A Combined Transcriptomic and Proteomic Approach to Reveal the Effect of Mogroside V on OVA-Induced Pulmonary Inflammation in Mice.

Mogroside V is a bioactive ingredient extracted from the natural food Siraitia grosvenorii which possesses functions that stimulate lung humidification and cough relief activities, but its underlying mechanisms were rarely studied. To estimate its potential protective effect on ovalbumin (OVA)-induced pulmonary inflammation and understand its system-wide mechanism, integrated omics was applied in this study. Mogroside V effectively reduced the levels of IgE, TNF-α, and IL-5 in OVA-induced mice. The results of RNA-seq and data-independent acquisition proteomics approach revealed that 944 genes and 341 proteins were differentially expressed in the normal control group (NC) and ovalbumin-induced control group (OC) and 449 genes and 259 proteins were differentially expressed between the OC and the group treated with 50 mg/kg mogroside V (MV). After a combined analysis of the transcriptome and the proteome, 93 major pathways were screened, and we discovered that mogroside V exerts an anti-inflammation effect in the lung via NF-κB and JAK-STAT, both of which are among the signaling pathways mentioned above. In addition, we found that the key regulatory molecules (Igha, Ighg1, NF-κB, Jak1, and Stat1) in the two pathways were activated in inflammation and inhibited by mogroside V. Thus, mogroside V may be the main bioactivity component in S. grosvenorii that exerts lung humidification and cough relief effects.

[Chemical Constituents from Young Fruits of Citrus maxima cv. Shatian].

OBJECTIVE: To investigate the chemical constituents from the young fruits of Citrus maxima cv. Shatian. METHODS: The chemical constituents were isolated and purified by silica gel column chromatography and recrystallization, and their structures were identified on the basis of physicochemical properties and spectral analysis. RESULTS: Seven compounds were isolated and identified as naringenin (I), marmin (II), naringin (III), ß-sitosterol (IV) 5,7-dihydroxylcoumarin (V) 1, 3,5-trihydroxyhenzene (VI) and xanthotoxol (VII). CONCLUSION: Except compound III, all compounds are isolated from the young fruits of Citrus maxima cv. Shatian for the first time.

4-Amino-3,5-dichloro-benzene-sulfonamide.

In the title compound, C(6)H(6)Cl(2)N(2)O(2)S, the O atoms of the sulfonamide group lie on one side of the benzene ring and the amino group lies on the opposite side. An inter-molecular N-H⋯Cl inter-action occurs. In the crystal, adjacent mol-ecules are linked by N-H⋯O hydrogen bonds, forming a three-dimensional structure with supporting π-π stacking inter-actions [centroid-centroid distance = 3.7903 (12) Å]. A short Cl⋯Cl contact [3.3177 (10) Å] also occurs.