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1.
Atherosclerosis ; 228(2): 460-5, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23561648

ABSTRACT

OBJECTIVE: To measure the carotid intima-media-thickness (cIMT) and to assess its determinant factors in healthy adolescents. METHODS: 319 adolescents aged 12.5-17.5 years (135 boys, 184 girls) were enrolled in this cross-sectional study. cIMT and carotid diameter were measured by high-resolution B-mode ultrasound. Anthropometric parameters, blood pressure, physical activity (PA), aerobic fitness and dietary intakes were assessed. Socioeconomic status was determined with the family affluence scale (FAS 4). Serum biological markers (lipids, glucose, high-sensitivity C-reactive protein, soluble adhesion molecules) were measured in a subsample of 96 adolescents. RESULTS: Multiple regression analysis showed that cIMT was positively associated with truncal fat (p = 0.021) and negatively with FAS 4 (p = 0.002) independently of age and blood pressure. There were no significant associations between cIMT and PA, fitness and dietary intakes. In the subsample soluble intercellular adhesion molecule 1 was positively correlated with cIMT (p = 0.017), independently of truncal fat, age and blood pressure. CONCLUSIONS: Low socioeconomic conditions and increased truncal fat are associated with greater carotid intima-media-thickness in adolescents.


Subject(s)
Adiposity , Carotid Artery Diseases/etiology , Carotid Intima-Media Thickness , Intercellular Adhesion Molecule-1/blood , Obesity/complications , Socioeconomic Factors , Subcutaneous Fat/physiopathology , Adolescent , Age Factors , Biomarkers/blood , Carotid Artery Diseases/blood , Carotid Artery Diseases/diagnostic imaging , Carotid Artery Diseases/physiopathology , Chi-Square Distribution , Cross-Sectional Studies , Europe , Female , Humans , Life Style , Linear Models , Male , Multivariate Analysis , Obesity/blood , Obesity/physiopathology , Predictive Value of Tests , Risk Assessment , Risk Factors , Skinfold Thickness , Waist Circumference , Waist-Hip Ratio
2.
BMC Med Ethics ; 14: 7, 2013 Feb 15.
Article in English | MEDLINE | ID: mdl-23414421

ABSTRACT

BACKGROUND: To identify motivational factors linked to child health status that affected the likelihood of parents' allowing their child to participate in pediatric research. METHODS: Parents were invited to return their completed questionnaires anonymously to assess motivational factors and factors that might improve participation in pediatric research. RESULTS: Of 573 eligible parents, 261 returned the completed questionnaires. Of these, 126 were parents of healthy children (group 1), whereas 135 were parents of sick children who were divided into two groups according to the severity of their pathology, i.e., 99 ambulatory children (group 2) and 36 nonambulatory children (group 3). The main factor motivating participation in a pediatric clinical research study was "direct benefits for their child" (87.7%, 100%, and 100% for groups 1, 2, and 3, respectively). The other factors differed significantly between the three groups, depending on the child's health status (all p < 0.05). Factors that might have a positive impact on parental consent to the participation of their child in a pediatric clinical research study differed significantly (χ2 test, all p ≤ 0.04), depending on the child's health status. The main factor was "a better understanding of the study and its regulation" for the healthy children and ambulatory sick children groups (31.2% and 82.1%, respectively), whereas this was the third factor for the nonambulatory sick children group (50%). CONCLUSIONS: Innovative strategies should be developed based on a child's health status to improve information provision when seeking a child's participation in pediatric research. Parents would like to spend more time in discussions with investigators.


Subject(s)
Biomedical Research , Decision Making , Health Status , Parental Consent , Research Subjects , Adolescent , Adult , Child , Child, Preschool , Choice Behavior , Comprehension , Female , France , Humans , Male , Motivation , Severity of Illness Index , Surveys and Questionnaires
3.
PLoS Negl Trop Dis ; 6(7): e1704, 2012.
Article in English | MEDLINE | ID: mdl-22802974

