Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Sci Rep ; 11(1): 2645, 2021 01 29.
Article in English | MEDLINE | ID: mdl-33514791

ABSTRACT

The initiator caspase Dronc is the only CARD-domain containing caspase in Drosophila and is essential for apoptosis. Here, we report that homozygous dronc mutant adult animals are short-lived due to the presence of a poorly developed, defective and leaky intestine. Interestingly, this mutant phenotype can be significantly rescued by enteroblast-specific expression of dronc+ in dronc mutant animals, suggesting that proper Dronc function specifically in enteroblasts, one of four cell types in the intestine, is critical for normal development of the intestine. Furthermore, enteroblast-specific knockdown of dronc in adult intestines triggers hyperplasia and differentiation defects. These enteroblast-specific functions of Dronc do not require the apoptotic pathway and thus occur in a non-apoptotic manner. In summary, we demonstrate that an apoptotic initiator caspase has a very critical non-apoptotic function for normal development and for the control of the cell lineage in the adult midgut and therefore for proper physiology and homeostasis.


Subject(s)
Caspases/biosynthesis , Drosophila Proteins/biosynthesis , Gene Expression Regulation , Intestinal Mucosa/metabolism , Mutation , Animals , Apoptosis , Caspases/genetics , Drosophila Proteins/genetics , Drosophila melanogaster
2.
Cell Rep ; 33(8): 108408, 2020 11 24.
Article in English | MEDLINE | ID: mdl-33238125

ABSTRACT

We reveal surprising similarities between homeostatic cell turnover in adult Drosophila midguts and "undead" apoptosis-induced compensatory proliferation (AiP) in imaginal discs. During undead AiP, immortalized cells signal for AiP, allowing its analysis. Critical for undead AiP is the Myo1D-dependent localization of the initiator caspase Dronc to the plasma membrane. Here, we show that Myo1D functions in mature enterocytes (ECs) to control mitotic activity of intestinal stem cells (ISCs). In Myo1D mutant midguts, many signaling events involved in AiP (ROS generation, hemocyte recruitment, and JNK signaling) are affected. Importantly, similar to AiP, Myo1D is required for membrane localization of Dronc in ECs. We propose that ECs destined to die transiently enter an undead-like state through Myo1D-dependent membrane localization of Dronc, which enables them to generate signals for ISC activity and their replacement. The concept of transiently "undead" cells may be relevant for other stem cell models in flies and mammals.


Subject(s)
Enterocytes/metabolism , Animals , Drosophila , Homeostasis
3.
Dev Cell ; 45(4): 450-464.e3, 2018 05 21.
Article in English | MEDLINE | ID: mdl-29787709

ABSTRACT

Caspases are best characterized for their function in apoptosis. However, they also have non-apoptotic functions such as apoptosis-induced proliferation (AiP), where caspases release mitogens for compensatory proliferation independently of their apoptotic role. Here, we report that the unconventional myosin, Myo1D, which is known for its involvement in left/right development, is an important mediator of AiP in Drosophila. Mechanistically, Myo1D translocates the initiator caspase Dronc to the basal side of the plasma membrane of epithelial cells where Dronc promotes the activation of the NADPH-oxidase Duox for reactive oxygen species generation and AiP in a non-apoptotic manner. We propose that the basal side of the plasma membrane constitutes a non-apoptotic compartment for caspases. Finally, Myo1D promotes tumor growth and invasiveness of the neoplastic scrib RasV12 model. Together, we identified a new function of Myo1D for AiP and tumorigenesis, and reveal a mechanism by which cells sequester apoptotic caspases in a non-apoptotic compartment at the plasma membrane.


Subject(s)
Apoptosis , Caspases/metabolism , Cell Membrane/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/physiology , Myosins/metabolism , Neoplasms, Experimental/pathology , Animals , Cell Proliferation , Drosophila Proteins/genetics , Drosophila melanogaster/enzymology , Male , Membrane Proteins , Myosins/genetics , Neoplasms, Experimental/genetics , Neoplasms, Experimental/metabolism , Signal Transduction , Tumor Suppressor Proteins/genetics , Tumor Suppressor Proteins/metabolism , ras Proteins/genetics , ras Proteins/metabolism
4.
Elife ; 62017 08 30.
Article in English | MEDLINE | ID: mdl-28853394

ABSTRACT

Apoptosis and its molecular mediators, the caspases, have long been regarded as tumor suppressors and one hallmark of cancer is 'Evading Apoptosis'. However, recent work has suggested that apoptotic caspases can also promote proliferation and tumor growth under certain conditions. How caspases promote proliferation and how cells are protected from the potentially harmful action of apoptotic caspases is largely unknown. Here, we show that although caspases are activated in a well-studied neoplastic tumor model in Drosophila, oncogenic mutations of the proto-oncogene Ras (RasV12) maintain tumorous cells in an 'undead'-like condition and transform caspases from tumor suppressors into tumor promotors. Instead of killing cells, caspases now promote the generation of intra- and extracellular reactive oxygen species (ROS). One function of the ROS is the recruitment and activation of macrophage-like immune cells which in turn signal back to tumorous epithelial cells to activate oncogenic JNK signaling. JNK further promotes and amplifies caspase activity, thereby constituting a feedback amplification loop. Interfering with the amplification loop strongly reduces the neoplastic behavior of these cells and significantly improves organismal survival. In conclusion, RasV12-modified caspases initiate a feedback amplification loop involving tumorous epithelial cells and macrophage-like immune cells that is necessary for uncontrolled tumor growth and invasive behavior.


