ABSTRACT
OBJECTIVE: To investigate the expression of B cell specific MLV integration site-1 (Bmi-1) in colorectal cancer (CRC) and its correlation to the clinicopathological features and prognosis of CRC. METHODS: Sixty CRC, 30 adenomas and 20 normal colorectal mucosal tissues were collected to detect the expression of Bmi-1 protein using immunohistochemistry, and the results were analyzed in comparison with the clinicopathological features and survival rate of patients. RESULTS: The positivity rate of Bmi-1 expression in CRC tissue was 51.7%. In CRC, the rate of Bmi-1 overexpression was 25.0%, significantly higher than that in the adenomas and normal colorectal mucosal tissues (6.67% and 0%, respectively, P<0.05). The overexpression of Bmi-1 protein in CRC was obviously associated with distant metastasis and the TNM stage (P<0.05), but not with gender, age, tumor size, tumor site, histological type, differentiation degree and lymph node metastasis (P>0.05). But logistic regression analysis showed that Bmi-1 protein overexpression in CRC was associated only with distant metastasis (P<0.01,OR>1); Kaplan-Meier survival analysis showed that the survival rate of the patients with high Bmi-1 expression was significantly lower than that in patients with low expression (P<0.05). CONCLUSION: The overexpression of Bmi-1 protein was significantly correlated to the tumorigenesis, metastasis and prognosis of CRC, and may serve as an indicator for evaluating the prognosis of CRC.
Subject(s)
Colorectal Neoplasms/metabolism , Colorectal Neoplasms/pathology , Nuclear Proteins/metabolism , Proto-Oncogene Proteins/metabolism , Repressor Proteins/metabolism , Adult , Aged , Case-Control Studies , Female , Humans , Male , Middle Aged , Neoplasm Metastasis , Nuclear Proteins/genetics , Polycomb Repressive Complex 1 , Prognosis , Proto-Oncogene Proteins/genetics , Repressor Proteins/genetics , Young AdultABSTRACT
BACKGROUND & OBJECTIVE: Bmi-1, a putative oncogene, is a member of the polycomb group genes, and is expressed in many human tumors. Ki67 is an important nuclear antigen associated with cell proliferation. This study was to investigate the expression, significance and correlation of Bmi-1 and Ki67 in colorectal carcinoma (CRC) tissues. METHODS: Protein expressions of Bmi-1 and Ki67 in sixty CRC, 30 adenomas and 20 normal colorectal mucosal tissues were detected using immunohistochemistry. Correlations of the expression of Bmi-1 and Ki67 to clinicopathological features and survival of patients were analyzed. RESULTS: The expression rates of Bmi-1 and Ki67 were 25.0%, 6.7%, 0% and 18.3%, 3.3% and 0% in CRC, adenomas and normal colorectal mucosal tissues, respectively. The expression of Bim-1 and Ki67 were significantly higher in CRC than in adenomas and normal colorectal mucosal tissues (P<0.05). Chi-square test revealed that the overexpression of Bmi-1 protein in CRC was correlated to distant metastasis (P<0.01) and TNM stage (P<0.05), while the overexpression of Ki67 protein was associated with age, distant metastasis and TNM stage (P<0.05). Further logistic regression analysis showed that only distant metastasis was correlated with Bmi-1 overexpression (P<0.01,OR>1), and only age was correlated with Ki67 overexpression(P<0.05, OR<1). Kaplan-Meier survival analysis showed that the survival was significantly shorter in CRC patients with overexpression than low expression of Bmi-1 and Ki67 (P<0.05). CONCLUSIONS: Overexpression of Bmi-1 and Ki67 protein are significantly correlated with tumorigenesis, metastasis and prognosis of CRC. Bmi-1 and Ki67 might be used to predict the prognosis of CRC. Bmi-1 might regulate the expression of Ki67 indirectly in CRC patients.
Subject(s)
Adenocarcinoma/metabolism , Colorectal Neoplasms/metabolism , Ki-67 Antigen/metabolism , Nuclear Proteins/metabolism , Proto-Oncogene Proteins/metabolism , Repressor Proteins/metabolism , Adenocarcinoma/pathology , Adenocarcinoma, Mucinous/metabolism , Adenocarcinoma, Mucinous/pathology , Adenoma/metabolism , Adenoma/pathology , Adult , Age Factors , Aged , Aged, 80 and over , Colorectal Neoplasms/pathology , Female , Follow-Up Studies , Humans , Intestinal Mucosa/metabolism , Male , Middle Aged , Neoplasm Metastasis , Neoplasm Staging , Polycomb Repressive Complex 1 , Prognosis , Survival Rate , Young AdultABSTRACT
OBJECTIVE: To investigate the change in suppressor of cytokine signaling-1 (SOCS1) gene in the liver and the spleen of septic mouse, and to find out its probable mechanisms of action in sepsis. METHODS: Cecal ligation and puncture (CLP) was adopted to reproduce sepsis model. The liver and the spleen tissues were harvested and RNA and protein were respectively extracted. The contents of the regulatory genes SOCS1 mRNA were determined by reverse transcription-polymerase chain reaction (RT-PCR) and regulatory content of protein was detected by Western blotting. The SPSS statistics software was adopted to calculate the correlation. RESULTS: The expressions of SOCS1 on gene and protein in the liver were markedly upregulated at 6 th hour. The gene expression peaked at the 24 th hour (P<0.05).The expression of protein was persistently high. However, the expression of SOCS1 was only detected in the spleen, and it obviously rose in strength with the passage of time, and it remained in a high level. By statistical analysis, positive correlations were found between the gene and protein expressions of SOCS1 (y=0.110+/-5.765 x 10(-3) x, r=0.837, F=93.309, P<0.01). CONCLUSION: CLP induced sepsis can induce the up-regulation of the expressions of SOCS1, indicating that SOCS1 play important role in the change in immune system in sepsis. They may be used to intervene sepsis so as to improve the outcome of sepsis.
