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Methods Mol Biol ; 1777: 283-303, 2018.
Article in English | MEDLINE | ID: mdl-29744843

ABSTRACT

Aromatic peptide amphiphiles can form self-supporting nanostructured hydrogels with tunable mechanical properties and chemical compositions. These hydrogels are increasingly applied in two-dimensional (2D) and three-dimensional (3D) cell culture, where there is a rapidly growing need to store, grow, proliferate, and manipulate naturally derived cells within a hydrated, 3D matrix. Biogelx Limited is a biomaterials company, created to commercialize these bio-inspired hydrogels to cell biologists for a range of cell culture applications. This chapter describes methods of various characterization and cell culture techniques specifically optimized for compatibility with Biogelx products.


Subject(s)
Hydrogels/chemistry , Peptides/chemistry , Protein Multimerization , Biocompatible Materials/chemistry , Cell Culture Techniques , Hydrogels/isolation & purification , Microscopy , Peptides/isolation & purification , Spheroids, Cellular
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