[Phages of halophilic vibrios]. / Fagi galofil'nykh vibrionov.

The morphology and antigenic properties of 34 phages of Vibrio parahaemolyticus and V. alginolyticus strains isolated from Black Sea water, as well as their range of lytic activity and specificity of action, have been studied. Most of these phages have been found to lyze both V. parahaemolyticus and V. alginolyticus strains. In addition, highly specific phages capable of lyzing only V. parahaemolyticus serotype O5:K15, have been detected. The latter fact is a precedent indicating the possibility of search for other typing phages and the development of the scheme for the phage typing of V. parahaemolyticus in future.

[The isolation of Vibrio fluvialis on the territory of the USSR]. / O vydelenii Vibrio fluvialis na territorii SSSR.

For the first time V. fluvialis strains were detected on the territory of the USSR. The taxonomic position of these vibrios was determined by their nucleotide DNA composition (the content of guanine + cytosine was 49.3-51.0 mole%) and the characteristic features of their phenotype. The individual features of the strains consisted in their capacity for agglutination with cholera antisera, groups 01 and Inaba, in diagnostic dilutions in the presence of differences in genomes and phenotypes with cholera vibrios. Molecular hybridization DNA-DNA also gave no confirmation of their relationship to cholera vibrios (23-26% homology). The comparative study of V. fluvialis strains from the USSR and other countries by a broader set of their phenotypical signs confirmed their identity.

[Clinico-epidemiological characteristics of diseases caused by halophilic vibrios along the coast of the Sea of Azov]. / Kliniko-épidemiologicheskaia kharakteristika zabolevanii, vyzvannykh galofil'nymi vibrionami na poberezh'e Azovskogo moria.

The epidemiological and clinical features of alimentary toxinfections caused by halophilic vibrios on the Crimean coast of the Sea of Azov at the period of 1976-1984 were studied. Toxinfections were linked mainly with the use of sea-food subjected to different kinds of culinary treatment and took the course of gastroenteritis. To prevent toxinfections caused by halophilic vibrios, the technology, as well as sanitary and hygienic norms, should be strictly observed in the production of sea-food; besides, special methods of bacteriological diagnosis should be introduced into practice at laboratories of medical institutions and fish-processing plants.

[Relationship between hemolysin and hemolysin-destructive factor activities in Vibrio cholerae of Eltor biovar]. / Vzaimootnoshenie aktivnostei gemolizina i gemolizindestruktivnogo faktora u Vibrio cholerae biovara Eltor.

The study of hemolysin activity, hemolysin-destructive factor (HDF) activity and cholerogenicity in 143 Vibrio eltor strains has revealed the existance of close relationship between cholerogenicity and hemolysin production, as well as between cholerogenicity and HDF activity. The negative character of conjugation between the HDF activity and the hemolytic activity of the strains under study has been established, which allows one to suggest the possibility of the regulating action of hemolysin on the synthesis of HDF (enterotoxin).

[Taxonomic position of representatives of the species Vibrio cholerae]. / O taksonomicheskom polozhenii nekotorykh predstavitelei vida Vibrio cholerae.

The data contained in the works of some foreign authors who have reconsidered the taxonomic position of V. metschnikovii are analyzed. In contrast to the views expressed by Gamaleya whi believed this microorganism to be the modified cholera vibrio, the foreign authors identify it with the "Kommabacillus der Cholera nostras" described by Finkler and Prior (1884) and classify it with saprophytes widely spread in the environment. These points of view were refuted by the data resulting from the study of the strains of V. metschnikovii and the Finkler - Prior vibrio from Soviet collections. The variant from the Odessa Institute of Epidemiology and Microbiology has been found to be most similar to the original characteristic given by Gamaleya and should be considered the lectotype of V. metschnikovii.

[Halophilic Black Sea vibrios and their role in human pathology]. / Galofil'nye vibriony Chernogo moria i ikh rol' v patologii cheloveka.

During the period of 4 years 558 halophil vibrio strains (258 V. parahaemolyticus strains and 300 V. angiolyticus strains) were isolated from the water and from the representatives of the hydrobios of the Black Sea. The vibrios were isolated the whole year round, but during the winter and spring months their lowest concentration in water was observed, while in September-October it reached its maximum. During these 4 years 102 cases of gastroenteric diseases were registered, and in some of the patients halophil vibrios were isolated. In most cases the diseases developed after the consumption of sea fish and other sea products, as well as salted vegetables. The diseases had mainly a sporadic character. 90% of the strains isolated from humans belonged to serotypes O4 : K12 and O4 : K8; besides, the presence of serotypes O3 : K33, O3 : K57, O5 : K47, O6 : K46 and O10 : K52 was revealed.

[Halophilic vibrios isolated from the Sea of Azov]. / Galofil'nye vibriony, vydelennye iz Azovskogo moria.

Halophilic vibrios (V. parahaemolyticus and V. alginolyticus) were for the first time isolated from the Azov sea in the Eisk district. These vibrios proved to be typical representatives of Vibrio genus and were distinctly differentiated by species according to the following signs: saccharose splitting, growth in peptone water with 10% NaCl solution, and acetylmethylcarbinol production. Swarming capacity on 1.5% agar with 3% NaCl proved to be a more variable sign. All the cultures isolated were typed by means of Japanese O- and K-sera and had the following antigenic formulae: O1:K32; O2:K28; O3:K57; O5:K17; O6:K18. Only in 3 cases it was impossible to determine completely the antigenic formula of vibrios referred by the O-antigen type +o the 7th and 11th groups. Thus, parahemolytic vibrios isolated from the Azov sea were referred to the serological types revealed in Japan in the sea water, hydrobionts and feces of patients with acute gastrointestinal diseases.

[Use of media with antibiotics for isolating resistant forms of the cholera vibrios]. / Primenenie sred c antibiotikami dlia vydeleniia rezistentnykh form kholernykh vibrionov

Higher stability of resistance to tetracycline in the polyresistant strain of V. eltor under conditions of macroorganism as compared to nutrient media was found experimentally. To increase isolation of the resistant forms of the cholera vibrio it was recommended to use agar with tetracycline or other antibiotics depending on the particular case in addition to the routine media.

[Isolation of parahemolytic vibrions from persons with acute gastrointestinal diseases]. / O vydelenii paragemoliticheskikh vibrionov ot liudei s ostrymi zheludochno-kishechnymi zabolevaniiami

Galophilic vibrios were for the first time in the Soviet Union isolated from the patients with gastrointestinal diseases in Novorossiisk during the summer-autumn period of 1973. The greatest number of strains of the parahemolytic vibrios was isolated in August and September, during intensive fishing season, from the patients in whom the disease usually developed 6 to 12 hours after eating the sea fish. The parahemolytic vibrios isolated from the patients were typical by all signs and produced a marked hemolysis on the Wagatsuma medium. The halophilic vibrios are inhabitants of the Black Sea. During the years of 1972-1973 there were isolated 109 strains of V. paraheamolyticus and 133 strains of V. alginolyticus from the sea water and various hydrobionts.