temperature kept to about 373 K when the heating flame temperature was 1639 K and the heat flux was about 0.42 MW m-2. The cooling efficiency reached 94.5%. The coolant mass flow rate adaptively increased with increasing flame heat flux from 0.24 MW m-2 to 0.42 MW m-2 while the cooled surface temperature stayed around 373 K. Schlieren pictures showed a protective steam layer on the hot surface which blocked the flame heat flux to the hot surface. The protective steam layer thickness also increased with increasing heat flux.