Fluorescence spectroscopy determination of fluoroquinolones by charge-transfer reaction.

The charge-transfer (CT) reaction between chloranilic acid (CL) as a pi-electron acceptor and lomefloxacin (LOM), fleroxacin (FLX), ciprofloxacin (CPFX), norfloxacin (NOR) as electron donor have been studied by fluorimetry. The CT complexes have stable purple color in acetone solution and the fluorescence intensity of the CT complexes was enhanced in 4-14 fold higher than that of the four fluoroquinolones (FQS) itself, therefore a new spectrofluorimetric method with simple, rapid, accurate, high sensitivity and good selectivity for determination of the four FQS has been developed. The method was applied for determination of drugs (LOM, FLX, CPFX and NOR) in tablets with mean percentage accuracies 99.80+/-1.12, 99.93+/-0.92, 99.23+/-1.36 and 99.87+/-0.81, respectively.

Fluorescence Property of Reaction Productsof Sparfloxacin with Halide and Its Application / 分析化学

The fluorescence characteristics of reaction products of sparfloxacin with halide (F, Cl, Br, I) have been studied. It was found that sparfloxacin was oxidized by nitrous acid then reacted with halide further to formstrong fluorescence substances in acid media. Their fluorescence intensitites enhanced with aggrandizing of atom radii of halides, which could emit the strong fluorescence 58～151 fold more than that of sparfloxacin itself. Thus a new sensitive method for the direct determination of sparfloxacin in human urine by derivative-synchronous fluorescence was presented. A plausible mchanism was proposed to explin this behavior.