Identification of a ceRNA Network Driven by Copy Number Variations in Esophageal Cancer.

BACKGROUND: Copy number variation (CNV) is associated with progression of esophageal cancer (EC), a common gastrointestinal neoplasm. METHODS: Using sequencing data, CNV data, and clinical data of EC transcriptome samples obtained from public databases, we performed differential expression analysis on sequencing data. Differentially expressed CNV-driven lncRNAs were screened using the chi-square test, and CNV-driven lncRNA-associated miRNAs and mRNAs were predicted. Cytoscape software was then used to construct ceRNA networks. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to investigate biological functions of mRNAs in the ceRNA network. Survival curves were plotted to explore correlations between lncRNAs in the ceRNA network and overall survival of CNV patients. Multiple databases were used to predict lncRNAs-related drugs. RESULTS: A dysregulated lncRNA-associated ceRNA network driven by CNV in EC, including 11 lncRNAs, 11 miRNAs and 159 mRNAs, was constructed. Downstream enrichment of mRNAs was related to biological processes such as extracellular matrix organization, indicating that these mRNAs mainly participate in intercellular exchange between tumor cells. Additionally, expression of all lncRNAs in the ceRNA network, except LINC00950, LINC01270 and MIR181A1HG, was correlated with patients' CNV. In addition, none of the 11 lncRNAs was significantly correlated with overall survival of CNV patients. CH5424802 and PD-033299CNV mainly affected the RTK signaling pathway and the cell cycle of tumor cells via RP11-180N14.1 and RP11-273 G15.2 in the ceRNA network. CONCLUSIONS: This study identified 11 CNV-driven lncRNAs that might affect EC development, 2 of which have promising effects if applied to drug treatment. These findings might assist in identifying novel treatments for EC.

Love Forgiveness and Subjective Well-Being in Chinese College Students: The Mediating Role of Interpersonal Relationships.

Love forgiveness is categorized as forgiveness in a specific relationship, which is the tendency of individuals to forgive the objects of their interpersonal relationships. We investigated 831 undergraduate students in China with a love forgiveness questionnaire, a subjective well-being questionnaire and an interpersonal relationship comprehensive diagnostic, including demographic variables. Students of different genders and grades showed significant differences across the three questionnaires. There were significant correlations between love forgiveness, interpersonal relationships, and subjective well-being of Chinese college students. Interpersonal relationships played an intermediary role between love forgiveness and subjective well-being of Chinese college students. Specifically, whereas Chinese college students' love forgiveness could directly promote the improvement of subjective well-being, love forgiveness could also indirectly improve subjective well-being through interpersonal relationships. The mediating effect of interpersonal relationships between love forgiveness and subjective well-being accounted for 40.52% of the total effect. This provides a new way of thinking for psychological counselors to approach the intimate relationship problems of college students.

Relationship between Chinese children's imaginary companions and their understanding of second-order false beliefs and emotions.

We investigated relationship between Chinese children's imaginary companions (ICs) and their understanding of second-order false beliefs and emotions in 180 children, aged 5-6 years old. We examined the potential differences in second-order false belief understanding and emotion understanding between children with and without ICs, children with egalitarian IC relationships and hierarchical IC relationships, as well as children with invisible friends and personified objects. The results revealed that children with ICs had better second-order false belief understanding and emotion understanding than children without ICs. Compared with children with hierarchical relationships, children with egalitarian relationships had better second-order false belief understanding. However, children with invisible friends and personified objects did not differ on their understanding of second-order false beliefs and emotions. The results suggest that compared with IC types, IC status and child-IC relationship qualities may be more relevant to children's theory of mind. It will be interesting for the future researchers to investigate the underlying mechanism of the differences between children with egalitarian IC relationships and hierarchical IC relationships.

Chinese children's imaginary companions: Relations with peer relationships and social competence.

We investigated relationships between Chinese children's imaginary companions (ICs) and peer relationships and social competence in 160 children, aged 5-6 years old. Children and their mothers participated in the interviews regarding the details of the children's ICs, including the type of the companion and the quality of the child-IC relationship. Peer relationships were assessed using sociometric nomination and perceived popularity nomination. Teachers rated children's social competence. Here, 55 children (34.3%) were deemed to have engaged in imaginary companion play. There was no relationship between imaginary companion types and child-IC relationship qualities. Children with invisible friends received more positive nominations than children with personified objects. Children with egalitarian relationships received more positive nominations and popularity nominations, but fewer negative nominations and unpopularity nominations than children with hierarchical relationships. Compared with children with hierarchical relationships, teachers rated the children with egalitarian relationships higher in social competence. The results suggest that imaginary companion types and relationship qualities may represent different dimensions of imaginary companions, calling attentions to the different mechanisms underlying imaginary companion types and relationship qualities with respect to social functioning.

C2H2 adsorption in three isostructural metal-organic frameworks: boosting C2H2 uptake by rational arrangement of nitrogen sites.

Replacing the benzene spacer in the organic linker 5,5'-(benzene-1,4-diyl)diisophthalate with the nitrogen containing heterocyclic rings, namely, pyrazine, pyridazine, and pyrimidine results in three organic linkers, which were reacted with copper ions under solvothermal conditions to form three isostructural metal-organic frameworks (ZJNU-46, ZJNU-47 and ZJNU-48) exhibiting exceptionally high sorption capacities with regard to acetylene due to the simultaneous immobilization of open metal sites and Lewis basic nitrogen sites in the frameworks. At 1 atm and 295 K, the gravimetric C2H2 adsorption uptakes reach 187, 213 and 193 cm(3) (STP) g(-1) for these three compounds. The gravimetric C2H2 adsorption amount of ZJNU-47a is the second highest reported for MOF materials. Notably, despite their same porosities, and densities of open metal sites and uncoordinated nitrogen sites, distinctly different C2H2 adsorption capacities were observed for these three compounds, which we think are mainly associated with the difference in the relative position of nitrogen atoms leading to different binding affinities of the frameworks towards C2H2 guest molecules, and thus different C2H2 adsorptions. This work demonstrates that the rational arrangement of open nitrogen sites will favorably improve the C2H2 uptake and thus provides useful information for future design of porous MOFs with high acetylene storage capacities.