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1.
Med J Malaysia ; 77(2): 237-240, 2022 03.
Article in English | MEDLINE | ID: mdl-35338633

ABSTRACT

INTRODUCTION: Isolation of SARS-CoV-2-infected individuals is an important COVID-19 pandemic control measure. While most cases have uncomplicated infection, a small proportion of them has developed life-threatening disease. We set up a retrospective study to determine preadmission triaging tool to predict the development of severe COVID-19. MATERIALS AND METHODS: A retrospective study was conducted from 1 October 2020 to 31 January 2021 with enrolment of all SARS-CoV-2 PCR-confirmed persons aged ≥13 years. The disease severity was assessed on admission and daily throughout the hospitalisation. Test-positive individuals were considered as having "severe COVID-19" if they had ≥1 of the following: room air oxygen saturation 30 breaths/minute, signs of severe respiratory distress, or received mechanical ventilation and/or vasopressor therapy. Uni- and multi-variate analyses using SPSS Statistics Ver. 26 were performed. RESULTS: We showed that age ≥ 60 years, BMI ≥ 30.0, presentation on days 7-12 of illness, and ≥1 comorbidity were associated with development of severe COVID-19. A scoring system based on the four variables is a useful COVID-19 risk assessment tool. A total score ≥2 had a sensitivity of 60.9%, specificity of 88.2%, positive predictive value of 37.8% and negative predictive value of 95.0%. CONCLUSION: Development of preadmission triaging tool can help health care providers (HCPs) decide on the placement of test-positive individuals to appropriate isolation facilities according to the risk of developing severe COVID-19.


Subject(s)
COVID-19 , Adolescent , COVID-19/diagnosis , Humans , Middle Aged , Pandemics , Retrospective Studies , SARS-CoV-2 , Triage
2.
Disabil Rehabil ; 44(1): 124-130, 2022 Jan.
Article in English | MEDLINE | ID: mdl-32374189

ABSTRACT

PURPOSE: Translating the Neck Disability Index (NDI) into the Malay language (NDI-M); evaluation of psychometric properties in patients with neck pain. METHODS: The NDI-M was translated according to established guidelines. In the first visit, 120 participants completed the NDI-M, visual analogue scale (VAS) for pain and demographic details. 98 participants returned to complete similar questionnaires and the Global Rating of Change (GRoC) scale. The NDI-M was evaluated for internal consistency, test-retest reliability, content validity, construct validity and responsiveness. RESULTS: The NDI-M demonstrated excellent internal consistency (Cronbach's α = 0.84) and good test-retest reliability (ICC2,1 = 0.79). Content validity was confirmed with no floor or ceiling effects. Construct validity was established revealing three-factor subscales explaining 68% of the total variance. The NDI-M showed a moderate correlation with VAS (Rp = 0.49, p < 0.001). Regarding responsiveness, a moderate correlation between NDI-M change scores and VAS change scores was found (Rp = 0.40, p < 0.001). However, there was no significant correlation between NDI-M with GRoC (Rs = 0.11, p = 0.27). CONCLUSIONS: The NDI-M is a reliable and valid tool to measure functional outcomes in patients with neck pain. It is responsive in detecting changes in pain intensity during a patient's rehabilitation journey.Implications for rehabilitationThe NDI was translated into the Malay language and culturally adapted for Malay-speaking patients with neck pain.The NDI-M demonstrated an excellent level of internal consistency and good test-retest reliability. It demonstrated content and construct validity, with three-factor subscales, and moderate responsiveness for pain intensity.The NDI-M is a reliable, valid and responsive instrument to measure functional limitations in patients with neck pain for rehabilitation.


Subject(s)
Cross-Cultural Comparison , Language , Disability Evaluation , Humans , Malaysia , Neck Pain/diagnosis , Psychometrics , Reproducibility of Results , Severity of Illness Index , Surveys and Questionnaires , Translations
3.
Neuroscience ; 300: 325-37, 2015 Aug 06.
Article in English | MEDLINE | ID: mdl-26002315

