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J Sep Sci ; 44(12): 2391-2398, 2021 Jun.
Article in English | MEDLINE | ID: mdl-33848379

ABSTRACT

In the study, high-speed counter-current chromatography was used for separation and purification of magnoflorine, spinosin, and 6‴-feruloyspinosin from Ziziphi Spinosae Semen. With n-butanol-ethyl acetate-water (2:3:5, v/v) as the optimum solvent system, about 75 mg of magnoflorine, 110 mg of spinosin, and 40 mg of 6‴-feruloyspinosin were isolated from 0.5 g of crude extract of Z. Spinosae Semen, with the purity of 95.7, 97.2, and 96.4%, respectively. The chemical structures were identified by MS and NMR spectroscopy. In addition, the antidepressant activity of the isolated components was evaluated by PC12 cells injury model and chronic unpredictable mild stress depression mouse model. The results showed that high-speed counter-current chromatography could be used to realize the one-time rapid preparation and separation of magnoflorine, spinosin, and 6‴-feruloyspinosin from Z. Spinosae Semen and compatibility of these isolated components has certain antidepressant activity.


Subject(s)
Aporphines/isolation & purification , Drugs, Chinese Herbal/chemistry , Flavonoids/isolation & purification , Plant Extracts/isolation & purification , Ziziphus/chemistry , Animals , Aporphines/chemistry , Chromatography, High Pressure Liquid , Countercurrent Distribution , Flavonoids/chemistry , Male , Mice, Inbred ICR , PC12 Cells , Plant Extracts/chemistry , Rats
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