ABSTRACT
Summary BoxO_ST_ABSWhat is already known about this subject?C_ST_ABSThe Wuhan city in China had a much higher mortality rate (Feb 10th statistics: 748 death/18,454 diagnosis =4.05%; Apr 24th statistics: 3,869 death/50,333 diagnosis=7.69%) than the rest of China. What are the new findings?Based on our analysis, the number of infected people in Wuhan is estimated to be 143,000 (88,000 to 242,000) in late January and early February, significantly higher than the published number of diagnosed cases. What are the recommendations for policy and practice?Increased awareness of the original infection rates in Wuhan, China is critically important for proper public health measures at all levels, as well as to eliminate panic caused by overestimated mortality rate that may bias health policy actions by the authorities
ABSTRACT
AIM: To study the effect of the overexpression of p16 on an anion exchange function of band 3 in HeLa cells. METHODS: The expression of p16 and band 3 in HeLa cells was detected by immunohistochemistry (IHC). The p16 cDNA was subcloned to plasmids pEGFP-C1 by PCR and identified by restriction enzyme digestion and sequencing, and then, the recombinant pEGFP-C1-p16 plasmids were transiently transfected into HeLa cells. The expression of fusion protein in HeLa cells was detected by fluorescence microscope. 6-methoxy-N-(3-sulfopropyl)-quinolinium(SPQ)fluorescent probes were used to detect the anion exchange function of band 3. RESULTS: P16 and band 3 were expressed in HeLa cells. The amplificated p16 cDNA sequence was the same as the report sequence. The transfective efficacy of pEGFP-C1-p16 was above 60%. The anion exchange function increased after the transfection of pEGFP-C1-p16 plasmids. CONCLUSION: p16 facilitates the anion exchange function of band 3 in HeLa cells.
