Association of ionophores, yeast, and bacterial probiotics alters the abundance of ruminal microbial species of pasture intensively finished beef cattle.

The effect of the association of non-protein nitrogen, yeast, and bacterial probiotics on the ruminal microbiome of beef cattle intensively finished on pasture was evaluated. The experiment was carried out in a completely randomized design with five treatments and four replications. The treatments consisted of a group of animals kept on pasture that received low consumption supplementation (LS) and four groups that received for 98 days, 17.5 g concentrate kg-1 body weight. The supplements were composed of the association of additives: urea (U), slow-release non-protein nitrogen (U+SRN), yeast (Saccharomyces cerevisiae; U+SRN+Y), and bacterial probiotics (live strains of bacteria; U+SRN+Y+BP). All supplements also contained salinomycin and virginiamycin. After slaughtering the animals, samples of ruminal content were collected to quantify groups of fibrolytic bacteria (Ruminococcus albus and Fibrobacter succinogenes), non-fibrolytic (Prevotella ruminicola, Selenomonas ruminantium, and Streptococcus bovis), Archaea, and ciliate protozoa, using the qPCR technique. The abundance of F. succinogenes was the same for the LS animals and those that received the supplement U+SRN+Y (1.42×108 copies mL-1) but higher than the other treatments. Supplementation reduced by 90% the abundance of S. bovis compared to the LS. The inclusion of yeast increased the abundance of fibrolytic bacteria by 2.2-fold. For animals that received the supplement U+SRN+Y+BP and the LS, there was no difference for non-fibrolytic bacteria (3.07×109 copies mL-1). The use of yeasts and sources of non-protein nitrogen in high-concentrate diets for beef cattle stimulates the growth of fibrolytic bacteria, which can contribute to the reduction of digestive disorders and metabolic diseases in animals that receive diets with high concentrate in pasture intensive termination systems.

Efeito da aplicação de silicato de cálcio em Brachiaria rizantha cv. Marandu sobre a população de ninfas do percevejo castanho das raízes Scaptocoris carvalhoi Becker, 1967, características químicas do solo, planta e produção de matéria seca / Application effect of calcium silicate in Brachiaria brizantha cv. Marandu on the nymph population of the brown root stinkbug, soil chemical characteristics, and plant and dry matter production

Neste trabalho, objetivou-se avaliar o efeito de diferentes doses de silicato de cálcio sobre a população de ninfas de percevejo castanho das raízes em Brachiaria brizantha, nas características químicas do solo, planta e produção de matéria seca. O experimento foi conduzido em blocos casualizados, com cinco tratamentos e quatro repetições, totalizando 20 parcelas. Os tratamentos testados foram cinco doses (0, 0,5, 1, 2, e 4 t ha-1) de silicato de cálcio aplicadas junto com a adubação NPK na implantação da B. brizantha. Foi avaliado o número de ninfas do percevejo (profundidade de 0 a 40 cm), o pH e os teores de cálcio e de silício no solo. Aos 120 dias do plantio, a gramínea foi cortada, determinando-se a composição química e a produção de massa seca. Verificou-se que a aplicação de 2,6 t ha-1 de silicato de cálcio foi a melhor dosagem estimada para a redução de ninfas do percevejo castanho das raízes. A aplicação de doses crescentes de silicato de cálcio no solo promoveu o incremento do pH, dos teores de cálcio e silício no solo e o aumento na produção de matéria seca de B. brizantha.

The objective of this study was to evaluate the effects of different calcium silicate rates on the nymph population of the brown root stinkbug in B. brizantha cv. marandu, in the soil chemical characteristics, and plant and dry matter production. The experiment was carried out in casualized blocks with five treatments and four repetitions, in a total of 20 plots. The treatments tested were five rates (0, 0.5, 1, 2, and 4 t ha -1) of calcium silicate applied together with NPK fertilization in the implantation of B. brizantha. The nymph number (depths 0 to 40 cm), pH, and soil calcium and silicon content were evaluated. On the 120th day after sowing, the grass was cut to determine the chemical composition and dry matter production. It was verified that the application of 2.6 t ha -1 of calcium silicate was the best estimated rate for the reduction of brown root stinkbug nymphs. The application of increasing doses of calcium silicate in the soil caused an increase of the pH, calcium and silicon content in the soil, and an increase in B. brizantha dry matter production.