ABSTRACT

The inferior colliculus (IC) receives many corticofugal projections, which can mediate plastic changes such as shifts in frequency tuning or excitability of IC neurons. While the densest projections are found in the IC's external cortices, fibers originating from the primary auditory cortex (AI) have been observed throughout the IC's central nucleus (ICC), and these projections have shown to be organized tonotopically. Some studies have also found projections from other core and non-core cortical regions, though the organization and function of these projections are less known. In guinea pig, there exists a non-core ventrorostral belt (VRB) region that has primary-like properties and has often been mistaken for AI, with the clearest differentiating characteristic being VRB's longer response latencies. To better understand the auditory corticofugal descending system beyond AI, we investigated if there are projections from VRB to the ICC and if they exhibit a different projection pattern than those from AI. In this study, we performed experiments in ketamine-anesthetized guinea pigs, in which we positioned 32-site electrode arrays within AI, VRB, and ICC. We identified the monosynaptic connections between AI-to-ICC and VRB-to-ICC using an antidromic stimulation method, and we analyzed their locations across the midbrain using three-dimensional histological techniques. Compared to the corticocollicular projections to the ICC from AI, there were fewer projections to the ICC from VRB, and these projections had a weaker tonotopic organization. The majority of VRB projections were observed in the caudal-medial versus the rostral-lateral region along an isofrequency lamina of the ICC, which is in contrast to the AI projections that were scattered throughout an ICC lamina. These findings suggest that the VRB directly modulates sound information within the ascending lemniscal pathway with a different or complementary role compared to the modulatory effects of AI, which may have implications for treating hearing disorders.


Subject(s)
Auditory Cortex/cytology , Auditory Cortex/physiology , Auditory Pathways/cytology , Auditory Pathways/physiology , Inferior Colliculi/cytology , Inferior Colliculi/physiology , Acoustic Stimulation , Action Potentials , Animals , Auditory Perception/physiology , Brain Mapping , Electric Stimulation , Guinea Pigs , Neurons/physiology
4.
Nutr Metab Cardiovasc Dis ; 23(4): 366-74, 2013 Apr.
Article in English | MEDLINE | ID: mdl-21920716

ABSTRACT

BACKGROUND AND AIM: We examined the association of fatty acid (FA) composition in serum phospholipids with the features of metabolic syndrome (MetS) and arterial stiffness. METHODS: Korean men (n = 593, 30-79 yrs) were categorized based on the number of MetS risk factors (RFs) and measured for the markers of MetS, serum phospholipid FA composition and brachial-ankle pulse wave velocity (baPWV), an index for the severity of arterial stiffness. RESULTS: Insulin resistance (HOMA-IR), baPWV, LDL size, and adiponectin were significantly altered corresponding to the number of MetS RFs. The proportions of total monounsaturated FA, palmitoleic acid (16:1), oleic acid (18:1ω-9) and dihomo-γ-linolenic acid (DGLA, 20:3ω-6) in serum phospholipids, and DGLA/linoleic acid (LA) (20:3ω-6/18:2ω-6), deta9-desaturase activity (D9D-16: 16:1/16:0 and D9D-18: 18:1ω-9/18:0) significantly increased corresponding to the number of MetS RFs, but D5D (20:4ω-6/20:3ω-6) decreased. baPWV positively correlated with HOMA-IR, palmitic acid (16:0), oleic acid, D6D (18:3ω-6/18:2ω-6), DGLA/LA and D9D-18, and negatively with adiponectin, LDL size, LA, docosahexaenoic acid (DHA, 22:6ω-3) and D5D. Multiple stepwise regression models revealed that baPWV was significantly influenced by systolic blood pressure, age, body weight, triglyceride and LA in serum phospholipids (R(2) = 0.378). Interestingly, baPWV (1419 ± 1 cm/s) and MetS (22%) were highest in individuals with lower proportion of LA (< 12.361%) and higher proportion of DGLA (≥ 1.412%) in serum phospholipid FAs. CONCLUSION: The features of MetS significantly related to serum phosopholipid FA composition. Particularly, arterial stiffness was associated with LA additively together with DLGA. It may suggest a potential benefit of sufficient amounts of LA in serum or in diet can reduce cardiovascular risk.


Subject(s)
Cardiovascular Diseases/complications , Fatty Acids/blood , Metabolic Syndrome/complications , Phospholipids/blood , Vascular Stiffness , Adiponectin/blood , Analysis of Variance , Ankle Brachial Index , Biomarkers/blood , Cardiovascular Diseases/blood , Cardiovascular Diseases/diagnosis , Cardiovascular Diseases/physiopathology , Chi-Square Distribution , Cross-Sectional Studies , Humans , Insulin Resistance , Linear Models , Male , Metabolic Syndrome/blood , Metabolic Syndrome/diagnosis , Metabolic Syndrome/physiopathology , Middle Aged , Predictive Value of Tests , Pulse Wave Analysis , Republic of Korea
5.
Opt Express ; 17(25): 23037-43, 2009 Dec 07.
Article in English | MEDLINE | ID: mdl-20052230

ABSTRACT

An acoustic transient can be generated inside an absorbing tissue as a result of laser-tissue interaction after pulsed laser irradiation. Herein we report a novel application of this physical process, the optoacoustic wave generation in the inner ear and subsequently the induction of basilar membrane vibrations. These laser induced vibrations show a direct correlation to the laser energy and an indirect correlation to the distance from the irradiation focus. Through these characteristics they may be used, in a new generation of cochlear implants, to improve the frequency specific cochlear activation and consequently improve speech perception in hearing impaired patients with residual hearing.