ABSTRACT

BACKGROUND: Treatment of urinary schistosomiasis by chemotherapy remains challenging due to rapid re-infection and possibly to limited susceptibility to praziquantel treatment. Therefore, therapeutic vaccines represent an attractive alternative control strategy. The objectives of this study were to assess the safety and tolerability profile of the recombinant 28 kDa glutathione S-transferase of Schistosoma haematobium (rSh28GST) in healthy volunteers, and to determine its immunogenicity. METHODOLOGY: Volunteers randomly received 100 µg rSh28GST together with aluminium hydroxide (Alum) as adjuvant (n = 8), or Alum alone as a comparator (n = 8), twice with a 28-day interval between doses. A third dose of rSh28GST or Alum alone was administered to this group at day 150. In view of the results obtained, another group of healthy volunteers (n = 8) received two doses of 300 µg of rSh28GST, again with a 28-day interval. A six-month follow-up was performed with both clinical and biological evaluations. Immunogenicity of the vaccine candidate was evaluated in terms of specific antibody production, the capacity of sera to inhibit enzymatic activity of the antigen, and in vitro cytokine production. PRINCIPAL FINDINGS: Among the 24 healthy male participants no serious adverse events were reported in the days or weeks after administration. Four subjects under rSh28GST reported mild reactions at the injection site while a clinically insignificant increase in bilirubin was observed in 8/24 subjects. No hematological nor biochemical evidence of toxicity was detected. Immunological analysis showed that rSh28GST was immunogenic. The induced Th2-type response was characterized by antibodies capable of inhibiting the enzymatic activity of rSh28GST. CONCLUSIONS: rSh28GST in Alum did not induce any significant toxicity in healthy adults and generated a Th2-type immune response. Together with previous preclinical results, the data of safety, tolerability and quality of the specific immune response provide evidence that clinical trials with rSh28GST could be continued in humans as a potential vaccine candidate against urinary schistosomiasis.


Subject(s)
Antigens, Helminth/immunology , Glutathione Transferase/immunology , Helminth Proteins/immunology , Schistosoma haematobium/immunology , Schistosomiasis haematobia/therapy , Vaccination/adverse effects , Vaccination/methods , Adjuvants, Immunologic/administration & dosage , Adolescent , Adult , Alum Compounds/administration & dosage , Animals , Antibodies, Helminth/blood , Antigens, Helminth/administration & dosage , Antigens, Helminth/genetics , Cytokines/metabolism , Drug-Related Side Effects and Adverse Reactions/epidemiology , Glutathione Transferase/administration & dosage , Glutathione Transferase/genetics , Healthy Volunteers , Helminth Proteins/administration & dosage , Helminth Proteins/genetics , Humans , Male , Neutralization Tests , Recombinant Proteins/administration & dosage , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Schistosoma haematobium/genetics , Vaccines, Synthetic/administration & dosage , Vaccines, Synthetic/genetics , Vaccines, Synthetic/immunology , Young Adult
4.
J Psychiatr Res ; 46(6): 790-6, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22425487

ABSTRACT

BACKGROUND: Road Traffic Accidents (RTA) are most probably the leading cause of post traumatic stress disorder (PTSD) in developed countries. The autonomic nervous system (ANS) disturbances, due to psychological trauma, are part of the pathophysiology of PTSD. The aim of the present study was to determine whether early heart rate variability (HRV) measurement, a biomarker of the ANS function, could act as a predictor of PTSD development after a RTA. METHODS: We prospectively investigated 35 survivors of RTA with both physical injury and psychological trauma. HRV data were obtained from 24-h Holter ECG monitoring, which was performed on the second day after the accident. Time domain analysis was applied to the inter-beat (RR) interval time series to calculate the various parameters of HRV. PTSD status was assessed 2 and 6 months after RTA. RESULTS: There was a global diminution of HRV measurements in the PTSD group at both 2 and 6 months. The variability index was the best predictor of PTSD with the area under the receiveroperating curve for discriminating PTSD at 6 months at 0.92 (95% CI: 0.785; 1.046). A cut-off at 2.19% yielded a sensitivity of 85.7% and a specificity of 81.8% for PTSD. Positive and negative predictive values were respectively 75% and 90%. However, initial heart rate (HR) data were relevant at 2 months but not at 6 months. CONCLUSION: RTA survivors exhibiting lower parasympathetic modulation of HR, indexed by temporal analysis of HRV, are more susceptible to developing PTSD as a short and long-term outcome.


