The ultrasonic appearances of primary testicular lymphoma / 中华超声影像学杂志

Objective To explore the sonographic features of primary testicular lymphoma(PLT).Methods The sonographic features of 16 PLT patients confirmed by surgery and pathology were retrospectively analyzed.Results Sixteen PLT patients were typed into 3 ultrasonic patterns:①The diffuse homogeneous echo type:2 cases,the ultrasonic features were homogeneous sieve-like hypoechoic echo,the blood flow signal was rich and radially distributed,with interstisial fibrosis inapparently under-microscope.②The diffuse uneven homogeneous echo type:9 cases,the ultrasonic features lobulated or map-like heterogeneous hypoechoic features,the blood flow signal was rich in linear distribution,and interstisial fibrosis proliferated to form fibrous septa under-microscope.③The focal nodular pattern echo type:5 cases,the ultrasonic features were homogeneous hypoechoic nodules,the blood flow signal was few,interstisial fibrosis hyperplasia surrounding the tumor under-microscope.Conclusions Ultrasonic appearances of primary testicular lymphoma are closely related to its pathologic characteristics,the recognization of their relations contributes to improve the diagnostic value of ultrasound.

Establishment of high performance liquid chromatography-tandem mass spectrometry for the detection of serum oleic acid and its application in insulin resistance / 临床检验杂志

Objective@#To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the detection of serum oleic acid (OA), and preliminarily evaluate the role of OA in insulin resistance (IR) of type 2 diabetes (T2DM). @*Methods@#OA-［ 13 C 5 ］ was used as isotope-labeled internal standard, and the ion pairs of OA and OA-［ 13 C 5 ］ were 281.3/281.3 and 286.3/286.3, respectively. The ultrapure water was used as mobile phase A and methanol: acetonitrile (1∶1, v/v) as mobile phase B in a ZORBAX SB-Aq C18 reversed phase column. Meanwhile, the gradient elution system with a flow rate of 0.3 mL/min was used. According to the CLSI guidelines (EP15-A3), the reliability of the established method was evaluated by detecting the performance indicators such as precision, trueness, linear range, stability and carrying contamination rate. Serum OA levels were detected by the established HPLC-MS/MS method in 109 patients with clinically diagnosed T2DM and 100 healthy controls. The insulin resistance index (HOMA-IR) was calculated to evaluate IR, and the relationship between OA and IR was further analyzed. @*Results@#The established HPLC-MS/MS method for the detection of serum OA had good specificity and linearity in the range of 10-1 000 μmol/L (y=0.007 55x+0.004 83,r=0.997 7), and the low limit of quantification (LLOQ) was 10 μmol/L. It also had good precision, and the within-run coefficient of variation (CV) and total CV were not more than 1.62% and 1.73%, respectively, indicating that the method was suitable for the detection of serum OA. The serum OA levels in T2DM patients ［(425.58 ± 220.17) μmol/L］ were significantly higher than that in the healthy controls ［(113.20±58.00) μmol/L］, and serum OA levels were significantly correlated with HOMA-IR in T2DM patients and healthy controls. The area under the receiver operating characteristic (ROC) curve (AUC) of OA for the diagnosis of IR was 0.689. When the cut-off value identified by Youden index was 235.8 μmol/L, the sensitivity and specificity were 70.4% and 63%, respectively. When OA combined with fasting blood glucose (FBG) to diagnose IR, the AUC increased to 0.806, which was significantly higher than that of OA (P＜0.05). @*Conclusion@#A scientific and efficient HPLC-MS/MS method for the quantitative detection of serum OA is established successfully, which provides a reliable method for the dynamic monitoring of the changes of OA levels in the patients with metabolic diseases.

The ultrasonic appearances of primary testicular lymphoma / 中华超声影像学杂志

Objective@#To explore the sonographic features of primary testicular lymphoma(PLT).@*Methods@#The sonographic features of 16 PLT patients confirmed by surgery and pathology were retrospectively analyzed.@*Results@#Sixteen PLT patients were typed into 3 ultrasonic patterns: ①The diffuse homogeneous echo type: 2 cases, the ultrasonic features were homogeneous sieve-like hypoechoic echo, the blood flow signal was rich and radially distributed, with interstisial fibrosis inapparently under-microscope. ②The diffuse uneven homogeneous echo type: 9 cases, the ultrasonic features lobulated or map-like heterogeneous hypoechoic features, the blood flow signal was rich in linear distribution, and interstisial fibrosis proliferated to form fibrous septa under-microscope. ③The focal nodular pattern echo type: 5 cases, the ultrasonic features were homogeneous hypoechoic nodules, the blood flow signal was few, interstisial fibrosis hyperplasia surrounding the tumor under-microscope.@*Conclusions@#Ultrasonic appearances of primary testicular lymphoma are closely related to its pathologic characteristics, the recognization of their relations contributes to improve the diagnostic value of ultrasound.

