ABSTRACT
Premature ovarian failure (POF) is a clinical term used to describe a condition in which women present with amenorrhoea, hypergonadotropic hypogonadism, and infertility under 40 years old, which are mainly characterized by ovarian granulosa cell inflammation and death. Pyroptosis is a proinflammatory form of programmed cell death. However, the roles of pyroptosis in POF and moxibustion (Mox) on pyroptosis in POF have not been elucidated. The aim of the present study was to investigate the protective effect of moxibustion against cyclophosphamide- (CP-) induced POF and to determine the underlying mechanisms. The results indicated that Mox could decrease the follicle-stimulating hormone (FSH) and luteotropic hormone (LH) and increase estradiol (E2) in serum, which indicated that it could improve ovarian reserve capacity. Mox also ameliorated CP-induced ovarian injury accompanied by decreased levels of interleukin-1ß (IL-1ß), IL-18, and gasdermin D (GSDMD), which are key features of pyroptosis. Further investigation showed that Mox alleviated POF through NLRP3-mediated pyroptosis. On the one hand, Mox directly inhibited TXNIP/NLRP3/caspase-1 signaling-induced pyroptosis, and on the other hand, it indirectly decreased NLRP3, pro-IL-1ß, and pro-IL-18 through inhibiting TLR4/MyD88/NF-κB signaling. Our results show that Mox might be a new therapeutic strategy for the treatment of POF.
ABSTRACT
The increasing concentration of surface ozone (O3) was observed during recent decades in the world, which affects tree roots and forest soils. Meanwhile, the impact of ozone on tree roots is greatly affected by soil condition. However, there is a lack of knowledge about the possible effects of ozone on tree roots and soil processes. In this study, The influences of surface ozone (O3) stress on the root biomass, morphology, nutrients, soil properties, and soil enzyme activity of Elaeocarpus sylvestris and Michelia chapensis seedlings were examined at four O3 concentrations (charcoal-filtered air, 1 × O3 air, 2 × O3 air, and 4 × O3 air). Elevated O3 concentrations were found to significantly increase the root C content, N content, C/P ratio, and N/P ratio, and significantly decrease the root biomass, number of root tips, and root C/N ratio of both species. The soil organic matter content, pH, total N content, and urease and catalase activities of both species tended to increase. The limitation in root growth and responses in the root structure of E. sylvestris induced by elevated O3 concentrations led to increased bulk density and decreased soil porosity and void ratio. These profound effects of O3 concentrations on the roots and soil characteristics of these two species underscore the importance of research in O3 science.