Subject(s)
Accidents, Traffic , Heart Rate/physiology , Stress Disorders, Post-Traumatic , Survivors/psychology , Adolescent , Adult , Aged , Electrocardiography , Female , Follow-Up Studies , Humans , Male , Middle Aged , Predictive Value of Tests , Psychiatric Status Rating Scales , ROC Curve , Stress Disorders, Post-Traumatic/diagnosis , Stress Disorders, Post-Traumatic/etiology , Stress Disorders, Post-Traumatic/psychology , Time Factors , Young Adult
5.
Trials ; 13: 27, 2012 Mar 27.
Article in English | MEDLINE | ID: mdl-22452964

ABSTRACT

BACKGROUND: In order to facilitate multinational clinical research, regulatory requirements need to become international and harmonised. The EU introduced the Directive 2001/20/EC in 2004, regulating investigational medicinal products in Europe. METHODS: We conducted a survey in order to identify the national regulatory requirements for major categories of clinical research in ten European Clinical Research Infrastructures Network (ECRIN) countries-Austria, Denmark, France, Germany, Hungary, Ireland, Italy, Spain, Sweden, and United Kingdom-covering approximately 70% of the EU population. Here we describe the results for regulatory requirements for typical investigational medicinal products, in the ten countries. RESULTS: Our results show that the ten countries have fairly harmonised definitions of typical investigational medicinal products. Clinical trials assessing typical investigational medicinal products require authorisation from a national competent authority in each of the countries surveyed. The opinion of the competent authorities is communicated to the trial sponsor within the same timelines, i.e., no more than 60 days, in all ten countries. The authority to which the application has to be sent to in the different countries is not fully harmonised. CONCLUSION: The Directive 2001/20/EC defined the term 'investigational medicinal product' and all regulatory requirements described therein are applicable to investigational medicinal products. Our survey showed, however, that those requirements had been adopted in ten European countries, not for investigational medicinal products overall, but rather a narrower category which we term 'typical' investigational medicinal products. The result is partial EU harmonisation of requirements and a relatively navigable landscape for the sponsor regarding typical investigational medicinal products.


Subject(s)
Biomedical Research/legislation & jurisprudence , Device Approval/legislation & jurisprudence , Drug Approval/legislation & jurisprudence , Drugs, Investigational/therapeutic use , Government Regulation , Health Policy , Biomedical Research/standards , Consumer Product Safety/legislation & jurisprudence , Device Approval/standards , Drugs, Investigational/adverse effects , Europe , Guideline Adherence , Guidelines as Topic , Humans , International Cooperation/legislation & jurisprudence , Surveys and Questionnaires
6.
Stroke Res Treat ; 2012: 614925, 2012.
Article in English | MEDLINE | ID: mdl-21912754

ABSTRACT

In the present subanalysis of a cross-sectional study showing the favorable effect of prior transient ischemia, leisure-time physical activity, and lipid-lowering drug therapy on stroke severity, we aimed to evaluate whether previous physical activity was dose dependently associated to minor stroke (NIHSS 0-3) and to identify possible underlying factors. Among 362 consecutive patients, less severe stroke was related to weekly exercise duration prior to stroke (no exercise: 36.1%; <2 hours: 49.3%; 2-5 hours: 58.8%; >5 hours: 64.0%; P = 0.003). Only weak and moderate exercise practices were protective (weak: 50.0%; moderate: 79.3%; heavy: 22.2%; P < 0.0001). Such a beneficial effect was observed independently of age and was associated with a trend to a lower frequency of arterial hypertension, alcohol abuse, and a better metabolic profile. Besides other therapeutic approaches, physical activity may be a simple way to decrease cerebral ischemia severity.

7.
PLoS One ; 7(12): e52223, 2012.
Article in English | MEDLINE | ID: mdl-23300620

ABSTRACT

BACKGROUND & AIMS: Recent studies reported a role for more than 70 genes or loci in the susceptibility to Crohn's disease (CD). However, the impact of these associations in clinical practice remains to be defined. The aim of the study was to analyse the relationship between genotypes and phenotypes for the main 53 CD-associated polymorphisms. METHOD: A cohort of 798 CD patients with a median follow up of 7 years was recruited by tertiary adult and paediatric gastroenterological centres. A detailed phenotypic description of the disease was recorded, including clinical presentation, response to treatments and complications. The participants were genotyped for 53 CD-associated variants previously reported in the literature and correlations with clinical sub-phenotypes were searched for. A replication cohort consisting of 722 CD patients was used to further explore the putative associations. RESULTS: The NOD2 rare variants were associated with an earlier age at diagnosis (p = 0.0001) and an ileal involvement (OR = 2.25[1.49-3.41] and 2.77 [1.71-4.50] for rs2066844 and rs2066847, respectively). Colonic lesions were positively associated with the risk alleles of IL23R rs11209026 (OR = 2.25 [1.13-4.51]) and 6q21 rs7746082 (OR = 1.60 [1.10-2.34] and negatively associated with the risk alleles of IRGM rs13361189 (OR = 0.29 [0.11-0.74]) and DEFB1 rs11362 (OR = 0.50 [0.30-0.80]). The ATG16L1 and IRGM variants were associated with a non-inflammatory behaviour (OR = 1.75 [1.22-2.53] and OR = 1.50 [1.04-2.16] respectively). However, these associations lost significance after multiple testing corrections. The protective effect of the IRGM risk allele on colonic lesions was the only association replicated in the second cohort (p = 0.03). CONCLUSIONS: It is not recommended to genotype the studied polymorphisms in routine practice.