Subject(s)
Acoustic Stimulation/methods , Basilar Membrane/physiology , Hearing/physiology , Photic Stimulation/methods , Animals , Guinea Pigs , Motion , Vibration
6.
Neuroscience ; 154(1): 370-80, 2008 Jun 12.
Article in English | MEDLINE | ID: mdl-18384971

ABSTRACT

The auditory midbrain implant (AMI), which is designed for stimulation of the inferior colliculus (IC), is now in clinical trials. The AMI consists of a single shank array (20 contacts) and uses a stimulation strategy originally designed for cochlear implants since it is already approved for human use and we do not yet know how to optimally activate the auditory midbrain. The goal of this study was to investigate the effects of different pulse rates and phase durations on loudness and pitch percepts because these parameters are required to implement the AMI stimulation strategy. Although each patient was implanted into a different region (i.e. lateral lemniscus, central nucleus of IC, dorsal cortex of IC), they generally exhibited similar threshold versus phase duration, threshold versus pulse rate, and pitch versus pulse rate curves. In particular, stimulation with 100 mus/phase, 250 pulse per second (pps) pulse trains achieved an optimal balance among safety, energy, and current threshold requirements while avoiding rate pitch effects. However, we observed large differences across patients in loudness adaptation to continuous pulse stimulation over long time scales. One patient (implanted in dorsal cortex of IC) even experienced complete loudness decay and elevation of thresholds with daily stimulation. Comparing these results with those of cochlear implant and auditory brainstem implant patients, it appears that stimulation of higher order neurons exhibits less and even no loudness summation for higher rate stimuli and greater current leakage for longer phase durations than that of cochlear neurons. The fact that all midbrain regions we stimulated, which includes three distinctly different nuclei, exhibited similar loudness summation effects (i.e. none for pulse rates above 250 pps) suggests a possible shift in some coding properties that is affected more by which stage along the auditory pathway rather than the types of neurons are being stimulated. However, loudness adaptation occurs at multiple stages from the cochlea up to the midbrain.


Subject(s)
Auditory Brain Stem Implants , Auditory Threshold/physiology , Cochlear Implantation/methods , Loudness Perception/physiology , Neurofibromatosis 2/surgery , Acoustic Stimulation/methods , Humans , Neurofibromatosis 2/physiopathology , Pitch Perception/physiology , Psychophysics , Time Factors
7.
Obesity (Silver Spring) ; 16(4): 784-9, 2008 Apr.
Article in English | MEDLINE | ID: mdl-18239579

ABSTRACT

OBJECTIVE: The relationship of visceral adiposity with adipocytokines and low-density lipoprotein (LDL) particle distribution and oxidation in Asian metabolically obese, normal-weight (MONW) individuals has not been evaluated. We aimed to investigate the association between visceral adiposity and adipocytokines and cardiovascular disease (CVD) risk factors in MONW Korean women with normal glucose tolerance. METHODS AND PROCEDURES: We examined the metabolic characteristics of 135 non-obese (BMI <25 kg/m(2)) women aged 25-64 years. Twenty-five women (BMI <25 kg/m(2) and visceral fat adiposity (VFA) > or =100 cm(2)) were classified as MONW and 25 women (BMI <25 kg/m(2) and VFA <100 cm(2)), pair-matched for age, weight, height, and menopausal status, as control group. Plasma lipid profiles and adipocytokines were evaluated in these two groups. RESULTS: MONW subjects had higher systolic (P < 0.05) and diastolic blood pressure (P < 0.005) and higher concentrations of triacylglycerol (TG) (P < 0.005), insulin (P < 0.01), and free fatty acid (FFA) (P < 0.05) than control subjects. There was no significant difference between two groups in LDL-cholesterol (LDL-C) concentrations; however, MONW subjects had smaller LDL particles (P < 0.01) and higher concentrations of oxidized LDL (ox-LDL) (P < 0.05) compared with controls. Moreover, MONW subjects had higher concentrations of tumor necrosis factor-alpha (TNF-alpha) (P < 0.05), interleukin-6 (IL-6) (P < 0.05) and leptin (P < 0.05), and lower plasma adiponectin concentrations (P < 0.05). Higher intake of saturated fat with lower ratio of polyunsaturated fatty acids (PUFAs) to saturated fatty acids (SFA) and lower fiber intake than normal subjects were found in MONW women. DISCUSSION: We found an unfavorable inflammatory profile and a more atherogenic LDL profile in MONW female subjects even in the absence of a known CVD risk factors. Moreover, MONW consumed more saturated fat and less fiber than the control group.


