Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 14 de 14
Filter
1.
Orv Hetil ; 160(51): 2007-2011, 2019 Dec.
Article in Hungarian | MEDLINE | ID: mdl-31838860

ABSTRACT

Otosclerosis is a bone remodeling disorder affecting exclusively the human temporal bone which causes small bony lesions in the otic capsule. The symptoms depend on the location and the extent of the otosclerotic foci. Hence, clinically the most relevant sign is the conductive hearing loss due to the stapedial otosclerosis with fixation of the stapes footplate. In many cases, the specific anamnestic features, the age of presentation and usually the absence of tympanic membrane pathology can provide a strong clinical suspicion for otosclerosis. Although audiometric and imaging examinations and VEMP testing can confirm our preoperative diagnosis, the histolopathologic examination of the removed stapes footplate is the most accurate way to determine the diagnosis. Orv Hetil. 2019; 160(51): 2007-2011.


Subject(s)
Hearing Loss, Conductive/etiology , Otosclerosis/diagnosis , Stapes/pathology , Audiometry , Humans , Stapes Surgery
2.
Orv Hetil ; 160(41): 1639-1643, 2019 Oct.
Article in Hungarian | MEDLINE | ID: mdl-31587577

ABSTRACT

The endoscopic middle ear surgery as a new technique has been introduced during the past few years in our country and it is available in only a few hospitals. Beyond the lack of external incision, endoscopic transcanal approach provides wide field of view to previously hidden middle ear spaces compared to the traditional microscopic technique. In this case report, we present an endoscopic surgery of middle ear capillary haemangioma that is a rare entity in tympanic cavity, therefore little has been published in the literature. Generally, these kind of vascular tumours occur in the internal auditory canal or in the perigeniculate ganglion area while this lesion originated from the inner surface of the inferior part of the bony tympanic ring. We discuss the difficulties in differential diagnosis and imaging tests then the treatment options. Orv Hetil. 2019; 160(41): 1639-1643.


Subject(s)
Ear Neoplasms/surgery , Ear, Middle/diagnostic imaging , Hemangioma/surgery , Tympanoplasty/methods , Ear Neoplasms/pathology , Endoscopy , Female , Hemangioma/pathology , Humans , Middle Aged , Treatment Outcome
3.
Orv Hetil ; 159(30): 1215-1220, 2018 Jul.
Article in Hungarian | MEDLINE | ID: mdl-30032667

ABSTRACT

Otosclerosis can be found exclusively in the human otic capsule of the temporal bone. Its etiology is still unknown. In the past decades, several potential etiopathogenetic factors have been revealed, however, most studies were based on otosclerotic patients diagnosed by clinical symptoms only. The current experience indicates that one third of this group suffer from non-otosclerotic stapes fixation. In our experimental series, we have diagnosed and classified otosclerotic patients based on histologic examination, and analyzed also the pathogenetic factors. Recent data demonstrate that measles virus and rs1800472 SNP of transforming growth factor beta 1 (TGFß1) gene are marked obvious etiologic factors, which have no therapeutic consequences so far. Furthermore, we summarize the genetic and environmental factors to be found in the literature, which may play a fundamental role in the pathogenesis of otosclerosis. Orv Hetil. 2018; 159(30): 1215-1220.


Subject(s)
Otosclerosis/metabolism , Otosclerosis/virology , RNA, Messenger/metabolism , Stapes/metabolism , Stapes/virology , DNA, Viral/genetics , Female , Humans , Male , Measles/metabolism , Measles/virology , Tumor Necrosis Factor-alpha/metabolism
4.
Ear Nose Throat J ; 97(4-5): E13-E17, 2018.
Article in English | MEDLINE | ID: mdl-29940687

ABSTRACT

Langerhans cell histiocytosis (LCH) is regarded as a clonal disease, usually carrying the activating BRAF mutation V600E. Although LCH theoretically may affect all types of human tissue and typically appears during childhood, temporal bone involvement in adult patients is exceedingly rare. We report an atypical case of a 56-year-old man as one of the oldest patients diagnosed with temporal bone involvement of a BRAF-negative LCH, which caused painless otorrhea and hearing loss. Cutaneous manifestation (multifocal multisystem LCH, also known as Letterer-Siwe disease) was later recognized incidentally. Administration of ifosfamide plus doxorubicin and mesna with supplementary irradiation resulted in a temporary remission followed by slow but constant tumor progression, which after 2.5 years became resistant to treatment with cytarabine and dasatinib. Attempts at therapy (cladribine) to achieve cure are ongoing.