Subject(s)
Crohn Disease/genetics , Genotyping Techniques , Phenotype , Polymorphism, Single Nucleotide , Adolescent , Adult , Age of Onset , Case-Control Studies , Cohort Studies , Crohn Disease/complications , Crohn Disease/epidemiology , Crohn Disease/therapy , Female , Humans , Male , Medical Records , Sex Factors , Smoking/adverse effects , Young Adult
9.
BMC Psychiatry ; 11: 1, 2011 Jan 02.
Article in English | MEDLINE | ID: mdl-21194496

ABSTRACT

BACKGROUND: Suicide attempts (SA) constitute a serious clinical problem. People who attempt suicide are at high risk of further repetition. However, no interventions have been shown to be effective in reducing repetition in this group of patients. METHODS/DESIGN: Multicentre randomized controlled trial. We examine the effectiveness of "ALGOS algorithm": an intervention based in a decisional tree of contact type which aims at reducing the incidence of repeated suicide attempt during 6 months. This algorithm of case management comprises the two strategies of intervention that showed a significant reduction in the number of SA repeaters: systematic telephone contact (ineffective in first-attempters) and "Crisis card" (effective only in first-attempters). Participants who are lost from contact and those refusing healthcare, can then benefit from "short letters" or "postcards". DISCUSSION: ALGOS algorithm is easily reproducible and inexpensive intervention that will supply the guidelines for assessment and management of a population sometimes in difficulties with healthcare compliance. Furthermore, it will target some of these subgroups of patients by providing specific interventions for optimizing the benefits of case management strategy.


Subject(s)
Case Management/standards , Mental Disorders/therapy , Suicide Prevention , Suicide, Attempted/psychology , Adult , Algorithms , Case Management/organization & administration , Crisis Intervention/standards , Decision Trees , Humans , Incidence , Mental Disorders/psychology , Research Design , Risk Assessment , Secondary Prevention , Suicide/psychology , Suicide/statistics & numerical data , Suicide, Attempted/prevention & control , Suicide, Attempted/statistics & numerical data , Treatment Outcome
10.
Eur J Pediatr ; 170(6): 719-29, 2011 Jun.
Article in English | MEDLINE | ID: mdl-20978785

ABSTRACT

UNLABELLED: Increased carotid intima-media thickness (cIMT) in adults may be caused by a childhood exposure to cardiovascular risk factors. We systematically reviewed observational studies to determine whether obesity, insulin-dependent diabetes mellitus (IDDM), dyslipidemia (DL), hypertension (HT), and chronic renal failure (CRF) are associated with increased cIMT in children and adolescent patients compared with control subjects. We performed a PubMed literature search from January 1986 to February 2010. Two reviewers separately verified the inclusion criteria of relevant studies for the objective of the review. The data extracted in the patient and control groups were sample size, age, gender, cIMT measurement methods, cIMT values, and statistical analysis results. From 348 citations, 65 cross-sectional studies (2 cited twice) with case-control design met the inclusion criteria: 26 in obesity, 14 in IDDM, 11 in DL, 8 in HT, and 8 in CRF. cIMT measurement protocols varied according to the studies, with measurements being performed on the common carotid artery in 65/67 cases and on the far wall in 57/67 cases. From the 67 studies cited, 22/26 reported a significantly increased cIMT in obese children and adolescents compared with the control group, 8/14 in IDDM patients, 10/11 in DL patients, 7/8 in HT patients, and 8/8 in CRF patients. CONCLUSION: Despite the heterogeneity of ultrasound measurement methods, cIMT was significantly increased in 55 out of the 67 cited studies, confirming early vascular damages in pediatric populations with an increased future risk for vascular diseases.


