ABSTRACT
BACKGROUND: The aggressive and refractory extranodal natural killer/T-cell lymphoma, nasal type (ENKTL-NT) is a subtype of non-Hodgkin's lymphoma. Succinylation promotes progression in a variety of tumors, but its mechanism in ENKTL-NT is unclear. METHODS: Bioinformatic analysis was performed to screen differentially expressed genes in the ENKTL dataset. Cell transfection techniques were used for knockdown and overexpression of genes. The mRNA and protein expression were detected using RT-qPCR and western blot, respectively. Immunohistochemical staining was used to assess protein expression in situ. For the detection of cell proliferation activity, CCK-8, clonal formation, and EDU staining assays were used. Flow cytometry was employed to detect apoptosis. Co-immunoprecipitation was utilized for the identification of protein interactions and succinylation modifications. RESULTS: Succinyltransferase CPT1A was highly elevated in ENKTL-NT and was associated with a dismal prognosis. CPT1A knockdown suppressed SNK-6 cells' proliferation and induced apoptosis, while these effects were reversed by the overexpression of 14-3-3theta. Co-immunoprecipitation results showed that CPT1A caused succinylation of 14-3-3theta at site of K85, thereby enhancing the protein stability. Suppression of CPT1A-induced succinylation of 14-3-3theta by ST1326 resulted in the inhibition of SNK-6 cell proliferation and increased apoptosis. Paclitaxel combined with knockdown of CPT1A significantly inhibited the proliferation of ENKTL-NT compared to paclitaxel alone. CONCLUSION: CPT1A induces succinylation of 14-3-3theta at the K85 site, promoting ENKTL-NT proliferation. The anti-ENKTL activity of paclitaxel was improved when combined with CPT1A knockdown.
ABSTRACT
The development of advanced materials for information encryption with time-dependent features is essential to meet the increasing demand on encryption security. Herein, smart materials with orthogonal and temporal encryption properties are successfully developed based on a dynamic assembly-induced multicolour supramolecular system. Multicolour fluorescence, including blue, orange and even white light emissions, is achieved by controlling the supramolecular assembly of pyrene derivatives by tailoring the solvent composition. By taking advantage of the tuneable fluorescence, dynamically controlled information encryption materials with orthogonal encryption functions, e.g., 3D codes, are successfully developed. Moreover, time-dependent information encryption materials, such as temporal multi-information displays and 4D codes, are also developed by enabling the fluorescence-controllable supramolecular system in the solid phase, showing multiple pieces of information on a time scale, and the correct information can be identified only at a specified time. This work provides an inspiring point for the design of information encryption materials with higher security requirements.