Subject(s)
Hearing Loss/etiology , Histiocytosis, Langerhans-Cell/complications , Histiocytosis, Langerhans-Cell/pathology , Histiocytosis, Langerhans-Cell/therapy , Humans , Male , Middle Aged , Temporal Bone/pathology
6.
Eur Arch Otorhinolaryngol ; 273(1): 63-72, 2016 Jan.
Article in English | MEDLINE | ID: mdl-25559466

ABSTRACT

This prospective case review was performed with the aim to compare and asses the diagnostic values of cone-beam computed tomography (CBCT) and high-resolution computed tomography (HRCT) in the preoperative evaluation of otosclerosis. A total of 43 patients with histologically confirmed stapedial otosclerosis, who underwent unilateral stapedectomies were analyzed. Preoperative temporal bone CBCT and HRCT scans were performed in all cases. Both CBCT and HRCT imaging were characterized by a slice thickness of 0.4-0.625 mm and multiplanar image reconstruction. Histopathologic examination of the removed stapes footplates was performed in all cases. Findings of CBCT and HRCT were categorized according to the modified Marshall's grading system (fenestral or retrofenestral lesions). Histopathologic results were correlated with multiplanar reconstructed CBCT and HRCT scans, respectively. Negative control groups for CBCT (n = 36) and HRCT (n = 27) examinations consisted of patients, who underwent CBCT imaging due to various dental disorders or HRCT analysis due to idiopathic sudden sensorineural hearing loss. Histologically active foci of otosclerosis (n = 31, 72 %) were identified by both CBCT and HRCT in all cases with a sensitivity of 100 %. However, CBCT could not detect histologically inactive otosclerosis (n = 12, 23 %; sensitivity 0 %). In contrast, HRCT showed inactive otosclerosis with a sensitivity of 59.3 %. According to CBCT results, no retrofenestral lesions were found and the overall sensitivity for hypodense lesions was 61.37 %. In conclusion, CBCT is a robust imaging method in the detection of histologically active fenestral hypodense foci of otosclerosis with high sensitivity and radiologic specificity. In the light of these results, HRCT still remains the basic imaging method in the preoperative diagnosis of otosclerosis, since it has much greater sensitivity and specificity in the detection of retrofenestral hypodense lesions and histologically inactive otosclerotic foci in the oval window niche.


Subject(s)
Cone-Beam Computed Tomography , Otosclerosis/diagnostic imaging , Stapes/diagnostic imaging , Tomography, X-Ray Computed/methods , Adolescent , Adult , Female , Humans , Male , Middle Aged , Preoperative Care , Prospective Studies , Sensitivity and Specificity , Stapes/pathology , Stapes Surgery , Young Adult
7.
Eur Arch Otorhinolaryngol ; 271(7): 2083-9, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24609650

ABSTRACT

Tuberculosis remains one of the most challenging infectious diseases, which rarely manifests in the middle ear cleft exclusively. Typical symptoms of tuberculosis have become more and more confusing due to the genetic evolution of different Mycobacterium species. In the diagnosis of tuberculous otitis media (TOM), clinical suspicion plays a fundamental role, when topical and/or systemic antibiotic treatment cannot lead to improvement in ear discharge and inflammation. If there is no other reason of persisting otorrhea, microbiological sampling and culturing are the subsequent steps of diagnosis. These investigations, however, have low sensitivity; therefore a canal wall-up mastoidectomy is recommended, which includes the removal of necrotic bone and multiple histological sampling from various locations. Currently, histopathological analysis is the most robust and reliable method in the diagnosis of TOM. Tuberculin skin test, Mycobacterium-specific PCR and interferon-gamma release assay cannot distinguish between active, inactive or post-infective conditions. According to these considerations, these methods may serve as supplementary assays for the final diagnosis. Having the appropriate diagnosis after surgical intervention and laboratory analysis, medical management should be continued by anti-tuberculosis chemotherapy. Hereby, we demonstrate two cases with primary TOM and provide an overview of the literature in the light of diagnostic and therapeutic guidelines in the management of TOM.