Subject(s)
Atherosclerosis/metabolism , Cholesterol, LDL/blood , Leptin/blood , Obesity/metabolism , Adiponectin/blood , Adult , Atherosclerosis/epidemiology , Blood Pressure , Body Weight/physiology , Cholesterol, LDL/chemistry , Energy Intake/physiology , Fatty Acids, Nonesterified/blood , Fatty Acids, Unsaturated/blood , Female , Humans , Insulin/blood , Intra-Abdominal Fat/metabolism , Lipoproteins, LDL/blood , Lipoproteins, LDL/chemistry , Middle Aged , Obesity/epidemiology , Particle Size , Risk Factors , Triglycerides/blood
8.
Urol Int ; 79(4): 332-5, 2007.
Article in English | MEDLINE | ID: mdl-18025852

ABSTRACT

AIM: To evaluate the efficacy of intramuscular injection of 75 mg diclofenac sodium and periprostatic nerve block (PPNB) with 1% lignocaine in controlling pain during transrectal ultrasound (TRUS)-guided prostate biopsy. MATERIALS AND METHODS: A total of 120 patients undergoing TRUS-guided prostate biopsies were prospectively enrolled in the study. First, 20 patients did not get any form of analgesia/anesthesia and served as control; next, 20 patients received an intramuscular injection of diclofenac sodium. PPNB with 1% lignocaine was performed in the remaining 80 patients. Pain was assessed using Wong-Baker Faces Pain-Rating Scale (0-10). RESULTS: All three groups of patients were comparable at baseline in terms of age, prostate-specific antigen and final histological diagnosis. The mean pain scores (+/-SD) for control, diclofenac and PPNB groups were 5.10 +/- 3.14, 3.70 +/- 2.36 and 2.24 +/- 1.63, respectively. The difference was statistically significant between control and PPNB (p = 0.001), and diclofenac and PPNB (p = 0.002), but not between the control and diclofenac group (p = 0.120). In addition, the proportion of patients having mild or no pain (defined as pain score

Subject(s)
Diclofenac/administration & dosage , Nerve Block/methods , Pain/prevention & control , Prostatic Neoplasms/diagnostic imaging , Ultrasound, High-Intensity Focused, Transrectal/adverse effects , Aged , Biopsy, Needle , Case-Control Studies , Humans , Injections, Intramuscular , Lidocaine/administration & dosage , Male , Middle Aged , Pain/etiology , Pain Measurement , Patient Satisfaction , Prospective Studies , Prostatic Neoplasms/pathology , Risk Assessment , Treatment Outcome , Ultrasonography , Ultrasound, High-Intensity Focused, Transrectal/methods
9.
J Orthop Surg (Hong Kong) ; 13(2): 203-6, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16131689

ABSTRACT

This is a case report of an extremely rare condition of atlanto-axial subluxation secondary to gouty arthritis, which mimicked rheumatoid arthritis at presentation. Gouty arthritis involving the spine is a rare condition. We highlight a case of gouty arthritis involving the atlanto-axial joint resulting in joint instability, subluxation, and neurological deficit. A 66-year-old obese woman who had a polyarticular disease for the previous 3 years presented with neck pain and progressive neurology. A 2-stage procedure was performed: posterior decompression and occipitocervical fusion followed by further anterior trans-oral decompression. However, after an initial neurological improvement, she succumbed to aspirational pneumonia and septicaemia. Atlanto-axial subluxation caused by gouty arthritis can present in the same way as rheumatoid arthritis. Therefore, the possibility of this as a differential diagnosis should be kept in mind.


