ABSTRACT
This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and the catalase and superoxide dismutase supplementation of diluent on parameters of frozen-thawed spermatozoa from epididymis of Nelore bulls: Experiment 1: spermatozoa diluted in Tris-egg yolk with glycerol (3%, 5% or 7%) and stabilisation times (0, 2 or 4 hr at 5°C); Experiment 2: Tris-egg yolk only, Tris-egg yolk with catalase (CAT, 50 or 100 U ml-1 ) or superoxide dismutase (SOD, 50 or 100 U ml-1 ). Frozen-thawed spermatozoa were evaluated for kinetic parameters, plasma membrane and acrosome integrity, mitochondrial activity and IVF capacity. ALH and BCF were affected (p < .05) by glycerol at 3% after 4-hr equilibration time and 7% after 2-hr equilibration time. Glycerol 3% had lower (p < .05) iPM and iAc after 4 hr. Glycerol 5% had greater (p < .05) hPMM after 4 hr and iAc after 2 hr than at 0 hr. SOD 100 U ml-1 had lower (p < .05) linearity and wobble compared to control group. No was observed differences to fertilisation rate (p < .05) among groups. In conclusion, glycerol 5% in Tris-egg yolk extender for 4 hr is suitable for the preservation of sperm kinetics and membrane integrity. CAT (50 and 100 U ml-1 ) or SOD (50-100 U ml-1 ) had no beneficial effects on sperm kinetics, plasma and acrosomal membrane integrity, mitochondrial activity or the capacity for IVF of frozen-thawed spermatozoa from epididymis of Nelore bulls.
Subject(s)
Antioxidants/pharmacology , Cryoprotective Agents/pharmacology , Epididymis/cytology , Glycerol/pharmacology , Insemination, Artificial/veterinary , Semen Preservation/veterinary , Specimen Handling/veterinary , Spermatozoa/drug effects , Animals , Catalase/pharmacology , Cattle , Cryopreservation/veterinary , Freezing/adverse effects , Male , Sperm Motility/drug effects , Superoxide Dismutase/pharmacology , Time FactorsABSTRACT
As alterações anatômicas do aparelho reprodutor masculino de ovinos são pouco relatadas, especialmente a condição de bipartição escrotal. Devido à importância do esclarecimento sobre esta alteração anatômica, objetivou-se relatar o caso de um ovino da raça Morada Nova que apresentava bipartição escrotal associada à má formação prepucial. Observou-se que essa alteração pode ocorrer como condição de adaptabilidade a climas tropicais.
The anatomical changes in the male reproductive system of sheep are rarely described, especially the scrotal bipartition condition. Due to the importance of clarification of this anatomical change, we aimed to describe a case of a Morada Nova sheep that had scrotal bipartition associated with preputial malformation. It was observed that this change may occur as a adaptability condition to tropical climates.