Subject(s)
Antitubercular Agents/therapeutic use , Otitis Media/microbiology , Otitis Media/pathology , Tuberculosis/diagnosis , Tuberculosis/drug therapy , Adult , Female , Humans , Otitis Media/therapy , Young Adult
8.
Otol Neurotol ; 35(6): 1058-64, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24643032

ABSTRACT

BACKGROUND/HYPOTHESIS: Otosclerosis is a frequent cause of hearing impairment characterized by abnormal resorption and deposition of bone in the human otic capsule. It is a disease of complex etiopathogenesis that is caused by both environmental and genetic factors. The goal of this study is to replicate association for genes that were previously reported to be associated with otosclerosis. However, in this study, patients were used in which the presence of otosclerotic foci was confirmed by histologic investigation, in contrast to previous studies, that did not use histologic confirmation. METHODS: Case-control association study using 153 cases and 300 controls. Thirteen single nucleotide polymorphisms (SNPs) in 6 genes (COL1A1, TGFB1, BMP2, BMP4, AGT, and RELN) were genotyped. RESULTS: An association between TGFB1 (rs1800472) and otosclerosis was detected, confirming several previous reports. It is surprising that no association was found between RELN and otosclerosis because the current analysis had very reasonable power and the RELN association has been published before in different articles using several independent populations. CONCLUSION: Our findings strengthen the association of TGFB1 (rs1800472) with otosclerosis. The fact that other genes did not replicate could be due to different reasons like lack of power (BMP2 and BMP4) and possible false-positive initial association (COL1A1 and AGT). A plausible explanation for the lack of association for RELN is that RELN could be associated with a specific otosclerosis-like phenotype that is different from the histologically confirmed phenotype of the patients in this study, and that is clinically not distinguishable.


Subject(s)
Cell Adhesion Molecules, Neuronal/genetics , Extracellular Matrix Proteins/genetics , Nerve Tissue Proteins/genetics , Otosclerosis/genetics , Otosclerosis/pathology , Serine Endopeptidases/genetics , Transforming Growth Factor beta1/genetics , Adult , Angiotensinogen/genetics , Bone Morphogenetic Protein 2/genetics , Bone Morphogenetic Protein 4/genetics , Case-Control Studies , Collagen Type I/genetics , Collagen Type I, alpha 1 Chain , Female , Genetic Predisposition to Disease/genetics , Genotype , Humans , Male , Middle Aged , Phenotype , Polymorphism, Single Nucleotide , Reelin Protein , Young Adult
9.
Eur Arch Otorhinolaryngol ; 271(8): 2131-8, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24048411

ABSTRACT

This retrospective case review was performed with the aim to asses the value of cone-beam computed tomography (CBCT) in the preoperative diagnosis of otosclerosis. A total of 32 patients with histologically confirmed stapedial otosclerosis, who underwent unilateral stapedectomies were analyzed. Preoperative temporal bone CBCT scans were performed in all cases. CBCT imaging was characterized by a slice thickness of 0.3 mm and multiplanar image reconstruction. Histopathologic examination of the removed stapes footplates was performed in all cases. Findings of CBCT were categorized according to Marshall's grading system (from grade 0 to grade 3). Histopathologic results were correlated to multiplanar reconstructed CBCT scans, respectively. Histologically active foci of otosclerosis (n = 21) were identified by CBCT in all cases with a sensitivity of 100 %. However, CBCT was unable to detect histologically inactive otosclerosis (n = 11, sensitivity = 0 %). According to CBCT scans, no retrofenestral lesions were found and all positive cases were recruited into the grade 1 group indicating solely fenestral lesions at the anterior pole of stapes footplates. In conclusion, CBCT is a reliable imaging method with considerably lower radiation dose than high-resolution CT (HRCT) in the preoperative diagnosis of otosclerosis. These results indicate that CBCT has high sensitivity and specificity in the detection of hypodense lesions due to histologically active otosclerosis.


Subject(s)
Otosclerosis , Preoperative Care/methods , Stapes/pathology , Temporal Bone/diagnostic imaging , Adult , Cone-Beam Computed Tomography/methods , Female , Humans , Male , Middle Aged , Otosclerosis/diagnosis , Otosclerosis/pathology , Otosclerosis/surgery , Reproducibility of Results , Retrospective Studies , Sensitivity and Specificity , Stapes Surgery/methods
10.
ISRN Otolaryngol ; 2013: 479482, 2013.
Article in English | MEDLINE | ID: mdl-23864959

ABSTRACT

Genetic predisposition of otosclerosis has long been suspected, but unclarified. Unique coexpression pattern of measles virus receptor (CD46) splicing isoforms in the human otic capsule is assumed, since otosclerosis is a measles virus-associated organ-specific disease. In order to identify CD46 involved in the pathogenesis of otosclerosis, we used representative groups of histologically diagnosed otosclerotic, nonotosclerotic, and normal stapes footplates (n = 109). Consecutive histopathological examinations and CD46-specific Western blot analysis were performed. Normal and nonotosclerotic stapes footplates showed consistent expression of the conventional c, d, e, f, and l CD46 isoforms. In contrast, four novel isoforms (os1-4) translated as intact proteins were additionally detected in each otosclerotic specimen. The study herein presented provides evidence for the otosclerosis-associated expression pattern of CD46. This finding might explain the organ-specific, virus-associated and autoimmune-inflammatory pathogenesis of otosclerosis. Regarding our current knowledge, this is the first report that confirms the presence of four new disease-specific protein variants of CD46.