Subject(s)
Arthritis, Gouty/complications , Arthritis, Gouty/diagnosis , Arthritis, Rheumatoid/diagnosis , Atlanto-Axial Joint , Joint Dislocations/diagnosis , Aged , Biopsy, Needle , Combined Modality Therapy , Decompression, Surgical/methods , Diagnosis, Differential , Female , Follow-Up Studies , Humans , Immunohistochemistry , Joint Dislocations/etiology , Joint Dislocations/surgery , Magnetic Resonance Imaging/methods , Obesity/physiopathology , Risk Assessment , Spinal Fusion/instrumentation , Spinal Fusion/methods , Treatment Outcome
10.
Med J Malaysia ; 59 Suppl F: 14-8, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15941155

ABSTRACT

Between April 1998 and December 1999, thirty patients with Idiopathic Scoliosis were operated with Multisegmented Hook-Rod System. These patients were operated at the mean age of 16 years and were followed up for a mean of 22.3 months (range 13-34 months). Seven patients had anterior release to increase the curve flexibility followed by second stage posterior instrumentation on the same day. The average operating time for a posterior instrumentation alone and anterior release combined with posterior instrumentation were 270 minutes and 522 minutes respectively. The average blood loss was 2.2 litres for posterior instrumentation alone and 3.3 litres for single day anterior release and posterior surgery. The mean preoperative Cobb's angle was 70 degrees. The mean immediate postoperative and final follow up Cobb's angles were 38 and 42 degrees, which represented an average coronal plane correction of 46.7% and 40.0% respectively. The mean preoperative apical vertebral rotation was 25 degrees, which improved to 15 degrees after the operation. At final follow up, the mean apical vertebra rotation was 20 degrees, which represented a mean apical vertebral rotation correction of 20%. Complications of the procedure included one transient neurological deficit, one infection, one graft site infection and one case of screw cut out. We were able to obtain satisfactory correction of idiopathic scoliosis with the Multisegmented Hook-Rod System.


Subject(s)
Internal Fixators , Scoliosis/surgery , Spinal Fusion/instrumentation , Splints , Adolescent , Adult , Child , Equipment Design , Female , Follow-Up Studies , Humans , Male , Movement/physiology , Retrospective Studies , Scoliosis/physiopathology , Thoracic Vertebrae/surgery , Treatment Outcome
11.
Ann Acad Med Singap ; 32(2): 257-61, 2003 Mar.
Article in English | MEDLINE | ID: mdl-12772532

ABSTRACT

INTRODUCTION: This study aims to assess the usefulness of day 3 (49 to 72 hours) pre-phototherapy total serum bilirubin (TSB) in predicting subsequent significant hyperbilirubinaemia (SHB) and the feasibility of early discharge for term and near-term glucose-6-phosphate dehydrogenase (G6PD) deficient newborns. MATERIALS AND METHODS: This prospective cohort study involved in born G6PD deficient neonates who were > or = 35 weeks and weighted > or = 2000 g at birth. TSB levels and phototherapy requirements in their first two weeks of life were studied. Day 3 pre-phototherapy TSB in the subgroup weighing > or = 2500 g at birth was analysed for its value in predicting subsequent SHB. RESULTS: Of the 129 neonates, 58 (45%) required phototherapy in the first week. Of these, only 4 patients (3.1%) needed phototherapy to be restarted in the second week. Seventy-one (55%) neonates did not require phototherapy at all. In the absence of SHB in the first week, the probability of its development in the second week was zero (95% confidence interval, 0 to 0.057). In the subgroup weighing > or = 2500 g at birth, day 3 pre-phototherapy TSB < or = 154 umol/L predicted no measurable risk of subsequent SHB (sensitivity, 100%; 95% confidence interval, 91.4% to 100%; negative predictive value, 100%; 95% confidence interval, 86.7% to 100%). CONCLUSIONS: G6PD deficient newborns without SHB in their first week of life were at no measurable risk of its development in the second week. Day 3 pre-phototherapy TSB in the subgroup weighing > or = 2500 g was useful for predicting the risk of subsequent SHB. Low-risk infants, thus identified, may be eligible for discharge on or before day 7 of life. Evidence-based early discharge can decrease the social and financial burden of G6PD deficiency in Singapore.


Subject(s)
Bilirubin/blood , Glucosephosphate Dehydrogenase Deficiency/epidemiology , Jaundice, Neonatal/diagnosis , Awards and Prizes , Birth Weight , Cohort Studies , Female , Glucosephosphate Dehydrogenase/blood , Glucosephosphate Dehydrogenase Deficiency/blood , Humans , Incidence , Infant, Newborn , Jaundice, Neonatal/blood , Jaundice, Neonatal/therapy , Male , Neonatal Screening , Patient Discharge , Phototherapy , Predictive Value of Tests , Prospective Studies , Sensitivity and Specificity , Singapore/epidemiology , Time Factors
12.
Anesth Analg ; 96(2): 396-9, table of contents, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12538184

