Antitumor effects of interleukin-18 gene-modified hepatocyte cell line on implanted liver carcinoma / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the antitumor effects of intrasplenically transplanted interleukin-18 (IL-18) gene-modified hepatocytes on murine implanted liver carcinoma.</p><p><b>METHODS</b>Embryonic murine hepatocyte cell line (BNL-CL2) was transfected with a recombinant adenovirus encoding IL-18 and used as delivery cells for IL-18 gene transfer. Two cell lines, BNL-LacZ and BNL-CL2, were used as controls. One week after intrasplenic injection of C26 cells (colon carcinoma line), tumor-bearing syngeneic mice underwent the intrasplenic transplantation of IL-18 gene-modified hepatocyte cell line and were divided into treatment group (BNL IL-18) and control groups (BNL-LacZ and BNL-CL2). Two weeks later, the serum levels of IL-18, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) in the implanted liver carcinoma-bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T-lymphocytes (CTLs) was measured, and the morphology of the hepatic tumors was studied to evaluate the antitumor effects of the approach.</p><p><b>RESULTS</b>In the treatment group, the serum levels of IL-18, IFN-gamma, TNF-alpha and NO increased significantly. The splenic CTL activity increased markedly (P < 0.01), accompanied by a substantial decrease in tumor volume and the percentage of tumor area and prolonged survival of liver carcinomo-being mice.</p><p><b>CONCLUSIONS</b>In vivo IL-18 expression by ex vivo manipulated cells with IL-18 recombinant adenovirus is able to exert potent antitumor effects by inducing a predominantly T-cell-helper type 1 (Th1) immune response. Intrasplenic transplantation of adenovirus-mediated IL-18 gene-modified hepatocytes could be used as a targeting treatment for implanted liver carcinoma.</p>

Influence of DiKang capsule on expression of TGF? receptor Ⅱ on hepatic satellite cells in rat with liver fibrosis introduced by DMN / 中国免疫学杂志

Objective:To study the influence of DiKang capsule on expression of TGF? receptor Ⅱ on hepatic stellite cells(HSC)in rat with liver fibrosis introduced by DMN.Methods:TGF? receptor Ⅱ on HSC in rat with liver fibrosis introduced by DMN were measured by FCM.Results:In group of 10 ?g/kg DMN injected 3 weeks,expression of TGF? recptor Ⅱ on HSC in these rat fed with DiKang capsule 3 weeks were higher than that not feeding capsule.But in group of injecting 5 ?g/kg DMN continuous 6 weeks,expression of TGF? receptor Ⅱ on HSC does not changed with feeding or not feeding DiKang capsule.Conclusion:DiKang capsule may have a certainly role on expression of TGF? receptor Ⅱ on HSC in rat with liver fibrosis introduced by DMN.

Expression of interleukin-18 in serum,synovial fluid and synovial tissue in patients with rheumatoid arthritis / 中华风湿病学杂志

Objective To investigate the expression of interleukin 18 (IL 18) in serum,synovial fluid and synovial tissue of patients with rheumatoid arthritis (RA) and to identify its pathological role in RA.Methods The serum,synovial fluid and synovial tissue were obtained from patients with RA,and samples from patients with osteoarthritis (OA) and from healthy human were used as control groups.Levels of IL 18 protein in serum and synovial fluid were measured by enzyme linked immunosorbent assay (ELISA).Expression of IL 18 mRNA in synovial tissus was determined by semi quantitative reverse transcriptase polymerase chain reaction (RT PCR).The biologic activity of IL 18 in serum and synovial fluid was detected on the basis of IFN ? secretion from IL 18 responding human myelomonocytic KG 1 cells.Expression of iNOS and COX 2 mRNA in synovial tissue was also determined,and productions of NO and PGE 2 in serum and synovial fluid were measured by enzyme reduction method and ELISA.Results Levels of IL 18 protein and biologic activity of IL 18 in both serum and synovial fluid of patients with RA were significantly increased compared with corresponding samples of the two control groups.In addition,the expression of IL 18 mRNA in synovial tissue of patients with RA was also significantly increased compared with samples of the two control groups.Conclusion The over expressed IL 18 may play an important role in the pathogenesis of RA.

Effects of interferon-? gene-modified hepatocytes on implanted liver carcinoma / 中华消化杂志

Objective To investigate the therapeutic effects of intrasplenically transplanted interferon ? (IFN ?) gene modified hepatocytes on murine implanted liver carcinoma. Methods Murine fetal hepatocytes (BNL?CL2) were transfected with recombinant adenovirus expressing IFN ?. Two cell lines BNL?Lac Z and BNL?CL2 were taken as control. One week after intrasplenically injected C26 cells(colon carcinoma line), sixty tumor bearing syngeneic mice were intrasplenically transplanted IFN ? gene modified hepatocytes and were divided into treated group (BNL?IFN ?) and two control groups (BNL?Lac Z and BNL?CL2), two weeks later, levels of IFN ?, tumor necrosis factor alpha(TNF ?) and nitric oxide(NO) in the serum of liver implanted carcinoma bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T lymphocyte (CTL) was measured. Mophology of hepatic tumors were studied. The therapeutic effects on the mice with the implanted liver carcinoma were also evaluated. Results In treated group (compared with control groups), the levels of IFN ?, TNF ? and NO in the serum increased significantly ( P