Olfactory preference of the litchi fruit borer for oviposition on two litchi varieties.

BACKGROUND: The litchi fruit borer Conopomorpha sinensis Bradley is a major destructive pest of litchi and longan plants in China, India and South East Asia. Given its strong olfactory-based oviposition behaviour, interfering with the chemical communication between this insect pest and its host plant may serve as a potential control strategy. However, the chemical compounds associated with its egg-laying behaviour remain poorly understood. RESULTS: In this study, we investigated the olfactory preference of female C. sinensis for oviposition on intact mature fruits of the Feizixiao (FZX) and Guiwei (GW) varieties. Results showed that female C. sinensis preferred to lay eggs on FZX compared with GW fruits, and this preference was olfactory-induced. In addition, we identified differences in the chemical composition of the volatile blend and proportions between FZX and GW fruits, with terpenes being the main volatile components contributing to this divergence. Compounds that induced electrophysiological activity in female borers were subsequently screened from FZX. d-Limonene exhibited the strongest oviposition attraction among four candidates. Furthermore, this compound served as a volatile olfactory cue for recognition and orientation in female C. sinensis. CONCLUSION: The results of this study provide a deeper understanding of the olfactory preferences of female C. sinensis for oviposition on specific litchi varieties. © 2024 Society of Chemical Industry.

Antibacterial Conductive Collagen-Based Hydrogels for Accelerated Full-Thickness Wound Healing.

Antibacterial conductive hydrogels have been extensively utilized in tissue repair and regeneration on account of their unique electrochemical performances and advantages of anti-pathogenic bacterial infection. Here, multi-functional collagen-based hydrogels (CHLY) with adhesivity, conductivity, and antibacterial and antioxidant activities were developed by introducing cysteine-modified ε-poly(l-lysine) (ε-PL-SH) and in situ-polymerized polypyrrole (PPy) nanoparticles to induce full-thickness wound healing. CHLY hydrogels have a low swelling ratio, good compressive strength, and viscoelasticity due to chemical crosslinking, chelation, physical interaction, and nano-reinforcements in the matrix network of hydrogels. CHLY hydrogels possess excellent tissue adhesion ability, low cytotoxicity, enhanced cell migration ability, and good blood coagulation performance without causing hemolysis. Interestingly, the chemical conjugation of ε-PL-SH in the hydrogel matrix gives hydrogels an inherently robust and broad-spectrum antibacterial activity, while the introduction of PPy endows hydrogels with superior free radical scavenging capacity and good electroactivity. Significantly, CHLY hydrogels have advantages in alleviating persistent inflammatory response as well as promoting angiogenesis, epidermis regeneration, and orderly collagen deposition at the wound sites through their multi-functional synergies, thus effectively accelerating full-thickness wound healing and improving wound healing quality. Overall, our developed multi-functional collagen-based hydrogel dressing demonstrates promising application prospects in the field of tissue engineering to induce skin regeneration.

Functionalization of an Electroactive Self-Healing Polypyrrole-Grafted Gelatin-Based Hydrogel by Incorporating a Polydopamine@AgNP Nanocomposite.

Hydrogels are considered a promising wound dressing owing to their ability to absorb wound exudates and their moist network structure for skin regeneration. It is of great significance to give added multiple functions to hydrogels for wound healing. In this paper, we present a gelatin-based hydrogel with self-healing ability, conductivity, and antibacterial and antioxidant activities. Dopamine was added into an alkaline solution to polymerize into polydopamine (PDA), which was used to reduce AgNO3 into Ag nanoparticles (AgNPs) to gain a PDA@AgNP composite. Polypyrrole-grafted gelatin (PPyGel) was dissolved in a PDA@AgNP solution and ferric ions were used as a cross-linking agent to form PDA@AgNPs-PPyGel-Fe hydrogels. The as-prepared hydrogels are soft and ductile and exhibit porous structures with pore sizes from 20 to 50 µm. The hydrogels have high water absorption ability, indicating the potential to absorb wound exudates. PPy and Fe3+ endow the hydrogels with slightly higher conductivity than that of skin tissue, indicating the ability to effectively transmit bioelectric signals for skin regeneration. The ionic interactions and hydrogen bonding in hydrogels make them possess self-healing ability, and the self-healing process can be completed in 30 min. PDA confers hydrogels with effective antioxidant activities, while AgNPs endow hydrogels with good antibacterial activities. Moreover, the hydrogels possess good blood compatibility and cytocompatibility. In sum, the developed hydrogel has potential applications as wound dressings.

