Ride-On Cars With Different Postures and Motivation in Children With Disabilities: A Randomized Controlled Trial.

IMPORTANCE: A child's independent mobility, environments, and mastery motivation are critical factors during early development. OBJECTIVE: To examine the effectiveness of ride-on car (ROC) training with a standing (ROC-Stand) or a sitting posture (ROC-Sit) in enhancing children's mastery motivation and decreasing parenting stress levels. DESIGN: Randomized controlled trial (RCT) with a multiple pretest-posttest design. SETTING: Hospital-based environment in northern Taiwan. PARTICIPANTS: Thirty-nine children with disabilities ages 1 to 3 yr were randomly assigned to ROC-Stand (n = 16), ROC-Sit (n = 12), or conventional therapy (control; n = 11). All groups received 2-hr training sessions two times a week for 12 wk and then a 12-wk follow-up period that involved only regular therapy. MEASURES: Assessments included the Revised Dimensions of Mastery Questionnaire-Chinese version and the Parenting Stress Index. RESULTS: All groups showed significant changes in social persistence with adults, mastery pleasure, and general competence after the intervention. The two ROC training groups showed a significantly greater decrease in parenting stress than the control group. In addition, increased general competence of the ROC-Stand group also strongly correlated with decreased parent-child dysfunctional interaction. CONCLUSIONS AND RELEVANCE: This RCT verifies the effectiveness of ROC training and offers a novel approach to increase children's mastery motivation and decrease parenting stress. What This Article Adds: Providing a large amount of active, exploratory experiences with goal-directed, moderately challenging tasks and cooperation with caregivers may result in the greatest benefits to young children with motor disabilities.

Joining Illumina paired-end reads for classifying phylogenetic marker sequences.

BACKGROUND: Illumina sequencing of a marker gene is popular in metagenomic studies. However, Illumina paired-end (PE) reads sometimes cannot be merged into single reads for subsequent analysis. When mergeable PE reads are limited, one can simply use only first reads for taxonomy annotation, but that wastes information in the second reads. Presumably, including second reads should improve taxonomy annotation. However, a rigorous investigation of how best to do this and how much can be gained has not been reported. RESULTS: We evaluated two methods of joining as opposed to merging PE reads into single reads for taxonomy annotation using simulated data with sequencing errors. Our rigorous evaluation involved several top classifiers (RDP classifier, SINTAX, and two alignment-based methods) and realistic benchmark datasets. For most classifiers, read joining ameliorated the impact of sequencing errors and improved the accuracy of taxonomy predictions. For alignment-based top-hit classifiers, rearranging the reference sequences is recommended to avoid improper alignments of joined reads. For word-counting classifiers, joined reads could be compared to the original reference for classification. We also applied read joining to our own real MiSeq PE data of nasal microbiota of asthmatic children. Before joining, trimming low quality bases was necessary for optimizing taxonomy annotation and sequence clustering. We then showed that read joining increased the amount of effective data for taxonomy annotation. Using these joined trimmed reads, we were able to identify two promising bacterial genera that might be associated with asthma exacerbation. CONCLUSIONS: When mergeable PE reads are limited, joining them into single reads for taxonomy annotation is always recommended. Reference sequences may need to be rearranged accordingly depending on the classifier. Read joining also relaxes the constraint on primer selection, and thus may unleash the full capacity of Illumina PE data for taxonomy annotation. Our work provides guidance for fully utilizing PE data of a marker gene when mergeable reads are limited.

Efficient speckle reduction for a laser illuminating on a micro-vibrated paper screen.

We have demonstrated an efficient speckle reduction method for laser illumination using a micro-vibrated paper screen along the projection direction. Using this method, a micro-vibrated amplitude of 0.532 µm, or a 2π phase change is sufficient to de-correlate the generated speckle pattern for a static SHG green laser beam image. When the measured speckle contrast is lowered to about 5.0%, and comparable with an LED source, a speckle-free image can be achieved. This technique will be suitable for compact and portable laser illumination equipment, image display, signal sensing devices, and so forth. Wearable and near-to-eye laser display applications are also included.

Visualization of the structures of the hepatitis C virus replication complex.

Hepatitis C viral RNA synthesis has been demonstrated to occur on a lipid raft membrane structure. Lipid raft membrane fraction purified by membrane flotation analysis was observed using transmission electron microscopy and atomic force microscopy. Particles around 0.7 um in size were found in lipid raft membrane fraction purified from hepatitis C virus (HCV) replicon but not their parental HuH7 cells. HCV NS5A protein was associated with these specialized particles. After several cycles of freezing-thawing, these particles would fuse into larger sizes up to 10 um. Knockdown of seven proteins associated with lipid raft (VAPA, COPG, RAB18, COMT, CDC42, DPP4, and KDELR2) of HCV replicon cells reduced the observed number of these particles and suppressed the HCV replication. Results in this study indicated that HCV replication complexes with associated lipid raft membrane form distinct particle structures of around 0.7 um as observed from transmission electron microscopy and atomic force microscopy.

Dry deposition of sulfate-containing particulate at the highway intersection, coastal and suburban areas.

Sulfate-containing aerosol (SCA) dry deposition at the highway intersection, coastal location, and suburban area in Taiwan were analyzed and compared. Sampling was accomplished with a surrogate surface technique. Samples particles were coated with barium chloride (BaCl(2)) in a vacuum evaporator and then exposed to a relative humidity of 85% for 2 h to form distinctive products of SCAs. Treated samples were examined by a scanning electron microscopy. SCA dry deposition fluxes were 10.2, 4.1, 3.4 microgm(-2)s(-1) and nonsulfate-containing aerosol (NSCA) dry deposition fluxes were 23.3, 8.2, 13.5 microgm(-2)s(-1) at the highway intersection, coastal, and suburban areas. At the highway intersection, both SCA and NSCA dry deposition fluxes were much higher than those at the other two sites. The dry deposition of particles was also analyzed with a traditional technique. The number median diameters (NMDs) of SCA were 0.41, 0.82, and 1.2 mum at the highway intersection, coastal, and suburban sites, respectively. The highway intersection site had a small NMD, which showed that most sulfate-containing deposited aerosols existed in fine diameter range. The mass median diameters (MMDs) of SCA were 8.8, 19.5, and 14.9 mum at the highway intersection, coastal, and suburban sites, which were much higher than NMDs. Average numbers of SCAs in total particulate were 33%, 33%, and 22% at the highway intersection, coastal and suburban areas Most deposited particulates were nonsulfate-containing at the three sampling sites. SCAs less than 10 mum contributed 29%, 8%, and 7% to the total dry deposition at the highway intersection, coastal, and suburban areas, respectively. The contribution of fine particulate was significantly higher at the highway intersection site.