[Morphogenetic changes in ambiol-treated regenerates of parent and transgenic potato plants]. / Morfogeneticheskie izmeneniia u regenerantov iskhodnykh i transgennykh rastenii kartofelia pod vliianiem ambiola.

The effects of ambiol, a new growth regulator, on the formation of leaves and roots in parent and defensin gene-transformed regenerants of potato Solanum tuberosum L. (cultivar Desire). Various concentrations of ambiol induced differences in morphogenetic parameters between parent and transgenic plants. In some cases, ambiol caused the formation of shoots without leaves or with rudimentary leaves. The data suggest that features of root and leaves formation in parent and transgenic regenerants induced by ambiol are due to changes in hormone balance in transgenic plants caused by expression of the defensin gene and the effect of ambiol on the plant hormonal balance.

[Effect of ambiol on stem growth in regenerants of source and transgenic potato plants]. / Vliianie ambiola na rost steblei u regenerantov iskhodnykh i transgennykh rastenii kartofelia.

The effects of ambiol, a new growth regulator, on stem growth and morphological features of stem development have been compared in regenerants of potato (Solanum tuberosum L., var. Desire) plants transgenic for a defensin gene and in original potato plants. The original and transgenic plants exhibited differences in shoot development, which were observed both in control settings (no ambiol) and in the presence of various ambiol concentrations. In addition to normal plants of both forms, plant regenerants with morphological deviations were present in ambiol-treated groups. It is suggested that the abnormal shoot development observed in original and transgenic potato plants treated with ambiol is associated with (a) hormonal changes caused by expression of the defensin gene in the transgenic plants and (b) effects of ambiol on the hormonal balance of the plants.

[Isolation of genetically modified potato plant containing the gene of defensive peptide from Amaranthus]. / Poluchenie geneticheski modifitsirovannykh rastenii kartofelia, nesushchikh gen zashchitnogo peptida amaranta.

The plants of potato (Solanum tuberosum L., var. Desire) have been transformed with a pH22Kneo vector carrying the gene ac2, encoding the fungicidal peptide (defensin) from the seed of amaranth (Amaranthus caudatus L.). The transformation involved co-cultivation of potato stem explants (excised from aseptically grown plants) and Agrobacterium tumefaciens on solid MS medium. Factors affecting in vitro regeneration of the explants and the transformation efficiency were optimized. Regenerated potato plants harboring the amaranth defensin gene were selected by two traits, growth and ability to form roots on kanamycin-supplemented MS medium. The transgenic state was confirmed PCR analysis of ac2 in tissues of the kanamycin-resistant plants. The transgenic organisms thus obtained differed from the original ambiol-treated plants in growth patterns and proton translocation across the plasma membrane of the tuber cells.