Target of rapamycin (TOR) determines appendage size during pupa formation of the red flour beetle Tribolium castaneum.

The adult body size is species-specific and controlled by complex interactions between hormones and the IIS/TOR pathway. To analyze the role of target of rapamycin (TOR) in the growth and development of the insect, expression levels of TOR were silenced in the model and pest insect red flour beetle, Tribolium castaneum. Injection of dsRNA into the last larval instar decreased pupal mass and size, while the amount of food intake by the larvae was not affected. These results place TcTOR downstream of nutrition as a transducer for nutritional signals to increase larval growth. In addition, TcTOR-silencing notably decreased the size of the adult appendages. Analysis of the wings and elytra revealed a decrease in cell size and number of these appendages in the TcTOR-silenced insects. This reduction in size was correlated with a decrease of transcriptional levels of marker genes controlling the cell cycle. Altogether, these results suggest a pivotal role for TcTOR in integrating nutritional signals and regulation of body and appendages growth.

Flexible thermal responsive infrared reflector based on cholesteric liquid crystals and polymer stabilized cholesteric liquid crystals.

In this study, we fabricated a temperature-responsive infrared reflector that adjusts to temperature changes by changing its transmittance of incident IR light. The device utilized a thermally induced change in the pitch of a cholesteric liquid crystal (CLC) to achieve near-infrared light reflection in a particular wavelength range. In addition, a polymer-stabilized cholesteric liquid crystal (PSCLC) was used as an alternative to further optimize the device performance. Polyethylene terephthalate (PET) was used as the substrate material to allow the reflector to be flexible. The light transmission performance of the reflector at different bending angles was explored, and no significant effect was found. A simulated solar device was established to study the temperature regulation effects of both CLC and PSCLC devices.

Roles of the insulin signaling pathway in insect development and organ growth.

Organismal development is a complex process as it requires coordination of many aspects to grow into fit individuals, such as the control of body size and organ growth. Therefore, the mechanisms of precise control of growth are essential for ensuring the growth of organisms at a correct body size and proper organ proportions during development. The control of the growth rate and the duration of growth (or the cessation of growth) are required in size control. The insulin signaling pathway and the elements involved are essential in the control of growth. On the other hand, the ecdysteroid molting hormone determines the duration of growth. The secretion of these hormones is controlled by environmental factors such as nutrition. Moreover, the target of rapamycin (TOR) pathway is considered as a nutrient sensing pathway. Important cross-talks have been shown to exist among these pathways. In this review, we outline the control of body and organ growth by the insulin/TOR signaling pathway, and also the interaction between nutrition via insulin/TOR signaling and ecdysteroids at the coordination of organismal development and organ growth in insects, mainly focusing on the well-studied fruit fly Drosophila melanogaster.

Importation of Mumps Virus Genotype K to China from Vietnam.

During May-August 2016, mumps virus genotype K was detected in 12 Vietnam citizens who entered China at the Shuikou border crossing and 1 girl from China. We provide evidence that mumps genotype K is circulating in Vietnam and was imported to China from Vietnam.

FoxO mediates the timing of pupation through regulating ecdysteroid biosynthesis in the red flour beetle, Tribolium castaneum.

The steroid hormone 20-hydroxyecdysone (20E), the major developmental hormone in insects, controls all the developmental transitions including ecdysis and metamorphosis. In our study with last larval stages of the red flour beetle, Tribolium castaneum, dsRNA-mediated gene silencing of Forkhead box protein O (FoxO) resulted in reduced food intake and larval mass and this agreed with a reduction in the expression of insulin signaling-related genes (insulin-like peptides 2, 3, 4, and chico). Interestingly, we also observed a significant delay in the moment of the pupation and these FoxO-silenced larvae then turned brown at the middle pupal stage followed by death. The observed delay of pupation concurred with a significant delay in 20E titer in dsFoxO-injected larvae and this in turn agreed with a significant delay in expression of prothoracicotropic hormone (ptth) that is a gene stimulating ecdysteroid biosynthesis, and of spook (spo) that is one of the early Halloween genes involved in ecdysteroid biosynthesis. In addition, there was also a delayed expression of the ecdysteroid response gene hormone receptor 3 (HR3). In an attempt to rescue the effects by dsFoxO, injection of 20E into T. castaneum larvae stimulated the expression of HR3 and induced one extra larval-larval molt, confirming the responsiveness for ecdysteroid signaling in dsFoxO-injected larvae. The observations of this project suggest that FoxO is a player in the timing of pupation via the regulating of ecdysteroid biosynthesis, together with the regulation of both insulin signaling and nutrition.