Subject(s)
Cardiovascular Diseases/etiology , Carotid Arteries/diagnostic imaging , Tunica Intima/diagnostic imaging , Tunica Media/diagnostic imaging , Adolescent , Adult , Aging/pathology , Carotid Arteries/pathology , Child , Diabetes Mellitus, Type 1/complications , Dyslipidemias/complications , Humans , Hypertension/complications , Kidney Failure, Chronic/complications , Obesity/complications , Observation , Risk Factors , Tunica Intima/pathology , Tunica Media/pathology , Ultrasonography
11.
Trials ; 11: 104, 2010 Nov 12.
Article in English | MEDLINE | ID: mdl-21073691

ABSTRACT

BACKGROUND: 'Compassionate use' programmes allow medicinal products that are not authorised, but are in the development process, to be made available to patients with a severe disease who have no other satisfactory treatment available to them. We sought to understand how such programmes are regulated in ten European Union countries. METHODS: The European Clinical Research Infrastructures Network (ECRIN) conducted a comprehensive survey on clinical research regulatory requirements, including questions on regulations of 'compassionate use' programmes. Ten European countries, covering approximately 70% of the EU population, were included in the survey (Austria, Denmark, France, Germany, Hungary, Ireland, Italy, Spain, Sweden, and the UK). RESULTS: European Regulation 726/2004/EC is clear on the intentions of 'compassionate use' programmes and aimed to harmonise them in the European Union. The survey reveals that different countries have adopted different requirements and that 'compassionate use' is not interpreted in the same way across Europe. Four of the ten countries surveyed have no formal regulatory system for the programmes. We discuss the need for 'compassionate use' programmes and their regulation where protection of patients is paramount. CONCLUSIONS: 'Compassionate use' is a misleading term and should be replaced with 'expanded access'. There is a need for expanded access programmes in order to serve the interests of seriously ill patients who have no other treatment options. To protect these patients, European legislation needs to be more explicit and informative with regard to the regulatory requirements, restrictions, and responsibilities in expanded access programmes.


Subject(s)
Biomedical Research , Compassionate Use Trials , Clinical Trials as Topic , Compassionate Use Trials/legislation & jurisprudence , Europe , Humans
12.
Therapie ; 65(3): 149-50, 2010.
Article in French | MEDLINE | ID: mdl-27392979
13.
Fundam Clin Pharmacol ; 24(2): 239-45, 2010 Apr.
Article in English | MEDLINE | ID: mdl-19719551

ABSTRACT

Oral hormone therapy is associated with an increased risk of venous thrombosis. Drug agencies recommend the use of the lowest efficient dose to treat menopausal symptoms for a better risk/ratio profile, although this profile has not been totally investigated yet. The aim of the study was to compare the effect of the standard dose of 17beta-estradiol to a lower one on thrombin generation (TG). In a 2-month study, healthy menopausal women were randomized to receive daily 1mg or 2 mg of 17beta-estradiol (E1, n = 24 and E2, n = 26; respectively) with 10 mg dydrogesterone or placebo (PL, n = 22). Plasma levels factors VII, X, VIII and II were assessed before and after treatment as well as Tissue factor triggered TG, which allows the investigation of the different phases of coagulation process. The peak of thrombin was higher in hormone therapy groups (E1: 42.39 +/- 50.23 nm, E2: 31.08 +/- 85.86 nm vs. 10.52 +/- 40.63 nm in PL, P = 0.002 and P = 0.01). Time to reach the peak was also shortened (PL: 0.26 +/- 0.69 min vs. E1: -0.26 +/- 0.80 min, E2: -0.55 +/- 0.79 min, P <10(-3) for both comparisons) and mean rate index of the propagation phase of TG was significantly increased. Among the studied clotting factors, only the levels of FVII were significantly increased after treatment administration. The two doses of 17beta-estradiol induced in a similar degree an acceleration of the initiation and propagation phase of tissue factor triggered thrombin generation and a significant increase of FVII coagulant activity.