ABSTRACT

We conducted a prospective, randomized, double-blinded trial comparing preoperative application of EMLA cream and sodium chloride solution dorsal penile block (n = 31) with placebo cream and bupivacaine dorsal penile nerve block (n = 32) for postcircumcision analgesia. Pain was assessed using modified Children's Hospital of Eastern Ontario Pain Scale and the duration of block by the time to requirement of first dose of postoperative analgesic. There was no difference in Children's Hospital of Eastern Ontario Pain Scale between the two groups, but bupivacaine dorsal penile nerve block resulted in longer analgesia (P = 0.003). There were no local or systemic complications related to either technique, and there was a very small incidence of vomiting. We conclude that preoperative application of EMLA cream is an effective and simple method to produce postcircumcision analgesia with a very small incidence of adverse effects.


Subject(s)
Anesthetics, Combined/therapeutic use , Circumcision, Male/adverse effects , Lidocaine/therapeutic use , Nerve Block , Pain, Postoperative/drug therapy , Penis/innervation , Prilocaine/therapeutic use , Acetaminophen/therapeutic use , Administration, Topical , Analgesics, Non-Narcotic/therapeutic use , Analgesics, Opioid/therapeutic use , Anesthetics, Combined/administration & dosage , Anesthetics, Combined/adverse effects , Child , Child, Preschool , Double-Blind Method , Fentanyl/therapeutic use , Humans , Lidocaine/administration & dosage , Lidocaine/adverse effects , Lidocaine, Prilocaine Drug Combination , Male , Nerve Block/adverse effects , Pain Measurement/drug effects , Prilocaine/administration & dosage , Prilocaine/adverse effects , Prospective Studies
13.
Med J Malaysia ; 57(3): 340-7, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12440274

ABSTRACT

A cross-sectional study was conducted to determine the prevalence and type of respiratory conditions including asbestos-related diseases among Malaysian asbestos cement workers. The study population consisted of 1164 workers who had undergone medical surveillance from 1995 to 1997, including full history, physical examination, chest radiography and spirometry. More than half the male workers were smokers or ex-smokers, with smokers having more respiratory symptoms and signs, and reduced FEV1 compared with non smokers. The five most common respiratory conditions diagnosed were bronchial asthma, chronic bronchitis, pulmonary tuberculosis, upper respiratory tract infections and allergic rhinitis. On follow-up, there were also two cases of asbestosis and one case of bronchial carcinoma. The asbestosis cases were probably related to heavy occupational exposure to asbestos fibres in the past, before governmental regulations were gazetted in 1986. Further follow-up is essential for continued monitoring of the health status of asbestos workers.


Subject(s)
Asbestos/adverse effects , Occupational Diseases/epidemiology , Occupational Diseases/etiology , Occupational Exposure/adverse effects , Respiratory Tract Diseases/epidemiology , Respiratory Tract Diseases/etiology , Adolescent , Adult , Female , Humans , Malaysia/epidemiology , Male , Middle Aged
14.
Mol Cell Biol ; 21(15): 5071-81, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11438663

ABSTRACT

Periodic accumulation and destruction of mitotic cyclins are important for the initiation and termination of M phase. It is known that both APC(Cdc20) and APC(Hct1) collaborate to destroy mitotic cyclins during M phase. Here we show that this relationship between anaphase-promoting complex (APC) and Clb proteins is reversed in S phase such that the early Clb kinases (Clb3, Clb4, and Clb5 kinases) inactivate APC(Hct1) to allow Clb2 accumulation. This alternating antagonism between APC and Clb proteins during S and M phases constitutes an oscillatory system that generates undulations in the levels of mitotic cyclins.


Subject(s)
Cyclin B/metabolism , Cyclins/metabolism , Mitosis , S Phase , Saccharomyces cerevisiae Proteins , Ubiquitin-Protein Ligase Complexes , Anaphase-Promoting Complex-Cyclosome , Blotting, Northern , Blotting, Western , Cdc20 Proteins , Cdh1 Proteins , Cell Cycle Proteins/metabolism , Flow Cytometry , Fungal Proteins/metabolism , Gene Deletion , Ligases/metabolism , Microscopy, Fluorescence , Models, Biological , Mutation , Phosphorylation , Plasmids/metabolism , Protein Binding , Time Factors , Transcription, Genetic , Ubiquitin-Protein Ligases , Yeasts/metabolism
15.
Acta Otolaryngol ; 121(3): 398-402, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11425208

