Bio-butanol production from glycerol with Clostridium pasteurianum CH4: the effects of butyrate addition and in situ butanol removal via membrane distillation.

BACKGROUND: Clostridium pasteurianum CH4 was used to produce butanol from glycerol. The performance of butanol fermentation was improved by adding butyrate as the precursor to trigger the metabolic pathway toward butanol production, and by combining this with in situ butanol removal via vacuum membrane distillation (VMD) to avoid the product inhibition arising from a high butanol concentration. RESULTS: Adding 6 g L(-1) butyrate as precursor led to an increase in the butanol yield from 0.24 to 0.34 mol butanol (mol glycerol)(-1). Combining VMD and butyrate addition strategies could further enhance the maximum effective butanol concentration to 29.8 g L(-1), while the yield was also improved to 0.39 mol butanol (mol glycerol)(-1). The butanol concentration in the permeate of VMD was nearly five times higher than that in the feeding solution. CONCLUSIONS: The proposed butyrate addition and VMD in situ butanol removal strategies are very effective in enhancing both butanol titer and butanol yield. This would significantly enhance the economic feasibility of fermentative production of butanol. The VMD-based technology not only alleviates the inhibitory effect of butanol, but also markedly increases butanol concentration in the permeate after condensation, thereby making downstream processing easier and more cost-effective.

Enhancing butanol production with Clostridium pasteurianum CH4 using sequential glucose-glycerol addition and simultaneous dual-substrate cultivation strategies.

Adding butyrate significantly enhanced butanol production from glycerol with Clostridium pasteurianum CH4, which predominantly produces butyrate (instead of butanol) when grown on glucose. Hence, the butyrate produced from assimilating glucose can be used to stimulate butanol production from glycerol under dual-substrate cultivation with glucose and glycerol. This proposed butanol production process was conducted by employing sequential or simultaneous addition of the two substrates. The latter approach exhibited better carbon source utilization and butanol production efficiencies. Under the optimal glucose to glycerol ratio (20 g L(-1) to 60 g L(-1)), the simultaneous dual-substrate strategy obtained maximum butanol titer, productivity and yield of 13.3 g L(-1), 0.28 g L(-1) h(-1), and 0.38 mol butanol/mol glycerol, respectively. Moreover, bagasse and crude glycerol as dual-substrates were also converted into butanol efficiently with a maximum butanol concentration, productivity and yield of 11.8 g L(-1), 0.14 g L(-1) h(-1), and 0.33 mol butanol/mol glycerol, respectively.