ABSTRACT
OBJECTIVE: To detect breast cancer specific gene 1 (BCSG1) expression in different breast tissue, analysis its correlation with clinical parameters and evaluate the prognosis of breast cancer. METHODS: The expression of BCSG1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) in surgical specimens from 84 cases of breast disease patients selected randomly at XinHua Hospital affiliated with Shanghai Second Medical University from September 1999 to December 2002. Of 84 cases, 72 case were breast cancer. Statistic analysis BCSG1 gene expression correlation with clinical parameters of breast cancer. 72 breast cancers were followed up (4 - 43 months) to set up independent prognosis factor by survival analysis. RESULTS: BCSG1 was undetectable in all benign breast lesions, while was detectable in 36.1% of all breast cancer samples (26/72), in which 79.2% of stage III/IV cases were positive (19/24). The expression of BCSG1 was tightly correlated with the stage (P = 0.000) and the size of tumor (P = 0.007). Both ER (P = 0.027) and BCSG1 (P = 0.001) were the independent prognosis factor of breast cancer. CONCLUSION: BCSG1 is one of independent tumor marker of breast cancer, the expression of BCSG1 is closely correlated to the stage of breast cancer and the tumor size. Maybe, BCSG1 is a new prognosis factor of breast cancer.
Subject(s)
Breast Neoplasms/genetics , Neoplasm Proteins/genetics , gamma-Synuclein/genetics , Adult , Aged , Aged, 80 and over , Breast Neoplasms/diagnosis , Breast Neoplasms/pathology , Female , Gene Expression , Humans , Middle Aged , Neoplasm Staging , Prognosis , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Expression of myf-5, a key component of myogenic regulatory genes, expands into the ventral marginal zone during Xenopus gastrulation after the dorsal activation takes place. Little is known about how this dynamic expression pattern occurs. Here, we provide evidences to suggest that Activin/Nodal signals participate in the regulation of ventral expression of Xmyf-5 in gastrula embryos. Two Smad binding elements (SBEs) within the Xenopus myf-5 promoter can specifically interact with Smad4 protein. Furthermore, we demonstrate that the two SBEs are both indispensable for conferring responsiveness to Activin/Nodal signals and to ventral expression of myf-5 in Xenopus gastrula embryos.
Subject(s)
Activins/physiology , DNA-Binding Proteins , Gastrula/metabolism , Gene Expression Regulation, Developmental/physiology , Muscle Proteins/genetics , Trans-Activators , Transforming Growth Factor beta/physiology , Xenopus/embryology , Animals , Base Sequence , DNA Primers , Female , Myogenic Regulatory Factor 5 , Nodal Protein , Promoter Regions, Genetic , Xenopus ProteinsABSTRACT
Though the Wnt/beta-catenin signaling pathway is known to play key roles during Xenopus axis specification, whether it signals exclusively through Lef/Tcf transcription factors in this process remains unclear. To investigate this issue, we generated transgenic frog embryos expressing green fluorescent protein (GFP) driven by a Lef/Tcf-dependent and Wnt/beta-catenin-responsive promoter. This promoter is highly sensitive and even detects maternal beta-catenin activity prior to the large-scale transcription of zygotic genes. Unexpectedly, GFP expression was observed only in some, but not all, known Wnt/beta-catenin-positive territories in Xenopus early development. Furthermore, ubiquitous expression of dominant Lef-1 protein variants from transgenes revealed that zygotic Lef/Tcf activity is required for the ventroposterior development of Xenopus embryos. In summary, our results suggest that endogenous Wnt/beta-catenin activity does not result in obligatory Lef/Tcf-dependent gene activation, and that the ventroposteriorizing activity of zygotic Wnt-8 signaling is mediated by Lef/Tcf proteins.
Subject(s)
Body Patterning/genetics , Cytoskeletal Proteins/genetics , DNA-Binding Proteins/genetics , Gene Expression Regulation, Developmental , HMGB Proteins/genetics , High Mobility Group Proteins/genetics , Proto-Oncogene Proteins/genetics , Trans-Activators/genetics , Transcription Factors/genetics , Xenopus/embryology , Xenopus/genetics , Zebrafish Proteins , Animals , Animals, Genetically Modified , Cadherins/genetics , Embryo, Nonmammalian/physiology , Genes, Reporter , Genes, fos , Green Fluorescent Proteins , Leucine Zippers , Luciferases/genetics , Luminescent Proteins/genetics , Lymphoid Enhancer-Binding Factor 1 , NF-E2-Related Factor 1 , Protein-Tyrosine Kinases/genetics , Signal Transduction , TCF Transcription Factors , Transcription Factor 7-Like 1 Protein , Transcription Factor 7-Like 2 Protein , Transcriptional Activation , Wnt Proteins , Xenopus Proteins/genetics , Zygote/physiology , beta CateninABSTRACT
Myf-5, a member of the muscle regulatory factor family of transcription factors, plays an important role in the determination, development, and differentiation of the skeletal muscle. Factors that regulate the expression of myf-5 itself are not well understood. We show here that a T-box binding site in the Xenopus myf-5 promoter mediated the activation of myf-5 expression through specific interaction with nuclear proteins of gastrula embryos. The T-box binding site could be bound by and respond to T-box proteins. T-box genes could induce Xmyf-5. The results suggest that T-box proteins are involved in the specification of myogenic mesoderm and muscle development.