Subject(s)
Dydrogesterone/pharmacology , Estradiol/pharmacology , Menopause , Thrombin/drug effects , Administration, Oral , Aged , Blood Coagulation Factors/drug effects , Blood Coagulation Factors/metabolism , Dose-Response Relationship, Drug , Double-Blind Method , Drug Therapy, Combination , Dydrogesterone/administration & dosage , Estradiol/administration & dosage , Estrogens/administration & dosage , Estrogens/pharmacology , Female , Humans , Middle Aged , Progestins/administration & dosage , Progestins/pharmacology , Thrombin/metabolism , Time Factors
14.
Trials ; 10: 95, 2009 Oct 16.
Article in English | MEDLINE | ID: mdl-19835581

ABSTRACT

BACKGROUND: Thorough knowledge of the regulatory requirements is a challenging prerequisite for conducting multinational clinical studies in Europe given their complexity and heterogeneity in regulation and perception across the EU member states. METHODS: In order to summarise the current situation in relation to the wide spectrum of clinical research, the European Clinical Research Infrastructures Network (ECRIN) developed a multinational survey in ten European countries. However a lack of common classification framework for major categories of clinical research was identified, and therefore reaching an agreement on a common classification was the initial step in the development of the survey. RESULTS: The ECRIN transnational working group on regulation, composed of experts in the field of clinical research from ten European countries, defined seven major categories of clinical research that seem relevant from both the regulatory and the scientific points of view, and correspond to congruent definitions in all countries: clinical trials on medicinal products; clinical trials on medical devices; other therapeutic trials (including surgery trials, transplantation trials, transfusion trials, trials with cell therapy, etc.); diagnostic studies; clinical research on nutrition; other interventional clinical research (including trials in complementary and alternative medicine, trials with collection of blood or tissue samples, physiology studies, etc.); and epidemiology studies. Our classification was essential to develop a survey focused on protocol submission to ethics committees and competent authorities, procedures for amendments, requirements for sponsor and insurance, and adverse event reporting following five main phases: drafting, consensus, data collection, validation, and finalising. CONCLUSION: The list of clinical research categories as used for the survey could serve as a contribution to the, much needed, task of harmonisation and simplification of the regulatory requirements for clinical research in Europe.


Subject(s)
Biomedical Research , Biomedical Research/legislation & jurisprudence , Clinical Trials as Topic , Data Collection , Europe , Humans
15.
Am J Gastroenterol ; 104(7): 1745-53, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19471251

ABSTRACT

OBJECTIVES: Anti-Saccharomyces cerevisiae antibodies (ASCAs) are present in 50-60% of patients with Crohn's disease (CD) and in 20-25% of their healthy relatives (HRs). The yeast, Candida albicans, has been shown to generate ASCAs, but the presence of C. albicans in the digestive tract of CD patients and their HRs has never been investigated. Therefore, we studied C. albicans carriage in familial CD and its correlation with ASCAs. METHODS: Study groups consisted of 41 CD families composed of 129 patients and 113 HRs, and 14 control families composed of 76 individuals. Mouth swabs and stool specimens were collected for isolation, identification, and quantification of yeasts. Serum samples were collected for detection of ASCAs and anti-C. albicans mannan antibodies (ACMAs). RESULTS: C. albicans was isolated significantly more frequently from stool samples from CD patients (44%) and their HRs (38%) than from controls (22%) (P<0.05). The prevalence of ACMAs was similar between CD patients, their HRs, and controls (22, 19, and 21%, respectively, P=0.845), whereas the prevalence of ASCAs was significantly increased in CD families (72 and 34% in CD and HRs, respectively, in contrast to 4% in controls, P<0.0001). AMCA levels correlated with C. albicans colonization in all populations. ASCA levels correlated with C. albicans colonization in HRs but not in CD patients. CONCLUSIONS: CD patients and their first-degree HRs are more frequently and more heavily colonized by C. albicans than are controls. ASCAs correlate with C. albicans colonization in HRs but not in CD. In HRs, ASCAs could result from an altered immune response to C. albicans. In CD, a subsequent alteration in sensing C. albicans colonization could occur with disease onset.


Subject(s)
Candida albicans/genetics , Candidiasis/genetics , Crohn Disease/genetics , Crohn Disease/microbiology , Saccharomyces cerevisiae/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Analysis of Variance , Antibodies, Fungal/analysis , Candida albicans/immunology , Candidiasis/immunology , Case-Control Studies , Colony Count, Microbial , Enzyme-Linked Immunosorbent Assay , Female , France , Humans , Incidence , Logistic Models , Male , Middle Aged , Pedigree , Probability , Reference Values , Risk Assessment , Saccharomyces cerevisiae/immunology , Sensitivity and Specificity , Severity of Illness Index , Yeasts/genetics , Yeasts/immunology , Young Adult
16.
Eur J Cancer ; 45(10): 1871-6, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19361981

