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1.
Clin Immunol ; 145(3): 177-88, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23103487

ABSTRACT

Evidences indicate that pregnancy can alter the Ag-specific T-cell responses. This work aims to evaluate the impact of pregnancy on the in vitro HIV-1-specific immune response. As compared with non-pregnant patients, lower T-cell proliferation and higher IL-10 production were observed in T-cell cultures from pregnant patients following addition of either mitogens or HIV-1 antigens. In our system, the main T lymphocyte subset involved in producing IL-10 was CD4(+)FoxP3(-). Depletion of CD4(+) cells elevated TNF-α and IFN-γ production. Interestingly, the in vitro HIV-1 replication was lower in cell cultures from pregnant patients, and it was inversely related to IL-10 production. In these cultures, the neutralization of IL-10 by anti-IL-10 mAb elevated TNF-α release and HIV-1 replication. In conclusion, our results reveal that pregnancy-related events should favor the expansion of HIV-1-specific IL-10-secreting CD4(+) T-cells in HIV-1-infected women, which should, in the scenario of pregnancy, help to reduce the risk of vertical HIV-1 transmission.


Subject(s)
HIV Infections/complications , HIV Infections/immunology , HIV-1/immunology , Pregnancy Complications, Infectious/immunology , T-Lymphocyte Subsets/immunology , Adult , Case-Control Studies , Female , HIV Antigens/administration & dosage , HIV Infections/transmission , HIV Infections/virology , HIV-1/physiology , Humans , In Vitro Techniques , Infant, Newborn , Infectious Disease Transmission, Vertical/prevention & control , Interleukin-10/biosynthesis , Lymphocyte Activation , Pregnancy , Pregnancy Complications, Infectious/virology , T-Lymphocyte Subsets/virology , T-Lymphocytes, Regulatory/immunology , T-Lymphocytes, Regulatory/virology , Virus Replication/immunology , Young Adult
2.
J Clin Immunol ; 31(2): 186-94, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21136282

ABSTRACT

Our objective was to evaluate the in vitro functional profile of T cells from uninfected neonates born from HIV-1-infected pregnant women who controlled (G1) or not (G2) the virus replication. We demonstrated that the lymphoproliferation of T cell to polyclonal activators was higher in the G2 as compared with G1. Nevertheless, no detectable proliferative response was observed in response to HIV-1 antigens in both neonate groups. Cytokine dosage in the supernatants of these polyclonally activated T cell cultures demonstrated that, while IL-10 was the dominant cytokine produced in G1, Th17-related cytokines were significantly higher in G2 neonates. The higher Th17 phenotype tendency in G2 was related to high production of IL-23 by lipopolysaccharide-activated monocyte-derived dendritic cells from these neonates. Our results demonstrated immunological disorders in uninfected neonates born from viremic HIV-1-infected mothers that can help to explain why some of these children have elevated risk of clinical morbidity and mortality due to pathological hypersensitivity.


Subject(s)
HIV Infections/immunology , HIV-1/immunology , Th17 Cells/immunology , Adult , Cell Proliferation , Cells, Cultured , Cytokines/blood , Cytokines/immunology , Dendritic Cells/immunology , Female , Fetal Blood/cytology , HIV Seropositivity/immunology , Humans , Infant, Newborn , Leukocytes, Mononuclear/immunology , Lymphocyte Count , Pregnancy , T-Lymphocytes/cytology , T-Lymphocytes/immunology , Th1 Cells/immunology , Young Adult
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