ABSTRACT

It has been suggested that the formation and growth of nasal polyp require the remodeling of extracellular matrix. Proteoglycans (PGs) are major components of the extracellular matrix that maintain the integrity of structural tissue. The leucine-rich repeat PGs include lumican, decorin and biglycan and have many important biologic activities in various pathologic conditions, including the remodeling of the extracellular matrix. Therefore, these small-PG families may be involved in the formation and growth of nasal polyp. In the present study, surgical specimens of nasal polyps and nasal mucosa were assessed for expression of mRNA coding for lumican, decorin and biglycan using reverse transcriptase-polymerase chain reaction followed by dot blot hybridization. Lumican, decorin and biglycan mRNA were expressed in all tissue samples examined. Semiquantitative dot blot hybridization revealed that the levels of the lumican and biglycan messages are lower in nasal polyp tissues than in nasal mucosa. The decorin messages in nasal polyp were expressed at levels similar to those in nasal mucosa. These results suggest that lumican, decorin and biglycan may be important components of the extracellular matrix in nasal mucosa. Considering the function of these PGs, normal levels of decorin associated with low levels of biglycan and lumican may play a role in the pathogenesis of nasal polyposis.


Subject(s)
Chondroitin Sulfate Proteoglycans/genetics , Keratan Sulfate/genetics , Nasal Mucosa/pathology , Nasal Polyps/genetics , Nose Neoplasms/genetics , Proteoglycans/genetics , RNA, Messenger/genetics , Adult , Biglycan , Decorin , Extracellular Matrix Proteins , Female , Gene Expression Regulation, Neoplastic/physiology , Humans , Immunoblotting , Lumican , Male , Middle Aged , Nasal Polyps/pathology , Nose Neoplasms/pathology , Reference Values
16.
J Vasc Res ; 38(3): 228-36, 2001.
Article in English | MEDLINE | ID: mdl-11399895

ABSTRACT

Spontaneously hypertensive rats (SHRs) have elevated numbers of apoptotic cells. However, the extent and pattern of cell death at the microvascular level is unexplored. We developed a technique to determine early forms of cell death in vivo in the mesentery by use of the life/death indicator ethidium bromide (EB). The mesenteric microvasculature was superfused with 5 microM EB for a period of 3 min, rinsed and immediately viewed by digital fluorescence microscopy. EB-positive cell structures were observed both in the wall of microvessels as well as in the tissue parenchyma. The microvessels had about 2--4 EB-positive cell structures per 100 microm of vessel length. Larger arterioles (>25 microm) in the SHR had an increased EB-positive structure density. After normalization of the blood pressure in the SHR with adrenalectomy, no significant differences remained between Wistar-Kyoto (WKY) rats and SHRs. After dexamethasone treatment, the adrenalectomized SHRs had a higher EB-positive cell density in the smaller class of microvessels than the WKY rats. In addition, EB-positive cell fragments (0.5--2 microm) were observed in the mesentery microvessel wall, and with TUNEL labeling, they were demonstrated to represent DNA fragments. The percentage of microvessels with EB-positive fragments was higher in the SHR arterioles and capillaries. Capillaries and larger venules (>30 microm) in the SHR had higher levels of cell fragments per vessel length. After adrenalectomy, no significant differences remained between WKY rats and SHRs in any of the microvessel categories. When adrenalectomized rats were treated with dexamethasone, a higher number of EB-positive fragments was detected in the wall of SHR capillaries. These results indicate that the mesentery microcirculation in both strains is subject to an early and nonuniform pattern of cell death, as detected by EB, but is enhanced in selected individual microvascular segments of the SHR by a glucocorticoid-driven mechanism.


Subject(s)
Apoptosis , Hypertension/pathology , Microcirculation/pathology , Adrenalectomy , Animals , Blood Pressure , Cell Death , Cell Nucleus/ultrastructure , Dexamethasone/pharmacology , Ethidium/chemistry , Fluorescent Dyes/chemistry , Glucocorticoids/pharmacology , In Situ Nick-End Labeling , Male , Microcirculation/ultrastructure , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Splanchnic Circulation
17.
Singapore Med J ; 42(2): 82-4, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11358198

ABSTRACT

Fracture-dislocation of the lumbo-sacral spine was an unusual injury and was divided into anterior, posterior and lateral types depending on the displacement of the cephalad portion of the spine over the caudal portion. According to the authors' knowledge, only 31 cases of traumatic fracture-dislocation of the lumbo-sacral spine were reported in the English literature. Only 3 previous reports referred to this injury with a posterior displacement, which was an even rarer injury. This was the fourth report of this type of injury.


