Prediction of Receptor Status in Radiomics: Recent Advances in Breast Cancer Research.

Breast cancer is a multifactorial heterogeneous disease and the leading cause of cancer-related deaths in women; its diagnosis and treatment require clinical sensitivity and a comprehensive disciplinary research approach. The expression of different receptors on tumor cells not only provides the basis for molecular typing of breast cancer but also has a decisive role in the diagnosis, treatment, and prognosis of breast cancer. To date, immunohistochemistry (IHC), which uses invasive histological sampling, has been extensively used in clinical practice to analyze the status of receptors and to make an accurate diagnosis of breast cancer. As an invasive assay, IHC can provide important biological information on tumors at a single point in time, but cannot predict future changes (due to treatment or tumor mutations) without additional invasive procedures. These issues highlight the need to develop a non-invasive method for predicting receptor status. The emerging field of radiomics may offer a non-invasive approach to identification of receptor status without requiring biopsy. In this paper, we present a review of the latest research results in radiomics for predicting the status of breast cancer receptors, with potential important clinical applications.

Synthesis of Thifluzamide Artificial Antigens and Establishment of Enzyme Linked Immunosorbent Assay Detection Method / 分析化学

In the present study, 2-methyl-4-( trifluoromethyl) thiaZole-5-carboxylic acid ( MTCA) was acted as hapten of thifluZamide to synthesiZe artificial antigens.Immunogen MTCA-bovine serum albumin ( BSA ) was synthesiZed by active ester method.Meanwhile, three coating antigens, MTCA-OVA-1, MTCA-OVA-2 and MTCA-OVA-3 were synthesiZed by active ester method, mixed anhydride method and N, N-carbonyldiimidaZole/4-dimethylaminopyridine ( CDI/DMAP ) method, respectively.Anti-thifluZamide polyclonal antibody with high specificity was obtained from the immuniZed mice, then indirect competitive enZyme linked immunosorbent assay ( ic-ELISA ) method for thifluZamide detection was developed by using MTCA-OVA-3 and polyclonal antibody.The linear detection range in ic-ELISA was 0.08-10 mg/L with an IC50 of 1.39 mg/L and a LOD (IC10) of 0.08 mg/L.The recoveries for spiked samples including tap water, lake water and wheat ranged from 72.0%-128.3% in ic-ELISA method.Good correlation (R2=0.9994) was obtained between the results of ic-ELISA and those of HPLC analysis.The proposed ic-ELSA was promising for rapid detection of thifluZamide in water and agricultural products.