Subject(s)
Caspases/metabolism , MAP Kinase Kinase 4/metabolism , Macrophages/immunology , Neoplasms/physiopathology , Proto-Oncogene Proteins p21(ras)/metabolism , Reactive Oxygen Species/metabolism , Animals , Cell Proliferation , Disease Models, Animal , Drosophila , Epithelial Cells/physiology , Mutant Proteins/genetics , Mutant Proteins/metabolism , Proto-Oncogene Proteins p21(ras)/genetics , Signal Transduction
5.
BMC Biol ; 14: 70, 2016 08 19.
Article in English | MEDLINE | ID: mdl-27542914

ABSTRACT

BACKGROUND: ATG1 belongs to the Uncoordinated-51-like kinase protein family. Members of this family are best characterized for roles in macroautophagy and neuronal development. Apoptosis-induced proliferation (AiP) is a caspase-directed and JNK-dependent process which is involved in tissue repair and regeneration after massive stress-induced apoptotic cell loss. Under certain conditions, AiP can cause tissue overgrowth with implications for cancer. RESULTS: Here, we show that Atg1 in Drosophila (dAtg1) has a previously unrecognized function for both regenerative and overgrowth-promoting AiP in eye and wing imaginal discs. dAtg1 acts genetically downstream of and is transcriptionally induced by JNK activity, and it is required for JNK-dependent production of mitogens such as Wingless for AiP. Interestingly, this function of dAtg1 in AiP is independent of its roles in autophagy and in neuronal development. CONCLUSION: In addition to a role of dAtg1 in autophagy and neuronal development, we report a third function of dAtg1 for AiP.


Subject(s)
Apoptosis , Autophagy-Related Protein-1 Homolog/metabolism , Autophagy , Drosophila Proteins/metabolism , Drosophila/genetics , Animals , Autophagy-Related Protein-1 Homolog/genetics , Cell Proliferation , Drosophila/metabolism , Drosophila Proteins/genetics , Eye/growth & development , Imaginal Discs/growth & development , MAP Kinase Signaling System , Transcriptional Activation , Wings, Animal/growth & development
6.
Curr Biol ; 26(5): 575-84, 2016 Mar 07.
Article in English | MEDLINE | ID: mdl-26898463

ABSTRACT

Apoptosis-induced proliferation (AiP) is a compensatory mechanism to maintain tissue size and morphology following unexpected cell loss during normal development, and may also be a contributing factor to cancer and drug resistance. In apoptotic cells, caspase-initiated signaling cascades lead to the downstream production of mitogenic factors and the proliferation of neighboring surviving cells. In epithelial cells of Drosophila imaginal discs, the Caspase-9 ortholog Dronc drives AiP via activation of Jun N-terminal kinase (JNK); however, the specific mechanisms of JNK activation remain unknown. Here we show that caspase-induced activation of JNK during AiP depends on an inflammatory response. This is mediated by extracellular reactive oxygen species (ROSs) generated by the NADPH oxidase Duox in epithelial disc cells. Extracellular ROSs activate Drosophila macrophages (hemocytes), which in turn trigger JNK activity in epithelial cells by signaling through the tumor necrosis factor (TNF) ortholog Eiger. We propose that in an immortalized ("undead") model of AiP, signaling back and forth between epithelial disc cells and hemocytes by extracellular ROSs and TNF/Eiger drives overgrowth of the disc epithelium. These data illustrate a bidirectional cell-cell communication pathway with implication for tissue repair, regeneration, and cancer.


Subject(s)
Apoptosis , Cell Proliferation , Drosophila melanogaster/metabolism , Macrophages/metabolism , Reactive Oxygen Species/metabolism , Animals , Caspases/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/enzymology , Drosophila melanogaster/growth & development , JNK Mitogen-Activated Protein Kinases/metabolism , Larva
7.
PLoS Genet ; 10(1): e1004131, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24497843

ABSTRACT

Recent work in several model organisms has revealed that apoptotic cells are able to stimulate neighboring surviving cells to undergo additional proliferation, a phenomenon termed apoptosis-induced proliferation. This process depends critically on apoptotic caspases such as Dronc, the Caspase-9 ortholog in Drosophila, and may have important implications for tumorigenesis. While it is known that Dronc can induce the activity of Jun N-terminal kinase (JNK) for apoptosis-induced proliferation, the mechanistic details of this activation are largely unknown. It is also controversial if JNK activity occurs in dying or in surviving cells. Signaling molecules of the Wnt and BMP families have been implicated in apoptosis-induced proliferation, but it is unclear if they are the only ones. To address these questions, we have developed an efficient assay for screening and identification of genes that regulate or mediate apoptosis-induced proliferation. We have identified a subset of genes acting upstream of JNK activity including Rho1. We also demonstrate that JNK activation occurs both in apoptotic cells as well as in neighboring surviving cells. In a genetic screen, we identified signaling by the EGFR pathway as important for apoptosis-induced proliferation acting downstream of JNK signaling. These data underscore the importance of genetic screening and promise an improved understanding of the mechanisms of apoptosis-induced proliferation.


Subject(s)
Apoptosis/genetics , Carcinogenesis/genetics , Drosophila Proteins/genetics , ErbB Receptors/genetics , MAP Kinase Kinase 4/genetics , Receptors, Invertebrate Peptide/genetics , rho GTP-Binding Proteins/genetics , Animals , Caspases , Cell Proliferation , Drosophila Proteins/isolation & purification , Drosophila Proteins/metabolism , Drosophila melanogaster , ErbB Receptors/metabolism , Humans , Models, Genetic , Neoplasms/genetics , Neoplasms/pathology , Receptors, Invertebrate Peptide/metabolism , Regeneration/genetics , Wnt Signaling Pathway , rho GTP-Binding Proteins/isolation & purification
SELECTION OF CITATIONS
SEARCH DETAIL
...