Subject(s)
Fractures, Comminuted/diagnostic imaging , Fractures, Comminuted/surgery , Joint Dislocations/diagnostic imaging , Joint Dislocations/surgery , Lumbar Vertebrae/injuries , Sacrum/injuries , Spinal Fractures/diagnostic imaging , Spinal Fractures/surgery , Accidents, Occupational , Accidents, Traffic , Adult , Biomechanical Phenomena , Fractures, Comminuted/etiology , Humans , Joint Dislocations/etiology , Male , Paraplegia/etiology , Spinal Fractures/etiology , Spinal Fusion , Tomography, X-Ray Computed
18.
Med J Malaysia ; 56 Suppl C: 41-5, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11814248

ABSTRACT

The Pedriolle torsion meter is an established method of vertebral rotation assessment in scoliosis. However, the assessment of scoliosis by this method is static and indirect. The objective of this study is to compare the accuracy of a direct method of assessing scoliosis rotation by fluoroscopy compared to the Pedriolle torsion meter. Secondly, to determine that vertebral body rotation changes with supine posture compared to erect position. Eight volunteers with idiopathic scoliosis were assessed for the apical vertebral rotation with this method and the Pedriolle torsion meter. These patients were also assessed in the supine and erect position with the fluoroscopic method to determine if the apical vertebral rotation would change with posture. The mean Cobb angle of the curves was 62.8 degrees (range 45 degrees to 86 degrees). The mean apical vertebral rotation in a standing position was assessed to be 21.5 degrees by Pedriolle torsion meter and 29 degrees by the fluoroscopic method. This difference was not statistically significant by the student t-test. In most patient, the rotation of vertebrae improved by a varying degree ranging from none to 24 degrees in the supine position. In conclusion, the fluoroscopic method is an alternate mean of measuring vertebrae rotation in idiopathic scoliosis, with comparable accuracy to the Pedriolle torsion meter method. The amount of vertebral rotation changes with posture of the patient.


Subject(s)
Rotation , Scoliosis/diagnostic imaging , Scoliosis/physiopathology , Spine/diagnostic imaging , Spine/physiopathology , Adolescent , Child , Female , Fluoroscopy , Humans , Pliability , Posture/physiology , Reproducibility of Results , Torsion Abnormality/diagnostic imaging , Torsion Abnormality/physiopathology
19.
Med J Malaysia ; 56 Suppl C: 61-5, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11814252

ABSTRACT

Neurotization of the brachial plexus is an established procedure in the upper limb. However, neurotization of the lower limb remains experimental. Brunelli reported the use of the ulnar nerve to neurotize the lower limb. Zhao et al reported the use of intercostals nerve to neurotize the lower limb in rats. The aim of the study was to determine the feasibility of using intercostals nerve to neurotize the femoral nerve in human cadavers and to ascertain the ideal intercostals nerve that has the anatomical course that suit this role. Six fresh cadaveric dissection were performed through an extensile midline incision in the postmortem room and the lower six (T7-T12) were identified and traced from their origin. Their length and course identified and charted. T9 and T10 intercostal nerve was thought to be the most suitable nerve as the donor nerve, and the T11 and T12 intercostal nerve could not be free from its intra-muscular course sufficiently to be use as donor.


Subject(s)
Femoral Nerve/pathology , Femoral Nerve/surgery , Nerve Transfer , Paraplegia/pathology , Paraplegia/surgery , Feasibility Studies , Female , Humans , Intercostal Nerves/pathology , Intercostal Nerves/surgery , Male , Middle Aged
20.
Med J Malaysia ; 56 Suppl C: 76-9, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11814256

ABSTRACT

A 2 year-old Malay girl was admitted to our institution with a chesty cough and breathlessness but later found to have a chronic C1/C2 subluxation for one and half year with tetraplegia. Her cervical cord was decompressed and occipito-cervical fusion performed. Her neurological status improved significantly post-operatively and is able to care for her personal hygiene. The authors believe that the ability of the cervical cord to recover in the paediatric age group is remarkable that surgical option should be considered even when all seen lost. We believe that this is the first report in the literature to support this potential.


Subject(s)
Cervical Vertebrae/injuries , Cervical Vertebrae/surgery , Decompression, Surgical , Joint Dislocations/surgery , Quadriplegia/surgery , Recovery of Function , Cervical Vertebrae/diagnostic imaging , Child, Preschool , Female , Humans , Joint Dislocations/diagnostic imaging , Quadriplegia/diagnostic imaging , Radiography , Time Factors
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