20-hydroxyecdysone positively regulates the transcription of the antimicrobial peptide, lebocin, via BmEts and BmBR-C Z4 in the midgut of Bombyx mori during metamorphosis.

Metamorphosis is an essential physiological process in insects. This process is triggered by 20-hydroxyecydsone (20E). Lebocin, an antimicrobial peptide of Lepidoptera insects, was significantly up-regulated in the midgut, but not in the fat body of Bombyx mori during metamorphosis. In this study, the expression regulation of lebocin in B. mori midgut was studied. The results showed that B. mori lebocin and its activator BmEts were not responsive to bacterial infection in the midgut, instead, the expression of both genes was up-regulated by 20E treatment. The transcription factor BR-C Z4 in the 20E signal pathway enhanced lebocin promoter activity by directly binding to an upstream cis-response element of the promoter. In the fat body, the mRNA level of B. mori lebocin was decreased when the insect transformed from larval to pupal stage and was increased by immune challenge. The expression profiles of lebocin in Lepidopteran Spodoptera litura was also analyzed and the similar results were observed, S. litura lebocin was significantly up-regulated during midgut regeneration and mainly present in the new-formed intestinal cells of the midgut. All results together suggest that during metamorphosis 20E may activate lebocin expression via BmBR-C Z4 and BmEts in the midgut, where the antimicrobial peptide was produced to protect the midgut from infection.

Insulin receptor regulates food intake through sulfakinin signaling in the red flour beetle, Tribolium castaneum.

Insects obtain energy and nutrients via feeding to support growth and development. The insulin signaling pathway is involved in the regulation of feeding; however, the underlying mechanisms are not fully understood. Here, we show that insulin signaling regulates food intake via crosstalk with neuropeptide sulfakinin in the red flour beetle, Tribolium castaneum. Silencing of the insulin receptor (InR) decreased the food intake in the penultimate and final instar stages, leading to a decrease of weight gain and mortality during larval-pupal metamorphosis. Interestingly, the knockdown of InR co-occurred with an increased expression of sulfakinin (sk), a gene encoding neuropeptide SK functioning as a satiety signal. In parallel, double silencing of sk and InR eliminated the inhibitory effect on food intake as induced by silencing of InR and the larvae died as prepupae. In conclusion, this study shows, for the first time, that the insulin/InR signaling regulates food intake through the sulfakinin signaling pathway in the larval stages of this important model and pest insect, indicating a novel target for pest control.

Structure and expression of glutathione S-transferase genes from the midgut of the Common cutworm, Spodoptera litura (Noctuidae) and their response to xenobiotic compounds and bacteria.

Glutathione S-transferases (GSTs) play a pivotal role in detoxifying endogenous and xenobiotic compounds and oxidative stress resistance in cells. In this study, five GST genes, including three Sigma GSTs (SlGSTs1, SlGSTs2, and SlGSTs3), one Omega GST (SlGSTo1) and one un-classified GST (SlGSTu1) were identified from the midgut of the Common cutworm, Spodoptera litura. Structure analyses of the eight (including the previously identified Epsilon GST genes, SlGSTe1, SlGSTe2 and SlGSTe3 from the same insect) SlGSTs genes showed that the Epsilon SlGSTe genes do not contain any intron, while the Sigma SlGSTs contain three introns and the Omega SlGSTo1 and the un-classified SlGSTu1 contain five introns. Analysis of the spatial and temporal expression of these eight SlGSTs indicated that SlGSTe1, SlGSTs2 and SlGSTo1 expressed in all stages of development from the egg to the adult stages. SlGSTe2, SlGSTe3, SlGSTs1, SlGSTs3 and SlGSTu1 had higher expression levels in the larval stages than in other stages and their expression levels in the midgut were higher than in other tissues. SlGSTs1 was expressed in the larval midgut but not in the fat body and could be induced by bacterial infections. The expression of SlGSTe1, SlGSTe3, SlGSTs1 and SlGSTs3 was increased by chlorpyrifos to various degrees, while the expression of SlGSTe1, SlGSTe3, SlGSTs1, SlGSTs3 and SlGSTo1 was increased by xanthotoxin. Levels of malonaldehyde, an indicator of oxidative stress, were higher in the larval midgut than in the pupal midgut. Chlorpyrifos induced the malonaldehyde content in the larvae, whereas xanthotoxin did not. It is hypothesized that high expression levels of the midgut SlGSTs might be due to the increased levels of oxidative stress caused by feeding, bacterial infection and xenobiotic compounds.