ABSTRACT

Some host-related factors may predict the risk of metastasis after surgery of colorectal cancer (CRC). The endothelial adhesion molecule E-selectin is implicated in the metastatic spread of CRC. We postulated that some polymorphisms within the E-selectin gene, especially the S128R polymorphism, may increase the risk of metastases by facilitating adhesion of tumour cells to the endothelium. We collected blood samples for DNA extraction from 264 patients treated for stage II or III CRC and from 310 healthy controls in order to assess three polymorphisms within the E-selectin gene (S128R, G98T and L554F) and one within the P-selectin gene (V640L). Genotypes were analysed by the allelic discrimination TaqMan real-time PCR assay. The S128R polymorphism was detected in 59 patients (22.3%) and was strictly correlated with the G98T polymorphism. In multivariate analysis, the S128R polymorphism was associated with shorter event-free survival (EFS) and overall survival (OS) in the whole population (EFS: P=.003, HR 1.82, 95% CI 1.23-2.70; OS: P<10(-4), HR 4.31, 95% CI 2.46-10.99), in patients with stage II CRC(EFS: P=.04, HR 1.92, 95% CI 1.02-3.60; OS: P=.02, HR 4.44, 95% CI 1.16-17.03), and in patients with stage III CRC (EFS: P=.04, HR 1.68, 95% CI 1.01-2.80; OS: P=.001, HR 4.04, 95% CI 1.73-9.46). L554F and V640L polymorphisms had no prognostic value. The S128R polymorphism is a constitutional factor associated with a higher risk of relapse and death in patients treated for CRC. This polymorphism detection may permit better selection of patients suitable for adjuvant therapy, especially among those with stage II disease.


Subject(s)
Adenocarcinoma/genetics , Colorectal Neoplasms/genetics , E-Selectin/genetics , Adenocarcinoma/pathology , Adenocarcinoma/therapy , Adult , Aged , Aged, 80 and over , Colorectal Neoplasms/pathology , Colorectal Neoplasms/therapy , DNA, Neoplasm/genetics , Female , Follow-Up Studies , Genotype , Humans , Male , Middle Aged , Neoplasm Staging , P-Selectin/genetics , Polymorphism, Genetic , Prognosis , Survival Analysis , Treatment Outcome
17.
Therapie ; 62(3): 259-70, 2007.
Article in English | MEDLINE | ID: mdl-17803895

ABSTRACT

The key to health promotion and disease prevention in the 21st century is to establish an environment that supports positive health behaviour and healthy lifestyle from childhood. The HELENA project includes cross-sectional, crossover and pilot community intervention multi-centre studies, as an integrated approach to the above-mentioned problem. Dietary intake, nutrition knowledge and eating attitudes, food choices and preferences, body composition, biochemical, physical activity and fitness and genotype (to analyse gene-nutrient and gene-environment interactions) assessment will provide the full information about the nutritional and lifestyle status of the European adolescents. The requirements for health promoting foods will be also identified, and three sensory acceptable products for adolescents will be developed. Harmonization and standardisation of the assessments for both scientific and technological objectives should result in reliable and comparable data of a representative sample of European adolescents. This will contribute to understand why health-related messages are not being as effective as expected in the adolescent population. A realistic intervention strategy will be proposed in order to achieve the goals of understanding and effectively enhancing nutritional and lifestyle habits of adolescents in Europe.


Subject(s)
Adolescent Nutritional Physiological Phenomena , Health Promotion , Life Style , Adolescent , Diet , European Union , Food Preferences , Genotype , Humans , Motor Activity , Physical Fitness
18.
Therapie ; 62(1): 45-8, 2007.
Article in English | MEDLINE | ID: mdl-17374347

ABSTRACT

AIMS: To establish the incidence of early adverse events surrounding direct current (DC) cardioversion of persistent atrial fibrillation in an unselected patient cohort and to describe and analyse these complications. METHODS: Prospective study over a three-month period (February, March and April 2000). Outcome measures included all serious adverse events during the hospitalisation for DC cardioversion. Six hundred and eighty-four DC cardioversion were performed on 659 patients. RESULTS: The rate of adverse events was 4.4% including 1.3% bradycardia, 0.3% ventricular arrhythmia, 0.3% QT increase, 1% serious haemorrhages, 0.4% death and 1.2% miscellaneous adverse events. CONCLUSION: The perceived tolerance to DC cardioversion in atrial fibrillation should be amended with the 4.4% serious early adverse events.


