ABSTRACT
Forty-eight Burkholderia isolates from different land use systems in the Amazon region were compared to type strains of Burkholderia species for phenotypic and functional characteristics that can be used to promote plant growth. Most of these isolates (n=46) were obtained by using siratro (Macroptilium atropurpureum - 44) and common bean (Phaseolus vulgaris - 2) as the trap plant species; two isolates were obtained from nodules collected in the field from Indigofera suffruticosa and Pithecellobium sp. The evaluated characteristics were the following: colony characterisation on "79" medium, assimilation of different carbon sources, enzymatic activities, solubilisation of phosphates, nitrogenase activity and antifungal activity against Fusarium oxysporium f. sp. phaseoli. Whole cell protein profiles, 16S rRNA, gyrB, and recA gene sequencing and multilocus sequence typing were used to identify the isolates. The isolates showed different cultural and biochemical characteristics depending on the legume species from which they were obtained. Except for one isolate from I. suffruticosa, all isolates were able to solubilise calcium phosphate and present nitrogenase activity under free-living conditions. Only one isolate from common beans, showed antifungal activity. The forty four isolates from siratro nodules were identified as B. fungorum; isolates UFLA02-27 and UFLA02-28, obtained from common bean plants, were identified as B. contaminans; isolate INPA89A, isolated from Indigofera suffruticosa, was a close relative of B. caribensis but could not be assigned to an established species; isolate INPA42B, isolated from Pithecellobium sp., was identified as B. lata. This is the first report of nitrogenase activity in B. fungorum, B. lata and B. contaminans.
Subject(s)
Biodiversity , Burkholderia/classification , Burkholderia/metabolism , Genetic Variation , Nitrogen Fixation , Plants/microbiology , Soil Microbiology , Bacterial Proteins/analysis , Bacterial Proteins/genetics , Bacterial Typing Techniques , Brazil , Burkholderia/genetics , Burkholderia/isolation & purification , Cluster Analysis , DNA Gyrase/genetics , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Molecular Sequence Data , Multilocus Sequence Typing , Phylogeny , RNA, Ribosomal, 16S/genetics , Rec A Recombinases/genetics , Rhizosphere , Sequence Analysis, DNAABSTRACT
As vias aéreas superiores (VAS) são a principal porta de entrada do organismo. Apresentam uma ampla cobertura mucosa como barreira física aos agentes infecciosos, irritantes ambientais, etc. Há importantes mecanismos imunes específicos e inespecíficos nesta área.