11.
Otol Neurotol ; 34(5): 808-15, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23370555

ABSTRACT

INTRODUCTION: Recent studies have reported genetic associations between with single nucleotide polymorphism (SNP) of the several genes of the renin-angiotensin-aldosterone (RAA) system in otosclerosis without the confirmation of RAA system expression in human stapes footplates. There are conflicting results. These results are conflicting because RAA system expression has been attributed exclusively to neural, vascular, and renal tissues, exclusively. MATERIALS AND METHODS: Ankylotic stapes footplates (n = 20), cortical bone fragments (n = 10), and human kidney tissue specimens (n = 10) were processed to hematoxylin-eosin (HE) staining and RAA system-specific immunofluorescent assay (IFA), respectively. RESULTS: Histologic diagnosis of otosclerosis was established in all ankylotic stapes footplates. Histologically active- (n = 13) and inactive (n = 7) foci of otosclerosis were consequently characterized by negative immunoreactions for renin, angiotensin converting enzyme (ACE), angiotensin-II (AT-II), and angiotensin-II receptor (AT-IIR), consequently. In cortical bones, a considerable RAA system expression was observed confirmed in the perivascular bone marrow progenitor cells. Kidney specimens, applied as positive controls, showed intense RAA system-specific immunoreactions. CONCLUSION: Concerning current observations, the 4 studied members of RAA system that did not display active expression were not expressed at protein level in otosclerotic stapes footplates. This phenomenon was independent from the histologic activity of otosclerosis. Between these conditions, the etiologic role of RAA system is questionable in the pathogenesis of otosclerosis.


Subject(s)
Otosclerosis/genetics , Otosclerosis/metabolism , Renin-Angiotensin System/genetics , Stapes/metabolism , Adult , Female , Humans , Male , Middle Aged , Otosclerosis/pathology , Polymorphism, Single Nucleotide/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction/methods , Stapes/pathology , Stapes Surgery/adverse effects , Young Adult
12.
Eur Arch Otorhinolaryngol ; 270(3): 793-804, 2013 Mar.
Article in English | MEDLINE | ID: mdl-22843095

ABSTRACT

To review our current knowledge of the pathologic bone metabolism in otosclerosis and to discuss the possibilities of non-surgical, pharmacological intervention. Otosclerosis has been suspected to be associated with defective measles virus infection, local inflammation and consecutive bone deterioration in the human otic capsule. In the early stages of otosclerosis, different pharmacological agents may delay the progression or prevent further deterioration of the disease and consecutive hearing loss. Although effective anti-osteoporotic drugs have become available, the use of sodium fluoride and bisphosphonates in otosclerosis has not yet been successful. Bioflavonoids may relieve tinnitus due to otosclerosis, but there is no data available on long-term application and effects on sensorineural hearing loss. In the initial inflammatory phase, corticosteroids or non-steroidal anti-inflammatory drugs may be effective; however, extended systemic application may lead to serious side effects. Vitamin D administration may have effects on the pathological bone loss, as well as on inflammation. No information has been reported on the use of immunosuppressive drugs. Anti-cytokine targeted biological therapy, however, may be feasible. Indeed, one study on the local administration of infliximab has been reported. Potential targets of future therapy may include osteoprotegerin, RANK ligand, cathepsins and also the Wnt-ß-catenin pathway. Finally, anti-measles vaccination may delay the progression of the disease and potentially decrease the number of new cases. In conclusion, stapes surgery remains to be widely accepted treatment of conductive hearing loss due to otosclerosis. Due to lack of solid evidence, the place of pharmacological treatment targeting inflammation and bone metabolism needs to be determined by future studies.