Subject(s)
Atrial Fibrillation/complications , Electric Countershock/adverse effects , Aged , Arrhythmias, Cardiac/epidemiology , Arrhythmias, Cardiac/etiology , Atrial Fibrillation/physiopathology , Atrial Fibrillation/therapy , Bradycardia/epidemiology , Bradycardia/etiology , Cohort Studies , Female , Hemorrhage/epidemiology , Hemorrhage/etiology , Humans , Long QT Syndrome/epidemiology , Long QT Syndrome/etiology , Male , Prospective Studies , Treatment Outcome
19.
Exp Clin Psychopharmacol ; 15(1): 115-22, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17295590

ABSTRACT

The effect of benzodiazepines on attention has been the object of few investigations. Studies using the spatial cueing paradigm (Posner's paradigm) have reported inconsistent results, which are likely due to methodological and/or dose differences but suggest impaired disengagement of attention from the cue to the target. The authors investigated the effect of a benzodiazepine (diazepam) on attentional shifting in the temporal domain. The attentional blink effect refers to difficulties in detecting a target if it follows the identification of a previous target occurring within a temporal window of 200-400 ms. The authors assessed whether the duration of the attentional blink was affected by diazepam. Streams of 15 real-world scenes displaying a road were presented for 50 ms each. A city name (target) appeared at Serial Positions 2, 3, or 4 of each stream. A vehicle (probe) appeared at different intervals following the city name. In a dual-task condition, participants were asked to report the city name and whether a vehicle was present. In a control condition, participants had to report only the presence of a vehicle and ignore the city name. Thirty-six healthy volunteers were assigned to 3 groups (placebo, diazepam 0.1 mg/kg, or 0.3 mg/kg). Diazepam increased both the magnitude and duration of the attentional blink effect. Participants treated with a high dose of diazepam needed more than 600 ms to detect a vehicle following identification of the name. Results suggest that diazepam at a therapeutic dosage affects attentional shifting in the temporal domain and impairs dual-task performance.


Subject(s)
Attention/drug effects , Diazepam/pharmacology , Visual Perception/drug effects , Administration, Oral , Adolescent , Adult , Analysis of Variance , Anti-Anxiety Agents/administration & dosage , Anti-Anxiety Agents/pharmacology , Automobile Driving , Blinking/drug effects , Diazepam/administration & dosage , Dose-Response Relationship, Drug , Double-Blind Method , Female , Humans , Male , Pattern Recognition, Visual/drug effects , Perceptual Masking , Photic Stimulation , Task Performance and Analysis , Time Factors
20.
BMJ ; 332(7552): 1241-5, 2006 May 27.
Article in English | MEDLINE | ID: mdl-16735333

ABSTRACT

OBJECTIVE: To determine the effects over one year of contacting patients by telephone one month or three months after being discharged from an emergency department for deliberate self poisoning compared with usual treatment. DESIGN: Multicentre, randomised controlled trial. SETTING: 13 emergency departments in the north of France. PARTICIPANTS: 605 people discharged from an emergency department after attempted suicide by deliberate self poisoning. INTERVENTION: The intervention consisted of contacting patients by telephone at one month or three months after discharge from an emergency department for attempted suicide to evaluate the success of recommended treatment or to adjust treatment. Control patients received treatment as usual, in most cases referral back to their general practitioner. MAIN OUTCOME MEASURES: The primary outcome measures were proportion of participants who reattempted suicide, number of deaths by suicide, and losses to follow-up at 13 months' follow-up. Secondary outcome measures were types and number of contacts with health care. RESULTS: On an intention to treat basis, the three groups did not differ significantly for further suicide attempts, deaths by suicide, or losses to follow-up: contact at one month (intervention 23% (34/147) v controls 30% (93/312), difference 7%, 95% confidence interval - 2% to 15%), three months (25% (36/146) v 30%, difference 5%, - 4% to 14%). Participants contacted at one month were less likely at follow-up to report having reattempted suicide (12% v 22% in control group, difference 10%, 2% to 18%). CONCLUSION: Contacting people by telephone one month after being discharged from an emergency department for deliberate self poisoning may help reduce the number of reattempted suicides over one year.


Subject(s)
Suicide, Attempted/prevention & control , Telephone , Adolescent , Adult , Aged , Drug Overdose/prevention & control , Emergency Service, Hospital/statistics & numerical data , Humans , Middle Aged , Patient Acceptance of Health Care/statistics & numerical data , Self-Injurious Behavior/prevention & control , Suicide, Attempted/statistics & numerical data
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