Application of animation video in epidural anesthesia in elderly patients with preoperative visit / 中国实用护理杂志

Objective To explore the application effect of postural animation video instruction mode in elderly patients undergoing epidural anesthesia. Methods A total of 300 patients with epidural anesthesia were registered from January 2015 to February 2016. The patients were divided into trial group and control group with 150 cases each. The trial group was preoperatively interviewed by the postural anatomy video instruction mode, and the control group was only preoperatively interviewed by oral interpretation. The epidural anesthesia effect and anxiety between the two groups were compared. Results The difference of anesthesia posture and anatomical position, the average time of epidural puncture, the success rate of one puncture and the degree of anxiety before and after mission were 90.67% (136/150), (14.50 ± 10.20) s, (425.60 ± 78.30) s, 97.33% (146/150) and (21.65 ± 6.62) points respectively. The control group was 22.00%(33/150), (112.60 ± 60.80) s, (589.40 ± 90.70)s, 82.67%(124/150) and (6.65 ± 5.40) points respectively. There were significant differences between the two groups (χ2=8.79, t=13.54-19.88, all P < 0.01). Conclusions The postural anatomical video teaching mode can relieve anxiety, improve the degree of fit of epidural anesthesia and the success rate of puncture, shorten the time of posture, thus shorten the average time of epidural puncture, improve the quality of nursing in the operating room, work efficiency.

Micro-computed tomography and microdissection of the temporal bone of tree shrews.

OBJECTIVE: To understand the morphology and anatomical data of the temporal bone of tree shrews through micro-computed tomography (micro-CT) and microdissection. METHODS: Skull specimens from 10 tree shrews were scanned using micro-CT examination. The acquired images were used for three-dimensional reconstruction and measurement using the Mimics 10.01 software. Twenty tree shrews were subjected to microdissection and the data were measured. RESULTS: Micro-CT and three-dimensional reconstruction could clearly define the three-dimensional spatial position of the ear structure. Micro-CT and microdissection showed that the otic vesicles of the tree shrews were located on both sides of posterior-inferior skull bone. The location of the otic vesicles was superficial, and the bone was thin. All of the structures of the middle and inner ear of the tree shrews were well developed. The ossicular chain was differentiated into the malleus, incus and stapes. The location of the three semi-circular canals of the tree shrews was superficial and easy to dissect. In vivo, the three semi-circular canals were easy to localize and the surface bone was thin. The contour and structure of the cochlea and number of cochlear turns were similar to those in humans. CONCLUSION: This study could provide anatomical data to allow tree shrews to be used as animal models for studying ear diseases.

Mechanosignaling pathways in keloids / 中国组织工程研究

BACKGROUND:Keloid is a very chalenging problem in plastic surgery. Its pathogenesis is very complex, resulting from the combined action of many factors, such as various cytokines, signal transduction pathways, extracelular matrix,etc. At present, a critical role for mechanical force and mechanotransduction in the pathogenesis of keloids has been broadly concerned and becomes the focus of studying the pathogenesis of keloids. OBJECTIVE:To summarize the progress of the mechanosignaling pathway involved in the pathogenesis of keloids in order to further understand the pathogenesis of keloids and provide new ideas for the prevention of keloids. METHODS: The PubMed database and Elsevier database were retrieved for articles published from January 2000 to July 2014 by computer with key words of “keloid, molecular mechanism, mechanical stress, cutaneous scar, mechanobiology, mechanosignaling pathway” in English. A total of 23 articles were included which related to the molecular signal transduction mechanism and mechanosignaling pathway about keloids. RESULTS AND CONCLUSION:The mechanosignaling transduction pathways, such as transforming growth factor-β/Smad, MAPK, integrin, Wnt/β-catenin, RhoA/ROCK and tumor necrosis factor-α/nuclear factor-κB signaling pathway, play an important role in the formation and development of keloids. A number of clinical trials have also shown the effectiveness of a part of mechanosignaling transduction pathway inhibitors in wound healing and reducing scar hyperplasia. The research about mechanosignaling transduction pathways involved in keloids has made some progress, but most stil remain in animal experimental stage. Secondly, various mechanosignaling transduction pathways about correlation and intersectionality stil need further studies to achieve a breakthrough in the prevention of keloids.