Subject(s)
Hearing Loss, Sensorineural/prevention & control , Otosclerosis/drug therapy , Anti-Inflammatory Agents/therapeutic use , Bone Density Conservation Agents/therapeutic use , Cytokines/antagonists & inhibitors , Disease Progression , Early Medical Intervention , Flavonoids/therapeutic use , Hearing Loss, Sensorineural/etiology , Humans , Measles Vaccine/therapeutic use , Otosclerosis/complications , Otosclerosis/virology , Vitamin D/therapeutic use
13.
Acta Otolaryngol ; 132(6): 624-31, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22385409

ABSTRACT

CONCLUSION: This study is the first to establish that bone morphogenetic protein 5 (BMP5) plays a role in the pathogenesis of otosclerosis. These results confirm that elevated expression levels of BMPs, members of the transforming growth factor (TGF)-ß superfamily, contribute to the pathologically increased bone turnover in early, active stages of otosclerosis. OBJECTIVES: Otosclerosis is a complex bone remodeling disorder of the otic capsule, which might be characterized by increased expression of different types of BMPs. TGF-ß and BMP are both members of the TGF-ß superfamily and play a critical role in bone resorption and new bone formation. It has been suggested that BMP and its receptors may be involved in the pathologically increased bone turnover observed in otosclerosis. METHODS: Fifty-one otosclerotic and 16 non-otosclerotic ankylotic stapes footplates were histologically analyzed: conventional hematoxylin-eosin staining and BMP2, 4, 5, and 7specific immunofluorescent assays were performed. Cortical bone fragments (n = 35) and incus specimens (n = 6) were used as negative controls. RESULTS: Active otosclerosis (n = 39) was characterized by increased expression of BMP2, 4, 5, and 7. Inactive cases of otosclerosis (n = 12) were characterized by negative immunoreaction for BMPs. Non-otosclerotic stapes specimens (n = 16) and negative controls (n = 41) showed negligible BMP expression. The BMP expression pattern showed a strong correlation with the histological activity of otosclerosis.


Subject(s)
Bone Morphogenetic Protein 2/biosynthesis , Bone Morphogenetic Protein 4/biosynthesis , Bone Morphogenetic Protein 5/biosynthesis , Bone Morphogenetic Protein 7/biosynthesis , Bone Remodeling/physiology , Otosclerosis/metabolism , Stapes/pathology , Adult , Aged , Biomarkers/metabolism , Female , Humans , Immunohistochemistry , Male , Middle Aged , Otosclerosis/pathology , Otosclerosis/surgery , Prognosis , Stapes/metabolism , Stapes Surgery
14.
Eur Arch Otorhinolaryngol ; 269(9): 2043-51, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22130917

ABSTRACT

Otosclerosis is a complex bone remodeling disorder of the human otic capsule that might be associated with various mutations of A1 and A2 alleles of type-I collagen. The study herein presented, investigates the possibilty of the genetic involvement of type-I collagen in the pathogenesis of histologically confirmed otosclerosis. A total of 55 ankylotic stapes footplates were analyzed. Cortical bone fragments (n = 30), incus (n = 3) and malleus (n = 2) specimens were employed as negative controls. Specimens were divided into two groups. The first group was processed using conventional H.E. hematoxylin-eosin (H.E.) staining and type-I collagen-specific immunofluorescent assay (IFA), while the second group was examined by COL1A1 and A2-specific RT-PCR. Otosclerotic- (n = 31) and non-otosclerotic stapes footplates (n = 9) as well as cortical bones (n = 20), incus (n = 2) and malleus specimens (n = 1) showed normal and quite similar A1 and A2 allele expression confirmed by IFA. RT-PCR analysis revealed normal and consistent mRNA expression of both alleles in each specimen. Expression levels and patterns of COL1A1/A2 alleles did not show significant correlation with the histological diagnosis of otosclerosis. Type-I collagen is a highly conserved structure protein, which plays a fundamental role in the integritiy of various connective tissues. Mutations of A1 and A2 alleles result in serious systemic disorders of the skeleton, tendons and skin. Since otosclerosis is an organ-specific disease, it is difficult to explain its genetic association with type-I collagen. In conclusion, we found no evidence supporting the putative link of COL1A1 and COL1A2 alleles with otosclerosis.


Subject(s)
Collagen Type I/genetics , Otosclerosis/genetics , RNA, Messenger/analysis , Stapes/metabolism , Adult , Aged , Ankylosis/genetics , Case-Control Studies , Collagen Type I/metabolism , Collagen Type I, alpha 1 Chain , Female , Gene Frequency , Genetic Predisposition to Disease , Genotype , Humans , Immunohistochemistry , Male , Middle Aged , Mutation , Otosclerosis/metabolism , Reverse Transcriptase Polymerase Chain Reaction
SELECTION OF CITATIONS
SEARCH DETAIL
...